A5100704497- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Electrospun Nanofibers in Biomedical Applications
- Food composition and properties
- Fungal and yeast genetics research
- Enzyme-mediated dye degradation
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Plant biochemistry and biosynthesis
- Microbial Metabolism and Applications
- biodegradable polymer synthesis and properties
- Nanocomposite Films for Food Packaging
- Lignin and Wood Chemistry
- Transgenic Plants and Applications
- Conducting polymers and applications
- Electrochemical sensors and biosensors
- Microbial Metabolites in Food Biotechnology
- Advanced Cellulose Research Studies
- Polysaccharides and Plant Cell Walls
- Endoplasmic Reticulum Stress and Disease
- Microbial Natural Products and Biosynthesis
- Fermentation and Sensory Analysis
- Probiotics and Fermented Foods
- Microplastics and Plastic Pollution
- Biochemical and Structural Characterization
South China University of Technology
2013-2025
Bioscience (China)
2008-2016
Abstract Background Komagataella phaffii , a type of methanotrophic yeast, can use methanol, favorable non-sugar substrate in eco-friendly bio-manufacturing. The dissimilation pathway K. leads to the loss carbon atoms form CO 2 . However, ΔFLD strain, engineered lack formaldehyde dehydrogenase—an essential enzyme pathway—displayed growth defects when exposed methanol-containing medium. Results Inhibiting triggers an excessive accumulation and decline intracellular NAD + /NADH ratio. Here, we...
Xylanase, an enzyme capable of hydrolyzing non-starch polysaccharides found in grain structures like wheat, has been to improve the organizational structure dough and thus increase its volume. In our past work, one promising xylanase FXYL derived from
The presence of arabinoxylan in wholewheat flour affects its quality significantly. Here, an efficient hydrolytic enzyme, Hmxyn, from Halolactibacillus miurensis was identified and heterologously expressed pichia pastoris. Moreover, relevant properties, including potential application the bread were evaluated. Recombinant Hmxyn exhibited maximal activity at 45°C pH 6.5, stable mid-range temperature (<55°C) (5.5-8.0) conditions. had a clear hydrolysis effect on wheat dough caused degradation...
Enzymes are highly efficient natural catalysts widely used in green biocatalysis, chemical, and pharmaceutical industries. However, their industrial applications often limited by high costs, poor stability, low activity....
Abstract Pectin lyase (PMGL) is an industrially important enzyme with widespread applications in the food, paper, and textile industries, owing to its capacity for direct degradation of highly esterified pectin. In this study, PMGL-Ba derived from Bacillus licheniformis underwent mining heterologous expression P. pastoris. Furthermore, diverse strategies, encompassing optimization cassette components, elevation gene dosage, co-expression chaperone factors, were employed augment production...
Abstract Machine learning (ML) constructs predictive models by understanding the relationship between protein sequences and their functions, enabling efficient identification of with high fitness values without falling into local optima, like directional evolution. However, how to extract most pertinent functional feature information from a limited number is vital for optimizing performance ML models. Here, we propose scut_ProFP (Protein Fitness Predictor), framework that integrates...
The quality deterioration that is induced by freezing treatment limits the development of frozen dough technology for standardized and delayed baking. In this study, laccase (LAC) ferulic acid (FA) were employed to improve rheological properties microstructure unfermented dough. results showed with LAC + FA had a lower softening degree than alone. Correspondingly, incorporation enhanced viscoelastic behavior better stability. Furthermore, more uniform homogeneous gluten network was observed...
For improving probiotics' survivability under harsh conditions, this study used Lactiplantibacillus plantarum GIM1.648 as a model microorganism to investigate its ability produce biofilms on electrospun ethyl cellulose nanofiber mats. SEM observations confirmed that biofilm was successfully formed the nanofibers, with latter being an excellent scaffold material. The optimal cultivation conditions for formation were MRS medium without Tween 80, culture time of 36 h, temperature 30 °C, pH 6.5,...
The production of heterologous proteins via microbial secretion is currently a prominent focus in biological product development. However, the full realization this method remains hindered by limited understanding protein mechanism with target microorganisms. To enhance production, chassis cell are being reinforced synthetic biology and metabolic engineering, proving highly efficient. Among microorganisms, Gram-positive bacteria widely employed due to their absence an outer membrane,...
Abstract The efficiency and effects of enzymatic deinking using xylanase lipase mixed with amylase (XLA) for secondary fibers were investigated office waste (MOW), photocopy prints (PHOT) old newspapers (ONP). optical physical properties deinked pulps evaluated. Changes in fiber morphology, crystallinity index functional groups also monitored. results show that non-impact (MOW PHOT), a maximum 19.9 % 13.9 obtained (XLA), respectively. For ONP, conventional alkaline demonstrated the highest...
A novel nano/micro-structured pesticide detection card was developed by combining electrospinning and hydrophilic modification, its feasibility for detecting different pesticides investigated. Here, the plain hydrophilic-modified poly(ε-caprolactone) (PCL) fiber mats were used absorption of indolyl acetate acetylcholinesterase (AChE), respectively. By pre-treating mat with ethanol, surface wettability improved, thus, promoting hydrolysis PCL mat. Furthermore, efficiency AChE improved almost...