Abstract The phloem unloading pathway remains unclear in fleshy fruits accumulating a high level of soluble sugars. A structural investigation apple fruit (Malus domestica Borkh. cv Golden Delicious) showed that the sieve element-companion cell complex sepal bundles feeding flesh is symplasmically isolated over development. 14C-autoradiography indicated was functional for unloading. Confocal laser scanning microscopy imaging carboxyfluorescein dye remained confined to strands from basal...

Lilium lancifolium, a very important cold-resistant wild flower for lily cold resistance breeding, is widely distributed in southwestern and northeastern China. To gain better understanding of the signaling pathway molecular metabolic reactions involved response, we performed genome-wide transcriptional analysis using RNA-Seq.Approximately 104,703 million clean 90- bp paired-end reads were obtained from three libraries (CK 0 h, Cold-treated 2 h 16 at 4 °C); 18,736 unigenes showed similarity...

Genetic diversity in modern upland cotton cultivars ( Gossypium hirsutum L.) is thought to be narrow, thus limiting genetic advance. Robust information on the relatedness among currently grown lacking. The objectives of present study were field test a sample elite commercial cultivars, including many transgenic representing major cottonseed companies, and evaluate their divergence using simple sequence repeat (SSR) markers. Eighty‐eight SSR primer pairs chosen for genotyping that provided...

The New Mexico cotton breeding program was established in 1926 and has been led by five generations of breeders geneticists. released more than 30 Acala 1517 ( Gossypium hirsutum L.) cultivars numerous germplasm lines known for high fiber quality Verticillium wilt (caused dahliae Kleb.) tolerance that have made substantial contributions to the USA. present project initiated 2003 evaluate genetic improvement over past 75 yr yield, boll size, seed index, lint percentage, length, strength,...

Abstract The acid invertase ( β ‐fructosidase, EC 3·2·1·26) was localized at subcellular level via immunogold electron microscopy in the phloem‐unloading zone of developing apple fruit. enzyme (immunogold particles) found to reside predominantly cell walls sieve element/companion (SE/CC) complex, phloem parenchyma cells and other cells. There almost no gold particle cytoplasm vacuole. This distribution pattern remained unchanged throughout growing season, but numbers varied. density...

21 crossing were conducted between Asiatic Lily with different ploidy levels, the results showed that interploidy hybridization diploid and tetraploid lilies was not as successful intraploidy hybridization. Regardless of male sterility, triploid could be used female parents in which progenies aneuploidy. 3x×4x crosses cultured more successfully than 3x×2x crosses. 45S rDNA mapped on chromosomes seven Lilium species their using fluorescence situ (FISH). FISH revealed six to sixteen gene loci,...

Oriental hybrid lily 'Sorbonne', a very important cut flower for lily, is enjoyed great popularity in the world, but it must experience period of low winter temperature to initiate or accelerate flowering process. To gain better understanding signaling pathway and molecular metabolic reactions involved vernalization response, genome-wide transcriptional analysis using RNA-Seq was performed. 188,447,956 sequencing reads assembled into 66,327 unigenes showed similarity known proteins...

Low temperature can affect the growth and development of lily, limiting application commercial cultivars in outdoor. Lilium lancifolium is an important cold-resistant wild but little known about how L. tolerates cold stress at molecular level. In this study, we identified characterized genes transcription factors associated with control plants treated by 4° C for 1 - 24 h. The construction a highest reciprocal rank-based gene co-expression network along its partition into defined functional...

Lily (Lilium spp), which belongs to Lilium, is one kind of monocotyledon. As a perennial ornamental plant with extremely high esthetic, edible, and medicinal value, lily has gained much favor due its mostly showy flowers various colors elegant shape. In this research, we studied experimental materials in sample 49 individuals including 40 cultivars, nine species wild lily, their variants. The collection cultivars covered all six hybrids the genus, i.e., Asiatic hybrids, Oriental Longiflorum...

The Chinese hawthorn (Crataegus pinnatifida Bge.var.major N.E.Br.) is uniquely originated in northern China.The ecological and horticultural importance of considerable some varieties are valued for their fruit or medicine extracts.Its taxonomy phylogeny remain poorly understood.Apart from general plant morphological traits, pollen an important trait the classification plants evolutionary origin.However, few studies have investigated hawthorn.Here, analysis characteristics was conducted 57...

To explore candidate genes responsible for the blue color of Selaginella uncinata (Desv. ex Poir.) Spring leaves, we used transcriptome sequencing to compare differential gene expressions in and red S. leaves selected based on morphological observations pigment content. In total, obtained 30 119 unigenes with an average length 1133 bp, identified 1442 differentially expressed (DEGs). Among DEGs, 886 were upregulated 556 downregulated leaf samples. After verification expression by...

Abstract Background : Rose is one of the most important ornamental crops, but drought limits its yield and quality seriously. Although serval abiotic stress related genes have been identified, molecular mechanism during rose’s responding to still limited. In this study, transcriptomes leaves two-year-old cutting seedlings Rosa chinensis ’Old Blush’ from three continuous droughted stages (30, 60, 90 days after full watering) rewatering were analyzed using RNA sequencing. Meanwhile, weighted...

Ecological balance and metabolism activity are caused by changes in the expression of differential genes proteins when plants subjected to various stresses. Lilium lancifolium is a very important cold-resistant wild flower for lily cold resistance breeding. In order study response mechanism stresses (cold ABA) in-depth, proteome was conducted on basis 2-D gel electrophoresis. One hundred forty-nine significantly protein spots were found DIGE technology, 21 with at least 2-fold altered...