A5019933883- Lipid Membrane Structure and Behavior
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Inflammatory Bowel Disease
- Microscopic Colitis
- Bacterial biofilms and quorum sensing
- Biochemical and Structural Characterization
- Carbohydrate Chemistry and Synthesis
- Liver Diseases and Immunity
- Antimicrobial Peptides and Activities
- Cellular transport and secretion
- Bacteriophages and microbial interactions
- Barrier Structure and Function Studies
- Epigenetics and DNA Methylation
- Supramolecular Self-Assembly in Materials
- T-cell and B-cell Immunology
- Gastrointestinal disorders and treatments
- Diverticular Disease and Complications
- Complement system in diseases
- Mass Spectrometry Techniques and Applications
- Photoreceptor and optogenetics research
- Caveolin-1 and cellular processes
- Surfactants and Colloidal Systems
- Autophagy in Disease and Therapy
- Cancer-related gene regulation
Dr Falk Pharma (Germany)
2020-2023
University of Freiburg
2015-2022
Faculty (United Kingdom)
2018
Pseudomonas aeruginosa is a ubiquitous environmental bacterium that one of the leading causes nosocomial infections. P. able to switch between planktonic, intracellular, and biofilm-based lifestyles, which allows it evade immune system as well antibiotic treatment. Hence, alternatives treatment are urgently required combat Lectins, like fucose-specific LecB, promising targets, because removal LecB resulted in decreased virulence mouse models. Currently, several research groups developing...
Cell adhesion is a crucial feature of all multicellular organisms, as it allows cells to organise themselves into tissues carry out specific functions. Here, we present mimetic approach that uses multivalent lectins with opposing binding sites crosslink glycan-functionalised giant unilamellar vesicles. The crosslinking process drives the progression from contact puncta elongated protocellular junctions, which form vesicles polygonal clusters resembling tissues. Due their carbohydrate...
Fucose-specific bacterial lectins bind to and stimulate mouse B cells, inducing an immune response in vivo.
Over the past decade, treatment targets for ulcerative colitis (UC) have become more stringent, incorporating multiple parameters. Recently, concept of 'disease clearance'-defined as combined clinical, endoscopic, and histological remission-has been proposed an ultimate endpoint in treating UC.To determine rates disease clearance patients with mild-to-moderate UC treated different doses mesalazine granules induction therapy.In a post hoc analysis, data were pooled from four randomised,...
The double-faced Janus lectin, designed by assembling sialic acid and fucose-specific organize multivalent heteroglyco compounds in mulitlayered material, glycosylated protocells prototissues.
Abstract The major feature of leukemic cells is an arrest differentiation accompanied by highly active proliferation. In many subtypes acute myeloid leukemia, these features are mediated the aberrant Wnt/ β -Catenin pathway. our study, we established lectin LecB as inducer leukemia cell line THP-1 and used it for investigation involved processes. During differentiation, functional autophagy low levels were essential. Corresponding to this, a high level stabilized proliferation inhibited...
Synthetic minimal membrane systems are extremely useful for better understanding of complex cellular structures and cell surface processes. We have developed a facile method synthesis cholesterylated peptides, each bearing carbohydrate moiety fluorescent tag. The position the cholesterol on peptide can be controlled by using new Fmoc-protected cholesterol-triazole-lysine group, which we constructed means solid-phase synthesis. succeeded in integrating glyco modules into giant unilamellar...
Membrane fusion is essential for the transport of macromolecules and viruses across membranes. While glycan-binding proteins (lectins) often initiate cellular adhesion, subsequent events require additional protein machinery. No mechanism membrane arising from simply a binding to glycolipids has been described thus far. Herein, we report that biotinylated derived cholera toxin becomes fusogenic lectin upon cross-linking with streptavidin. This novel reengineered brings about hemifusion...
The opportunistic bacterium Pseudomonas aeruginosa can infect mucosal tissues of the human body. To persist at barrier, this highly adaptable pathogen has evolved many strategies, including invasion host cells. Here, we show that P. lectin LecB binds and cross-links fucosylated receptors apical plasma membrane epithelial This triggers a signaling cascade via Src kinases phosphoinositide 3-kinase (PI3K), leading to formation patches enriched with basolateral marker phosphatidylinositol...
Abstract B cell superantigens crosslink conserved domains of receptors (BCRs) and cause dysregulated, polyclonal activation irrespective normal BCR-antigen complementarity. The cells typically succumb to activation-induced death, which can impede the adaptive immune response favor infection. In present study, we demonstrate that fucose-binding lectin Burkholderia ambifaria , BambL, bears functional resemblance superantigens. By engaging surface glycans, bacterial activated human peripheral...
Background Antibiotic treatment in acute uncomplicated diverticulitis (AUD) is commonplace; however, recent studies have questioned their therapeutic efficacy and placebo-controlled are scarce. Aims A trial was conducted to study the safety of broad-spectrum antibiotic Rifamycin SV multi-matrix (MMX)® (RIF-MMX) colon-specific formulation vs placebo. Methods phase 2 201 patients with AUD randomised 2:2:1 receive oral RIF-MMX 400 mg twice daily (BID) (RIF-800), 600 three times (RIF-1800) or...
Background In patients with mesalazine‐refractory ulcerative colitis, systemic corticosteroids are the treatment of choice. Objective To evaluate efficacy and safety prolonged release budesonide granules for induction remission in colitis. Methods Patients colitis discontinued mesalazine at baseline received 9 mg daily 8 weeks this open‐label, phase IIa study, followed by a 2‐week follow‐up wherein continued on alternate days (EudraCT number 2014‐005635‐14; ClinicalTrials.gov identifier...
Membrane fusion is essential for the transport of macromolecules and viruses across membranes. While glycan-binding proteins (lectins) often initiate cellular adhesion, subsequent events require additional protein machinery. No mechanism membrane arising from simply a binding to glycolipids has been described thus far. Herein we report that biotinylated derived cholera toxin, becomes fusogenic lectin upon crosslinking with streptavidin. This novel reengineered brings about hemifusion...
Abstract The opportunistic bacterium Pseudomonas aeruginosa produces the fucose-specific lectin LecB, which has been identified as virulence factor. LecB a tetrameric structure with four opposing binding sites and shown to act crosslinker. Here, we demonstrate that strongly binds glycosylated moieties of β1-integrins on basolateral plasma membrane epithelial cells caused rapid integrin endocytosis. Whereas internalized integrins were degraded via lysosomal pathway, washout restored cell...
Abstract The opportunistic bacterium Pseudomonas aeruginosa can infect mucosal tissues of the human body. To persist at barrier, this highly adaptable pathogen has evolved many strategies, including invasion host cells. Here, we show that P. lectin LecB binds and cross-links fucosylated receptors apical plasma membrane epithelial This triggers a signaling cascade via Src kinases PI3K leading to formation patches enriched with basolateral marker PIP 3 membrane. identifies as causative...
Membrane fusion is essential for the transport of macromolecules and viruses across membranes. While glycan-binding proteins (lectins) often initiate cellular adhesion, subsequent events require additional protein machinery. No mechanism membrane arising from simply a binding to glycolipids has been described thus far. Herein we report that biotinylated derived cholera toxin, becomes fusogenic lectin upon crosslinking with streptavidin. This novel reengineered brings about hemifusion...