A5089975001- Metabolism, Diabetes, and Cancer
- Cancer Cells and Metastasis
- HER2/EGFR in Cancer Research
- Pancreatic function and diabetes
- Cancer Genomics and Diagnostics
- Breast Cancer Treatment Studies
- Pluripotent Stem Cells Research
- Advanced MRI Techniques and Applications
- PI3K/AKT/mTOR signaling in cancer
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Cell Adhesion Molecules Research
- Epigenetics and DNA Methylation
- Spectroscopy Techniques in Biomedical and Chemical Research
- Radiomics and Machine Learning in Medical Imaging
- Breast Lesions and Carcinomas
- Autophagy in Disease and Therapy
- Ferroptosis and cancer prognosis
- Growth Hormone and Insulin-like Growth Factors
- Monoclonal and Polyclonal Antibodies Research
- Ovarian cancer diagnosis and treatment
- Microtubule and mitosis dynamics
- Silymarin and Mushroom Poisoning
- Sarcoma Diagnosis and Treatment
- Medical Imaging and Pathology Studies
Hospital Universitari de Girona Doctor Josep Trueta
2014-2025
Institut d'Investigació Biomèdica de Girona
2009-2025
Institut d'Investigació Sanitària Pere Virgili
2018
Hospital Universitario de Canarias
2018
Molecular Oncology (United States)
2009-2013
Catalan Institute for Water Research
2011
Institut Català d'Oncologia
2011
When fighting cancer, knowledge on metabolism has always been important. Today, it matters more than ever. The restricted cataloging of cancer genomes is quite unlikely to achieve the task curing unless integrated into metabolic networks that respond and influence constantly evolving stem cell (CSC) cellular states. Once genomic era carcinogenesis had pushed 1920s Otto Warburg's hypothesis obscurity for decades, most recent studies begin support a new developing paradigm, in which molecular...
Aging can be viewed as a quasi-programmed phenomenon driven by the overactivation of nutrient-sensing mTOR gerogene. mTOR-driven aging triggered or accelerated decline loss responsiveness to activation energy-sensing protein AMPK, critical gerosuppressor mTOR. The occurrence age-related diseases, therefore, reflects synergistic interaction between our evolutionary path sedentarism, which chronically increases number activating gero-promoters (e.g., food, growth factors, cytokines and...
The rate of inherent resistance to single-agent trastuzumab in HER2-overexpressing metastatic breast carcinomas is impressive at above 70%. Unfortunately, little known regarding the distinctive genetic signatures that could predict refractoriness ab initio. epithelial-to-mesenchymal transition (EMT) molecular features, HER2 expression status and primary responses were explored public Lawrence Berkeley Laboratory (LBL) Breast Cancer Collection. Lentivirus-delivered small hairpin RNAs employed...
New strategies to block the immune evasion activity of programmed death ligand-1 (PD-L1) are urgently needed.When exploring PD-L1-targeted effects mechanistically diverse metabolism-targeting drugs, exposure dietary polyphenol resveratrol (RSV) revealed its differential capacity generate a distinct PD-L1 electrophoretic migration pattern.Using biochemical assays, computer-aided docking/molecular dynamics simulations, and fluorescence microscopy, we found that RSV can operate as direct...
Autophagy may control the de novo refractoriness of HER2 gene-amplified breast carcinomas to monoclonal antibody trastuzumab (Herceptin). Tumor cells originally obtained from a patient who rapidly progressed on ab initio display increased cellular levels LC3-II protein—a finding that correlates with numbers autophagosomes—and decreased autophagy receptor p62/SQSTM1, protein selectively degraded by autophagy. Trastuzumab-refractory are in state “autophagy addiction” because genetic ablation...
Energy metabolism plasticity enables stemness programs during the reprogramming of somatic cells to an induced pluripotent stem cell (iPSC) state. This relationship may introduce a new era in understanding Warburg's theory on metabolic origin cancer at level (CSCs). Here, we used Yamanaka's technology attempt create stable CSC research lines which dissect transcriptional control mTOR—the master switch cellular catabolism and anabolism—in CSC-like states. The rare colonies with iPSC-like...
Our understanding on how selective mitochondrial autophagy, or mitophagy, can sustain the archetypal properties of stem cells is incomplete. PTEN-induced putative kinase 1 (PINK1) plays a key role in maintenance morphology and function degradation damaged mitochondria by mitophagy. Here, using embryonic fibroblasts fromPINK1 gene-knockout (KO) mice, we evaluated whether mitophagy causal mechanism for control cell-fate plasticity pluripotency. Loss PINK1-dependent was sufficient to...
// Begoña Martin-Castillo 1, * , Sonia Pernas 2, Joan Dorca 3 Isabel Álvarez 4, 5 Susana Martínez 6 Jose Manuel Pérez-Garcia 7 Norberto Batista-López 8 César A. Rodríguez-Sánchez 9, 10 Kepa Amillano 11 Severina Domínguez 12 Maria Luque 13 Agostina Stradella 2 Idoia Morilla Gemma Viñas Javier Cortés 14 Elisabet Cuyàs 15 Sara Verdura Álvaro Fernández-Ochoa 16, 17 Salvador Fernández-Arroyo 18 Antonio Segura-Carretero Jorge Joven Elsa Pérez 19 Neus Bosch 20 Margarita Garcia 21 Eugeni López-Bonet...
Complete digital pathology transformation for primary histopathological diagnosis is a challenging yet rewarding endeavor. Its advantages are clear with more efficient workflows, but there many technical and functional difficulties to be faced. The Catalan Health Institute (ICS) has started its DigiPatICS project, aiming deploy in an integrative, holistic, comprehensive way within network of 8 hospitals, over 168 pathologists, 1 million slides each year. We describe the bidding process...
The antidiabetic drug metformin efficiently circumvents the dilemma that in reducing tumourigenicity of stem cells, their essence, specifically pluripotency, must also be sacrificed. Metformin prevents occurrence or drastically reduces size and weight teratoma-like masses after transplantation induced pluripotent (iPS) cells into immunodeficient mice. Yet, iPS implanted metformin-treated mice retain full as they produce same number distinct tissue types derived from three embryonic germ...
AbstractThe AMP-activated protein kinase (AMPK) system, an evolutionary conserved low-energy checkpoint, functions as a canonical suppressor of cell proliferation. Proliferating cells, however, should also ensure proper spatio-temporal bond between AMPK-sensed cell's metabolic status and division. A crucial linkage proliferation AMPK-interpreted bioenergetics appears to take place during the M-phase cycle. recent description physical interplay active form α-catalytic AMPK subunit with...
High rates of inherent primary resistance to the humanized monoclonal antibody trastuzumab (Herceptin) are frequent among HER2 gene-amplified breast carcinomas in both metastatic and adjuvant settings. The clinical efficacy is highly correlated with its ability specifically efficiently target HER2-driven populations cancer stem cells (CSCs). Intriguingly, many possible mechanisms by which escape involve same biomarkers that have been implicated biology CS-like tumor-initiating cells. In...
Abstract A one-two punch sequential regimen of senescence-inducing agents followed by senolytic drugs has emerged as a novel therapeutic strategy in cancer. Unfortunately, cancer cells undergoing therapy-induced senescence (TIS) vary widely their sensitivity to senotherapeutics, and companion diagnostics predict the response TIS specific drug are lacking. Here, we hypothesized that ability BH3 profiling assay functionally measure mitochondrial priming state—the proximity apoptotic...
The restoration of pluripotency circuits by the reactivation endogenous stemness factors, such as SOX2, may provide a new paradigm in cancer development. tumoral stem cell reprogramming hypothesis, i.e., ability factors to redirect normal and differentiated tumor cells toward less-differentiated stem-like state, adds layers complexity biology, because effects remain dormant until engaged later response (epi)genetic and/or (micro)environmental events. To test this we utilized an vitro model...
Clinically HER2+ (cHER2+) breast cancer (BC) can no longer be considered a single BC disease entity in terms of trastuzumab responsiveness. Here we propose framework for predicting the response cHER2+ to that integrates molecular distinctions intrinsic subtypes with recent knowledge on stem cell (CSC) biology. First, consider two interchangeable populations epithelial-like, aldehyde dehydrogenase (ALDH)-expressing and mesenchymal-like, CD44+CD24-/low CSCs found significantly different...
To retrospectively assess the value of endorectal magnetic resonance (MR) imaging and MR spectroscopy combined with free-to-total prostate-specific antigen (PSA) ratio for detecting prostate cancer in men elevated PSA levels.The institutional review board approved study, all patients provided informed written consent. Endorectal were performed 54 levels greater than 3 ng/mL but less 15 20%, followed by sextant biopsy peripheral zone. For each patient, spectroscopic findings, level, analyzed...
Certain dietary interventions might improve the therapeutic index of cancer treatments. An alternative to "drug plus diet" approach is pharmacological reproduction metabolic traits such diets. Here we explored impact adding metformin an established regimen on systemic host metabolism patients. A panel 11 serum metabolites including markers mitochondrial function and intermediates/products folate-dependent one-carbon were measured in paired baseline post-treatment sera obtained from...
// Sílvia Cufí 1,2 , Bruna Corominas-Faja Eugeni Lopez-Bonet 3,14 Rosa Bonavia 4 Sonia Pernas 5,14 Isabel Álvarez López 6,14 Joan Dorca 2,7,14 Susana Martínez 8,14 Norberto Batista 9,14 Severina Domínguez Fernández 10,14 Elisabet Cuyàs Joana Visa Esther Rodríguez-Gallego 11 Quirantes-Piné 12 Antonio Segura-Carretero Jorge Joven Begoña Martin-Castillo 2,13,14 Javier A. Menendez 1,2,14 1 Metabolism & Cancer Group, Translational Research Laboratory, Catalan Institute of Oncology, Girona,...
We report the case of a 19-year-old woman who presented with hepatic mass without cirrhosis. Light microscopy revealed cholangiocarcinoma having both well-differentiated adenocarcinoma and lymphoepithelioma-like undifferentiated carcinoma components. By immunohistochemistry, tumor showed strong diffuse expression for cytokeratin AE1, 5D3, CK22. The cells were positive p53 protein (more than 75% cells) but negative bcl-2 LMP1. Abundant Epstein-Barr virus EBER (1/2) oligonucleotides detected...