A5020086283- Heart Rate Variability and Autonomic Control
- Neuroscience of respiration and sleep
- Renin-Angiotensin System Studies
- Comparative Animal Anatomy Studies
- Blood Pressure and Hypertension Studies
- Hormonal Regulation and Hypertension
- Circadian rhythm and melatonin
- Sodium Intake and Health
- Eicosanoids and Hypertension Pharmacology
- Ichthyology and Marine Biology
- Nitric Oxide and Endothelin Effects
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Renal and Vascular Pathologies
- Neuroendocrine regulation and behavior
- Birth, Development, and Health
- Sleep and Wakefulness Research
- Biochemical effects in animals
- Cardiovascular Disease and Adiposity
- Apelin-related biomedical research
- Adipose Tissue and Metabolism
- Liver Disease and Transplantation
- Ion Transport and Channel Regulation
- Angiogenesis and VEGF in Cancer
- Estrogen and related hormone effects
- Stress Responses and Cortisol
Universidade Federal de São Paulo
2016-2025
Fundação de Apoio à Universidade Federal de São Paulo
2011-2023
Ministério da Saúde
2023
Beneficência Portuguesa de São Paulo
2023
Foie, Métabolisme, Cancer
2023
Universidade Federal do Rio Grande do Sul
2005-2020
Hamad Medical Corporation
2017
University of Cape Town
2017
South African Medical Research Council
2017
Mulago Hospital
2017
Most data on mortality and prognostic factors in patients with heart failure come from North America Europe, little information other regions. Here, the International Congestive Heart Failure (INTER-CHF) study, we aimed to measure at 1 year Africa, China, India, Middle East, southeast Asia South America; also explored demographic, clinical, socioeconomic variables associated mortality.We enrolled consecutive (3695 [66%] clinic outpatients, 2105 [34%] hospital patients) 108 centres six...
Based on previous data, we hypothesized that an increase of angiotensin II (Ang II)—via the Ang type 1 (AT-1) receptor—in rostral ventrolateral medulla (RVLM) and paraventricular nucleus (PVN) hypothalamus could activate NAD(P)H oxidase will produce superoxides resulting in increased sympathetic activity hypertension. The mRNA expression AT-1 receptors, subunits (p47phox gp91phox), CuZnSOD were analyzed RVLM PVN male Wistar rats (Goldblatt hypertension model, 2K-1C). In addition,...
Oxidative stress is a state in which excess reactive oxygen species (ROS) overwhelm endogenous antioxidant systems. It known that this has been involved the development of hypertension. On basis previous data, we hypothesized overactivity NAD(P)H oxidase-derived ROS and lowered activity CuZnSOD, an within rostral ventrolateral medulla (RVLM), could contribute to 2K-1C (two-kidney one-clip) Moreover, test functional significance whether oxidative was maintenance sympathetic vasomotor tone...
Reactive oxygen species in peripheral cardiovascular tissues are implicated the pathogenesis of 2 kidney-1 clip hypertension. We recently identified an imbalance between reactive generation and oxidant scavenging rostral ventrolateral medulla (RVLM) rats. tested whether enhanced superoxide signaling RVLM rats contributes to chronic hypertension via sympathetic activation conscious by overexpressing cytoplasmically targeted dismutase using adenoviral vector (Ad-CMV-CuZnSOD) Wistar (male, 150...
Renovascular hypertension induced by 2 Kidney-1 Clip (2K-1C) is a renin-angiotensin-system (RAS)-dependent model, leading to renal vascular rarefaction and failure. RAS inhibitors are not able reduce arterial pressure (AP) and/or preserve the function, thus, alternative therapies needed. Three weeks after left artery occlusion, fluorescently tagged mesenchymal stem cells (MSC) (2×105 cells/animal) were injected weekly into tail vein in 2K-1C hypertensive rats. Flow cytometry showed labeled...
Despite extensive use of the renovascular/Goldblatt model hypertension-2K-1C, and renal denervation to treat drug resistant hypertensive patients, autonomic mechanisms that underpin maintenance this hypertension are important yet remain unclear. Our aim was analyse cardiovascular function by power spectral density analysis both arterial pressure pulse interval measured continuously radio telemetry for 6weeks after artery clipping. Mean increased from 106±5 185±2mmHg during 5weeks post...
Renal nerve stimulation at a low frequency (below 2 Hz) causes water and sodium reabsorption via α 1 -adrenoreceptor tubular activation, process independent of changes in systemic blood pressure, renal flow, or glomerular filtration rate. However, the underlying mechanism is not fully understood. Since sympathetic nervous system intrarenal ANG II appear to act synergistically mediate reabsorption, we hypothesized that low-frequency acute electrical (ESRN) activates NHE3-mediated AT receptor...
Abstract The aim of the present study was to examine participation rostral ventrolateral medulla (RVLM) in maintenance hypertension rats submitted renovascular Goldblatt (two-kidney, one clip) procedure. We inhibited or stimulated this area with use drugs such as glycine, l -glutamate, kynurenic acid. (1) Bilateral microinjection glycine (100 nmol, 200 nL, n=13) into RVLM hypertensive produced a decrease mean arterial blood pressure (MAP) from 177.2±29.3 102.3±20.9 mm Hg ( P <.05), which...
We hypothesized that upregulation of angiotensin type 1 receptor (AT(1)R) and inducible nitric oxide (NO) synthase (iNOS) within the rostral ventrolateral medulla (RVLM) could contribute to two-kidney, one-clip (2K-1C) hypertension.The experiments were performed in male Wistar rats, 6 weeks after renal surgery. The animals divided into control (SHAM, n = 18) hypertensive groups (2K-1C, 18). Bilateral tissue punches taken from sections containing RVLM perform iNOS gene expression analyses by...
Abstract An imbalance of excitatory and inhibitory functions has been shown to contribute numerous pathological disorders. Accumulating evidence supports the idea that a change in hypothalamic γ‐aminobutyric acid (GABA)‐ergic glutamatergic synaptic contributes exacerbated neurohumoral drive prevalent cardiovascular disorders, including hypertension. However, precise underlying mechanisms neuronal substrates are still not fully elucidated. In present study, we combined quantitative...
Sympathetic vasomotor hyperactivity and baroreflex dysfunction are involved in the development maintenance of renovascular arterial hypertension. We hypothesized that angiotensin (Ang) II-dependent oxidative stress contributes to pathophysiology two-kidney, one-clip (2K-1C) model.The mean pressure (MAP), baroreflex, renal sympathetic nerve activity (rSNA) were evaluated after chronic administration an antioxidant, vitamin C (vitC 150 mg/kg/day) male Wistar 2K-1C rats. Additionally, mRNA...
The aim of this investigation was to evaluate genetic damage induced in male rats by experimental sleep loss for short-term (24 and 96 h) long-term (21 days) intervals, as well their respective recovery periods peripheral blood, brain, liver heart tissue the single cell gel (comet) assay. Rats were paradoxically deprived (PSD) platform technique 24 or h, chronically sleep-restricted (SR) 21 days. We also sought verify time course after h rebound sleep. results showed DNA blood cells...
Previous studies showed that the microinjection of antioxidants or overexpression superoxide dismutase within rostral ventrolateral medulla (RVLM) reduces hypertension and sympathoexcitation in 2-kidney, 1-clip (2K-1C) model. In this study, we hypothesized angiotensin II (ANG II) type 1 receptor (AT1R) is involved oxidative stress RVLM contributes to cardiovascular dysfunction renovascular hypertension. Losartan (30mg/kg/day, oral gavage) was administered for 7 consecutive days by week 5...
New Findings What is the central question of this study? The aim was to evaluate whether elevated sympathetic nerve activity (SNA) occurs before onset arterial hypertension in a rat renovascular model hypertension, two‐kidney, one‐clip (2K1C) Goldblatt model, and determine possible mechanisms origins. main finding its importance? Sympathetic can be raised by third week after renal artery clipping, originates, part, from enhanced respiratory modulation. Spinal circuits contribute elevation...
Abstract High glucose concentration in the airway surface liquid (ASL) is an important feature of diabetes that predisposes to respiratory infections. We investigated role alveolar epithelial SGLT1 activity on ASL and bacterial proliferation. Non-diabetic diabetic rats were intranasally treated with saline, isoproterenol (to increase activity) or phlorizin decrease activity); 2 hours later, proliferation (methicillin-resistant Sthaphylococcus aureus , MRSA Pseudomonas aeruginosa, P....
The Goldblatt model of hypertension (2K-1C) in rats is characterized by renal sympathetic nerve activity (rSNA). We investigated the effects unilateral denervation clipped kidney (DNX) on sodium transporters unclipped kidneys and cardiovascular, autonomic, functions 2K-1C control (CTR) rats. mean arterial pressure (MAP) rSNA were evaluated experimental groups. Kidney function NHE3, NCC, ENaCβ, ENaCγ protein expressions assessed. glomerular filtration rate (GRF) plasma flow not changed DNX,...