A5019977038- Regulation of Appetite and Obesity
- Hypothalamic control of reproductive hormones
- Neuropeptides and Animal Physiology
- Cannabis and Cannabinoid Research
- Neuroendocrine regulation and behavior
- Receptor Mechanisms and Signaling
- Sleep and Wakefulness Research
- Neuroscience and Neuropharmacology Research
- Biochemical Analysis and Sensing Techniques
- Neurotransmitter Receptor Influence on Behavior
- Stress Responses and Cortisol
- Estrogen and related hormone effects
- Diet, Metabolism, and Disease
- Adipose Tissue and Metabolism
- Pain Mechanisms and Treatments
- Ion Channels and Receptors
- Neuroscience of respiration and sleep
- GABA and Rice Research
- Spine and Intervertebral Disc Pathology
- Biochemical effects in animals
- Spinal Fractures and Fixation Techniques
- Medical Imaging and Analysis
- Menstrual Health and Disorders
- Pharmacological Receptor Mechanisms and Effects
- Diet and metabolism studies
Western University of Health Sciences
2016-2025
Institute of Biomedical Science
2016
Philadelphia College of Osteopathic Medicine
2013
Oregon Health & Science University
1996-2002
Vollum Institute
1996
Michigan State University
1992-1994
The hypothalamus is a key region of the central nervous system involved in control homeostasis, including energy and core body temperature (Tc). 17β-Estradiol (E2) regulates Tc, part, via actions basal preoptic area. E2 primarily controls hypothalamic functions nuclear steroid receptors, estrogen receptor α/β. However, we have previously described an E2-responsive, Gq-coupled membrane that reduces postsynaptic inhibitory γ-aminobutyric acid-ergic tone attenuates postovariectomy weight gain...
Besides its quintessential role in reproduction, 17β-estradiol (E 2 ) is a potent anorexigenic hormone. E and the selective G q -coupled membrane estrogen receptor (Gq-mER) ligand STX rapidly increase excitability proopiomelanocortin (POMC) neurons by desensitizing coupling of GABA B receptors to protein-coupled inwardly rectifying K + channels (GIRKs), which upon activation elicit hyperpolarizing outward current. However, it unknown whether can modulate signaling neuropeptide Y...
Orphanin FQ (OFQ) is a novel heptadecapeptide whose structure resembles that of dynorphin A<sub>1–17</sub>. Its receptor shares appreciable homology with µ-, δ- and ĸ-opioid receptors, highly expressed in the hypothalamus. The present study examined effects OFQ on neurons within arcuate nucleus (ARC) mediobasal hypothalamus, using intracellular recordings from coronal slices. In current clamp, produced hyperpolarization ARC neurons, including those immunopositive for β-endorphin,...
GABA is a predominant neurotransmitter in the hypothalamus and an important regulator of hypothalamic function. To elucidate cellular basis for GABAergic action this region, we used intracellular recordings from identified neurons. Ninety-three percent mediobasal neurons responded to GABAB receptor stimulation, presence bicuculline-sensitive synaptic potentials indicated tonic, GABAA receptor-mediated input. Stimulation receptors hyperpolarized these cells by activating inwardly rectifying...
Insulin resistance is at the core of metabolic syndrome, and men exhibit a higher incidence syndrome than women in early adult life, but this sex advantage diminishes sharply when reach postmenopausal state. Because 17β-estradiol (E2) augments excitability anorexigenic proopiomelanocortin (POMC) neurons, we investigated neuroprotective effects E2 against insulin POMC neurons from diet-induced obese (DIO) female male mice. The efficacy to activate canonical transient receptor potential 5...
The principal role of estrogen is its control the female ovulatory cycle via negative and positive feedback on gonadotropin secretion. However, a detailed, cohesive picture how steroid specifically regulates excitability hypothalamic neurons involved in central secretion still emerging. Here, we used an ovariectomized guinea pig model to test hypothesis that acts GABAergic preoptic area (POA) elicit biphasic profile luteinizing hormone (LH) Intracellular electrophysiological recordings...
We sought to determine whether sex differences exist for the cannabinoid modulation of appetite, body temperature and neurotransmission at pro-opiomelanocortin (POMC) synapses. Gonadectomized male female guinea pigs were outfitted monitor core injected with either CB1 receptor agonist WIN 55,212-2 (1 mg/kg s.c.), antagonist AM251 (3 s.c.) or vehicle ml/kg evaluated changes in six indices feeding behavior under ad libitum conditions 7 days. elicited an overt, sexually differentiated...
We examined the neuroanatomical substrates and signaling mechanisms underlying suppressive effect of GLP1 on homeostatic hedonic feeding. Electrophysiological behavioral studies were conducted in agouti-related peptide (AgRP)-cre tyrosine hydroxylase (TH)-cre mice, AgRP-cre/pituitary adenylyl cyclase-activating polypeptide (PACAP) type I receptor (PAC1R)fl/fl animals. (30 pmol) delivered directly into arcuate nucleus (ARC) decreased feeding diminished rate consumption. This anorexigenic was...
Orexigenic mediators can impact the hypothalamic feeding circuitry via activation of AMP-dependent protein kinase (AMPK). Given that testosterone is an orexigenic hormone, we hypothesized androgenic changes in energy balance are due to enhanced cannabinoid-induced inhibition anorexigenic proopiomelanocortin (POMC) neurons AMPK. To this end, whole animal experiments were carried out gonadectomized male guinea pigs treated subcutaneously with either propionate (TP; 400 μg) or its sesame oil...
Testosterone exerts profound effects on reproduction and energy homeostasis. Like other orexigenic hormones, it increases endocannabinoid tone within the hypothalamic feeding circuitry. Therefore, we tested hypothesis that testosterone upregulates expression of diacylglycerol lipase (DAGL)α in arcuate nucleus (ARC) to increase intake via enhanced endocannabinoid-mediated retrograde inhibition anorexigenic proopiomelanocortin (POMC) neurons. Energy intake, meal patterns, expenditure were...
The present study sought to determine whether cannabinoids inhibit glutamatergic and GABAergic synaptic input onto neurons of the hypothalamic arcuate nucleus (ARC), estrogen modulates this process. Whole-cell patch clamp recordings were performed in slices prepared from ovariectomized female guinea pigs. CB1 receptor activation reduced amplitude excitatory postsynaptic currents (EPSCs) evoked by electrical stimulation that sensitive ionotropic glutamate antagonists. antagonist AM251...
Neurobiological mechanisms involved in sexual differentiation of the central nervous system will be presented with a comparative view across vertebrates. Women and men differ wide variety behavioral traits probabilities developing certain mental disorders. A brief overview sex-chromosome pathways underlying dimorphisms provided. We describe most common brain phenotypes derived vivo magnetic resonance imaging, discuss challenges interpreting these vis-à-vis neurobiology revise known sex...