A5067877995- Microtubule and mitosis dynamics
- Nuclear Structure and Function
- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
- Muscle Physiology and Disorders
- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Adipose Tissue and Metabolism
- Retinoids in leukemia and cellular processes
- Diabetic Foot Ulcer Assessment and Management
- Nerve injury and regeneration
- Plant nutrient uptake and metabolism
- Telomeres, Telomerase, and Senescence
- Cell Adhesion Molecules Research
Max Perutz Labs
2025
Vienna Biocenter
2025
University of Vienna
2025
Université Paris Cité
2020-2024
Institut Jacques Monod
2020-2024
Centre National de la Recherche Scientifique
2020-2024
La Ligue Contre le Cancer
2023
Center for Research and Advanced Studies of the National Polytechnic Institute
2017-2019
Instituto Politécnico Nacional
2019
Abstract Polo-like kinase 1 (Plk1) is instrumental for mitotic entry and progression. Plk1 activated by phosphorylation on a conserved residue Thr210 in its activation segment the Aurora A (AURKA), reaction that critically requires co-factor Bora phosphorylated CyclinA/B-Cdk1 kinase. Here we show phospho-Bora direct activator of AURKA activity. We localize key determinants function to 100 amino acid region encompassing two short Tpx2-like motifs phosphoSerine-Proline motif at Serine 112,...
Life of sexually reproducing organisms starts with the fusion haploid egg and sperm gametes to form genome a new diploid organism. Using newly fertilized Caenorhabditis elegans zygote, we show that mitotic Polo-like kinase PLK-1 phosphorylates lamin LMN-1 promote timely lamina disassembly subsequent merging parental genomes into single nucleus after mitosis. Expression non-phosphorylatable versions LMN-1, which affect depolymerization during mitosis, is sufficient prevent mixing chromosomes...
In this issue, El Mossadeq et al. (https://doi.org/10.1083/jcb.202403125) report that a structure forms around segregating chromosomes following meiosis I shares features with the nuclear envelope in interphase but also has distinct, unique characteristics.
Abstract The study of Hutchinson–Gilford progeria syndrome (HGPS) has provided important clues to decipher mechanisms underlying aging. Progerin, a mutant lamin A, disrupts nuclear envelope structure/function, with further impairment multiple processes that culminate in senescence. Here, we demonstrate the protein export pathway is exacerbated HGPS, due progerin‐driven overexpression CRM1, thereby disturbing nucleocytoplasmic partitioning CRM1‐target proteins. Enhanced central since...
The nuclear envelope, which protects and organizes the genome, is dismantled during mitosis. In Caenorhabditis elegans zygote, envelope breakdown (NEBD) of parental pronuclei spatially temporally regulated mitosis to promote unification maternal paternal genomes. Nuclear pore complex (NPC) disassembly a decisive step NEBD, essential for permeabilization. By combining live imaging, biochemistry, phosphoproteomics, we show that NPC stepwise process involves Polo-like kinase 1 (PLK-1)-dependent...
β-Dystroglycan (β-DG) is a transmembrane protein with critical roles in cell adhesion, cytoskeleton remodeling and nuclear architecture. This functional diversity attributed to the ability of β-DG target to, conform specific assemblies at plasma membrane (PM) envelope (NE). Although classical NLS importin α/β mediated import pathway has already been described for β-DG, intracellular trafficking route by which reaches nucleus unknown. In this study, we demonstrated that undergoes retrograde...
Previously, we reported that the Polo-like kinase PLK-1 phosphorylates single Caenorhabditis elegans lamin (LMN-1) to trigger lamina depolymerization during mitosis. We showed this event is required form a pronuclear envelope scission removes membranes on juxtaposed oocyte and sperm envelopes in zygote, allowing parental chromosomes merge nucleus after segregation (Velez-Aguilera et al., 2020). Here, show cortical microtubule pulling forces contribute by promoting mitotic spindle elongation,...
The nuclear envelope, which protects and organizes the interphase genome, is dismantled during mitosis. In C. elegans zygote, envelope breakdown (NEBD) of parental pronuclei spatially temporally regulated mitosis to promote unification genomes. During NEBD, Nuclear Pore Complex (NPC) disassembly critical for rupturing permeability barrier removing NPCs from membranes near centrosomes between juxtaposed pronuclei. By combining live imaging, biochemistry, phosphoproteomics, we characterized...
Abstract β-dystroglycan (β-DG) is a key component of multiprotein complexes in the plasma membrane and nuclear envelope. In addition, β-DG undergoes two successive proteolytic cleavages that result liberation its intracellular domain (ICD) into cytosol nucleus. However, stimuli-inducing ICD cleavage physiological relevance this fragment are largely unknown. study we show for first time targeted to nucleolus where it interacts with proteins B23 UBF (central factor Pol I-mediated rRNA gene...
Abstract Previously, we reported that the Polo-like kinase PLK-1 phosphorylates single C. elegans lamin (LMN-1) to trigger lamina depolymerization during mitosis. We showed this event is required for formation of a pronuclear envelopes scission removes membranes on juxtaposed oocyte and sperm in zygote, allowing parental chromosomes merge nucleus after segregation (Velez-Aguilera, 2020). Here show cortical microtubule pulling forces contribute by promoting mitotic spindle elongation. also...