A5031563436- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biosensors and Analytical Detection
- Electrowetting and Microfluidic Technologies
- Advanced biosensing and bioanalysis techniques
- Molecular Biology Techniques and Applications
- Analytical Chemistry and Chromatography
- Analytical Chemistry and Sensors
- Toxic Organic Pollutants Impact
- Glycosylation and Glycoproteins Research
- Advanced Biosensing Techniques and Applications
- 3D Printing in Biomedical Research
- Estrogen and related hormone effects
- Mass Spectrometry Techniques and Applications
- Forensic and Genetic Research
- Identification and Quantification in Food
- Neuroscience and Neuropharmacology Research
- Cell Image Analysis Techniques
- Nanopore and Nanochannel Transport Studies
- Electrohydrodynamics and Fluid Dynamics
- CRISPR and Genetic Engineering
- Advanced Chemical Sensor Technologies
- SARS-CoV-2 detection and testing
- Receptor Mechanisms and Signaling
University of Virginia
2016-2025
McCormick (United States)
2010-2022
American Academy of Forensic Sciences
2021
Institute of Forensic Science
2021
Boston University
2020
Charlottesville Medical Research
2013-2019
Occidental College
2015
University of Massachusetts Chan Medical School
2013
UMass Memorial Health Care
2013
University of Pittsburgh
1997-2010
ModesIntroduction to Capillary Electrophoresis, R.P. Oda and J.P. LandersMicellar Electrokinetic Chromatography, J.R. MazzeoCapillary Electrophoresis Separation of Enantiomers by Cyclodextrin Array Chiral Analysis, A. GuttmanCapillary Isoelectric Focusing, R. Rodr guez-D az, T. Wehr, M. Zhu, V. LeviTheory Practice Electrochromatography, M.M. Dittmann G.P. RozingAnalyteCapillary Ion W.R. JonesAnalysis Small Organic Molecules K.D. AltriaCapillary Peptides, van de Goor, Apffel, J. Chakel, W....
We describe a microfluidic genetic analysis system that represents previously undescribed integrated device capable of accepting whole blood as crude biological sample with the endpoint generation profile. Upon loading sample, glass carries out on-chip DNA purification and PCR-based amplification, followed by separation detection in manner allows for microliter samples to be screened infectious pathogens sample-in-answer-out results < 30 min. A single syringe pump delivers sample/reagents...
A microchip solid-phase extraction method for purification of DNA from biological samples, such as blood, is demonstrated. Silica beads were packed into glass microchips and the immobilized with sol-gel to provide a stable reproducible solid phase onto which could be adsorbed. Optimization loading conditions established higher recovery at pH 6.1 than 7.6. This lower also allowed flow rate increased, resulting in decrease time 25 min less 15 min. Using this procedure, template genomic human...
A silica-based solid-phase extraction system suitable for incorporation into a microchip platform (ν-total analytical system; ν-TAS) would find utility in variety of genetic analysis protocols, including DNA sequencing. The procedure utilized is based on adsorption the onto bare silica. involves three steps: (i) presence chaotropic salt, (ii) removal contaminants with an alcohol/water solution, and (iii) elution adsorbed small volume buffer polymerase chain reaction (PCR) amplification....
Efficient and stable quenching of electrochemiluminescence (ECL) tris(2,2‘-bipyridine)ruthenium(II) by oxidizing ferrocene methanol (FcMeOH) at the electrode is reported. Bimolecular energy or electron transfer between Ru(bpy)32+* ferrocenium (Fc+), oxidized species Fc, along with suppression radical reactions suggested as mechanism for ECL. Fc shows more efficient ECL compared known quenchers phenol 1,1-dimethyl-4,4‘-bipyridine dication (MV2+). The rate constant was 5.6 × 1010 M-1 s-1....
Unlike recent studies that have depended on manipulation of separation buffer parameters to facilitate stacking neutral analytes in micellar capillary electrophoresis (MCE) mode, we developed a method based simply the sample matrix. Many solutions for MCE are strict control pH, micelle type, electroosmotic flow (EOF) rate, and separation-mode polarity. However, universal solution should allow free without substantially affecting analytes. Analogous zone by invoking field amplification...
Interest in improving the speed of DNA analysis via capillary electrophoresis has led to efforts integrate amplification into microfabricated devices. This been difficult achieve since thermocycling required for effective polymerase chain reaction (PCR) is dependent on an contact between heating source and PCR mixture vessel. We describe a noncontact method rapid mixtures electrophoretic chip-like glass chambers. The mediated through use tungsten lamp as inexpensive infrared radiation...
As recently as the early 1990s, DNA purification was time-consuming, requiring use of toxic, hazardous reagents. The advent solid phase extraction techniques and availability commercial kits for quick reliable has relegated those largely to history books. High quality can now be extracted from whole blood, serum, saliva, urine, stool, cerebral spinal fluid, tissues, cells in less time without sacrificing recovery. Having achieved such a radical change methodology extraction, focus shifted...
A glass microdevice has been constructed for the on-line integration of solid-phase extraction (SPE) DNA and polymerase chain reaction (PCR) on a single chip. The chromatography required SPE in microfluidic sample preparation device (μSPD) was carried out silica bead/sol−gel bed, where purified eluted directly into downstream chamber conventional thermocycling allowed PCR amplification specific target sequences. Through rapid, simple passivation with silanizing reagent, reproducible...
A novel DNA solid-phase extraction protocol based on the pH-dependent charge of chitosan was developed specifically for low-volume microchips. The method uses chitosan-coated beads to extract at pH 5 and release it from 9. efficiency as high 92% could be attained, even complex samples such human blood containing significant amounts protein. Using this method, PCR inhibitors that are typically used in procedures (e.g., chaotropic salts, 2-propanol) can avoided, making more conducive...
Loop-mediated isothermal amplification (LAMP) as a diagnostic tool is rapidly gaining recognition and maturity. Among various advantages over traditional polymerase chain reaction, the ability to visually detect by incorporation of colorimetric indicators one its most unique features. There an overwhelming variety LAMP in literature, yet comprehensive comparative study lacking. This evaluates use hydroxynaphthol blue, phenol red, calcein, leuco crystal violet, malachite green, fluorescent...
ADVERTISEMENT RETURN TO ISSUEPREVReviewAdvances in Polymerase Chain Reaction on Microfluidic ChipsMichael G. Roper, Christopher J. Easley, and James P. LandersView Author Information Department of Chemistry, University Virginia, Charlottesville, Virginia 22904, Pathology, Health Science Center, 22908 Cite this: Anal. Chem. 2005, 77, 12, 3887–3894Publication Date (Web):May 19, 2005Publication History Published online19 May 2005Published inissue 1 June...
The extraction and amplification of DNA from biological samples is laborious time-consuming, requiring numerous instruments sample handling steps. An integrated, single-use, poly(methyl methacrylate) (PMMA) microdevice for would benefit clinical forensic communities, providing a completely closed system with rapid sample-in-PCR-product-out capability. Here, we show the design simple flow control required enzyme-based preparation PCR buccal swabs or liquid whole blood an ∼5-fold reduction in...