A5009478706- CRISPR and Genetic Engineering
- Retinal Development and Disorders
- Zebrafish Biomedical Research Applications
- Cellular Mechanics and Interactions
- Single-cell and spatial transcriptomics
- Developmental Biology and Gene Regulation
- Hippo pathway signaling and YAP/TAZ
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- RNA modifications and cancer
Centro Andaluz de Biología del Desarrollo
2021-2024
Genetic studies in human and mice have established a dual role for Vsx genes retina development: an early function progenitors’ specification, later requirement bipolar-cells fate determination. Despite their conserved expression patterns, it is currently unclear to which extent functions are also across vertebrates, as mutant models available only mammals. To gain insight into vsx teleosts, we generated vsx1 vsx2 CRISPR/Cas9 double knockouts ( KO) zebrafish. Our electrophysiological...
Sight depends on the tight cooperation between photoreceptors and pigmented cells, which derive from common progenitors through bifurcation of a single gene regulatory network into neural retina (NR) retinal-pigmented epithelium (RPE) programs. Although genetic studies have identified upstream nodes controlling these networks, their logic remains poorly investigated. Here, we characterize transcriptome dynamics chromatin accessibility in segregating NR/RPE populations zebrafish. We analyze...
Abstract The assembly of the embryo’s primary axis is a fundamental landmark for establishment vertebrate body plan. Although morphogenetic movements directing cell convergence towards midline have been described extensively, little known on how gastrulating cells interpret mechanical cues. Yap proteins are well-known transcriptional mechanotransducers, yet their role in gastrulation remains elusive. Here we show that double knockout yap and its paralog yap1b medaka results an failure, due...
Developmental and physiological processes depend on the transcriptional translational activity of heterogeneous cell populations. A main challenge in gene expression studies is dealing with this intrinsic complexity while keeping sequencing efficiency. Translating ribosome affinity purification (TRAP) methods have allowed cell-specific recovery polyribosome-associated RNAs by genetic tagging ribosomes selected Here we combined TRAP approach adapted enhancer trap (trap-TRAP) to systematically...
The ontogeny of the vertebrate retina has been a topic interest to developmental biologists and human geneticists for many decades. Understanding unfolding genetic program that transforms field progenitors' cells into functionally complex multi‐layered sensory organ is formidable challenge. Although classical studies succeeded in identifying key regulators specification, we are far from understanding architecture their gene regulatory networks (GRNs) thus predicting behaviour. A goal our...
Abstract Genetic studies in human and mice have established a dual role for Vsx genes retina development: an early function progenitors’ specification, later requirement bipolar-cells fate determination. Despite their conserved expression patterns, it is currently unclear to which extent functions are also across vertebrates, as mutant models available only mammals. To gain insight into vsx teleosts, we generated vsx1 vsx2 CRISPR-Cas9 double knockouts ( vsxKO ) zebrafish. Our...
Abstract Developmental and physiological processes depend on the transcriptional translational activity of heterogeneous cell populations. A main challenge in gene expression studies is dealing with this intrinsic complexity while keeping sequencing efficiency. Translating ribosome affinity purification (TRAP) methods have allowed cell-specific recovery polyribosome-associated RNAs by genetic tagging ribosomes selected Here we combined TRAP approach adapted enhancer trap (trap-TRAP) to...
ABSTRACT The condensation of the embryo primary axis is a fundamental landmark in establishment vertebrate body plan. Although complex morphogenetic movements directing cell convergence towards midline have been described extensively, little known on how gastrulating cells interpret mechanical cues. Yap proteins are among best characterized transcriptional mechanotransducers, yet their role gastrulation has remained elusive. Here we show that double knockout yap and its paralog yap1b medaka...