A5061400918- Mechanical Circulatory Support Devices
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Cardiac Structural Anomalies and Repair
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- CAR-T cell therapy research
- Cardiac Fibrosis and Remodeling
- Chemotherapy-induced cardiotoxicity and mitigation
- Cardiac Arrest and Resuscitation
- Asthma and respiratory diseases
- Cardiac pacing and defibrillation studies
- Congenital Heart Disease Studies
- Neuroscience and Neural Engineering
- Cardiac Ischemia and Reperfusion
- Pharmacological Effects and Assays
- Cardiovascular Function and Risk Factors
- Cardiac and Coronary Surgery Techniques
- Cardiac Arrhythmias and Treatments
- Inhalation and Respiratory Drug Delivery
- Aortic Disease and Treatment Approaches
Center for Devices and Radiological Health
2022-2023
United States Food and Drug Administration
2023
Office of Science
2023
University of Louisville
2018-2022
University of Louisville Hospital
2022
Flinders Medical Centre
1987
Abstract There is need for a reliable in vitro system that can accurately replicate the cardiac physiological environment drug testing. The limited availability of human heart tissue culture systems has led to inaccurate interpretations cardiac-related effects. Here, we developed c ardiac t issue ulture m odel (CTCM) electro-mechanically stimulate slices with stretches systole and diastole during cycle. After 12 days culture, this approach partially improved viability but did not completely...
Objective: Continuous Flow Left Ventricular Assist Devices (CFLVAD) are circulatory support devices that implanted in patients with end-stage heart failure. We developed a novel control algorithm for CFLVAD to maintain physiologic perfusion while avoiding ventricular suction using only the intrinsic pump measurement of speed and without utilizing model-based estimation. Methods: The controller objective is differential setpoint. A mathematical model system coupled was used test silico....
Many novel drugs fail in clinical studies due to cardiotoxic side effects as the currently available vitro assays and vivo animal models poorly predict human cardiac liabilities, posing a multi-billion-dollar burden on pharmaceutical industry. Hence, there is worldwide unmet medical need for better approaches identify drug cardiotoxicity before undertaking costly time consuming 'first man' trials. Currently, only immature cells (human induced pluripotent stem cell-derived cardiomyocytes...
Continuous flow ventricular assist device (CFVAD) support in advanced heart failure patients causes diminished pulsatility, which has been associated with adverse events including gastrointestinal bleeding, end organ failure, and arteriovenous malformation. Recently, pulsatility augmentation by pump speed modulation proposed as a means to minimize events. Pulsatility primarily affects endothelial smooth muscle cells the vasculature. To study effects of pulse using CFVADs, we have developed...
Many novel drugs fail in clinical studies due to cardiotoxic side effects as the currently available vitro assays and vivo animal models poorly predict human cardiac liabilities, posing a multi-billion-dollar burden on pharmaceutical industry. Hence, there is worldwide unmet medical need for better approaches identify drug cardiotoxicity before undertaking costly time consuming 'first man' trials. Currently, only immature cells (human induced pluripotent stem cell-derived cardiomyocytes...
Mechanical circulatory support (MCS) device therapy is one of the primary treatment options for end-stage heart failure (HF), whereby a mechanical pump integrated with failing to maintain adequate tissue perfusion. The ISO 14708-5:2020 standard prescribes generic guidelines nonclinical evaluation and system performance testing MCS devices using mock loop (MCL). However, utility MCLs in premarket regulatory submissions ambiguous, specific disease states that intended treat are not usually...
Abstract The limited availability of human heart tissue and its complex cell composition are major limiting factors for reliable testing drug efficacy, toxicity understanding mechanism. Recently, we developed a functional pig slice biomimetic culture system that fully preserves the viability functionality 300 µm slices 6 days. Here, tested reliability this in delineating mechanisms known anti-cancer drugs cause cardiomyopathy. We three (doxorubicin, trastuzumab, sunitinib) associated with...
Continuous flow right ventricular assist devices are mechanical circulatory support that implanted in patients with end-stage heart failure. Right-sided failure often occurs conjunction the left-sided or can occur on its own as a result of attacks, dilated and hypertrophic cardiomyopathy valvular diseases. Here, we propose control algorithm for continuous maintain physiologic perfusion avoid suction under various physical conditions using only intrinsic pump speed. The maintains differential...
Abstract Background: There is an urgent need for a reliable in vitro system that can accurately replicate the cardiac physiological environment drug testing. The limited availability of human heart tissue culture systems has led to inaccurate interpretations related effects. Our group had previously developed pig/human slices enables functional and structural viability 6 days under electric stimulation enriched media reliably demonstrate subacute toxic effects anti-cancer therapeutics....
SUMMARY 1. Sixteen stable asthmatics had the protective effects of inhaled fenoterol (200 μg) and ipratropium bromide (60 against standardized histamine inhalation tests at 1 h examined in a randomized double blind fashion. 2. There was no significant difference baseline forced expired volume s (FEV ) for two study days ( P >0.05). 3. an increase FEV, on day compared with (0.26 versus 0.17 1; < 0.05). 4. The geometric mean provocative concentration to cause 20% fall (PC 20 6.31 mg/ml...
Purpose of Study: Many current VAD technologies incorporate artificial pulsatility to broadly mimic the dynamic nature blood flow and potentially reduce pump thrombus. However, true benefits drawbacks on patients' hemodynamics remain largely unknown. This study aims characterize clinically relevant pulsatile waveforms generated bench, assess their effects hemodynamics, establish standard test methods for evaluating VADs with pulsatility. Methods: An in-house programmable centrifugal was used...
Introduction: There is an urgent need for a reliable in vitro system to accurately replicate the cardiac physiological and pathological environment drug testing. The limited availability of human heart tissue culture systems has led inaccurate interpretations cardiac-related effects. Our group developed pig/human slices that enables functional structural viability 6 days using continuous electrical stimulation enriched media. This can more reliably detect cardiotoxic effects compared...
Introduction: Drug induced cardiotoxicity is a major cause of market withdrawal. The limited availability human heart tissue has led to inaccurate interpretations cardiac related drug effects. Our group had developed culture system for pig/human slices that functionally and structurally viable 6 days under electric stimulation enriched media could reliably demonstrate the subacute toxic effect anti-cancer therapeutics. However, by day 10, underwent cardiomyocyte dedifferentiation fibrotic...