A5080225610- Plant Molecular Biology Research
- Antioxidant Activity and Oxidative Stress
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Light effects on plants
- Cellular transport and secretion
- Postharvest Quality and Shelf Life Management
- Endoplasmic Reticulum Stress and Disease
- Genomics and Rare Diseases
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Lipid metabolism and biosynthesis
- Plant Physiology and Cultivation Studies
- Plant-Microbe Interactions and Immunity
- Microbial Metabolic Engineering and Bioproduction
- Genetic Mapping and Diversity in Plants and Animals
- Chromatin Remodeling and Cancer
- Renin-Angiotensin System Studies
- Polysaccharides and Plant Cell Walls
- Microbial Metabolites in Food Biotechnology
- Plant Micronutrient Interactions and Effects
- Phosphodiesterase function and regulation
- Plant Reproductive Biology
Instituto de Agrobiotecnología del Litoral
2020-2022
University of Buenos Aires
2015-2022
Consejo Nacional de Investigaciones Científicas y Técnicas
2010-2022
Instituto de Biotecnología y Biología Molecular
2022
National Agricultural Technology Institute
2010-2020
Instituto Nacional de Tecnologia
2011-2020
National Institute of Industrial Technology
2010-2015
Universidade de São Paulo
2008-2013
Instituto de Botânica
2010-2013
Yale University
2013
Vegetables are critical for human health as they a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis VTE compounds, tocopherol and tocotrienol, derives from precursors shikimate methylerythritol phosphate pathways. Quantitative trait loci (QTL) α-tocopherol content in ripe fruit have previously been determined an Solanum pennellii tomato introgression line population. this work, variations isoforms (α, β, γ, δ) fruits these lines were studied. parallel all genes...
With the aim of determining genetic basis metabolic regulation in tomato fruit, we constructed a detailed physical map genomic regions spanning previously described quantitative trait loci Solanum pennellii introgression line population. Two libraries from S. were screened with 104 colocated markers five selected regions, and total 614 bacterial artificial chromosome (BAC)/cosmids identified as seed clones. Integration sequence data maps lycopersicum facilitated anchoring 374 these...
Abstract Since the advent of postgenomic era, efforts have focused on development rapid strategies for annotating plant genes unknown function. Given its simplicity and rapidity, virus-induced gene silencing (VIGS) has become one preeminent approaches functional analyses. However, several problems remain intrinsic to use such a strategy in study both metabolic developmental processes. The most prominent these is commonly observed phenomenon “sectoring” tissue regions that are not effectively...
In tomato, numerous wild-related species have been demonstrated to be untapped sources of valuable genetic variability, including pathogen-resistance genes, nutritional, and industrial quality traits. From a collection S. pennellii introgressed lines, 889 fruit metabolic loci (QML) 326 yield-associated (YAL), distributed across the tomato genome, had identified previously. By using combination molecular marker sequence analysis, PCR amplification sequencing, analysis allelic variation,...
Pectin is a main component of the plant cell wall and most complex family polysaccharides in nature. Its composition essential for normal growth morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role physiology, tomato, pectin structure contributes to very important quality traits such fruit firmness. Sixty-seven different enzymatic activities have been suggested be required biosynthesis, but only few genes identified studied so far. This study...
Changes in environmental temperature influence many aspects of plant metabolism; however, the underlying regulatory mechanisms remain poorly understood. In addition to their role light perception, phytochromes (PHYs) have been recently recognized as sensors affecting growth. particular, Arabidopsis (Arabidopsis thaliana), high reversibly inactivates PHYB, reducing photomorphogenesis-dependent responses. Here, we show phytochrome-dependent perception modulating accumulation isoprenoid-derived...
Recent genetic studies have linked mental illness to alterations in disrupted schizophrenia 1 (DISC1), a multifunctional scaffolding protein that regulates cyclic adenosine monophosphate (cAMP) signaling via interactions with phosphodiesterase 4 (PDE4). High levels of cAMP during stress exposure impair function the prefrontal cortex (PFC), region gravely afflicted illness. As can aggravate illness, insults DISC1 may worsen symptoms by increasing levels. The current study examined whether...
Abstract Sucrose metabolism is important for most plants, both as the main source of carbon and via signaling mechanisms that have been proposed this molecule. A cleaving enzyme, invertase (INV) channels sucrose into sink metabolism. Although acid soluble insoluble invertases largely investigated, studies on role neutral (A/N-INV) lagged behind. Here, we identified a tomato A/N-INV encoding gene (NI6) co-localizing with previously reported quantitative trait locus (QTL) affecting primary in...
Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of plant cells and essential human diet. Both intermediates final products tocopherol biosynthetic pathway must cross plastid membranes to reach their sites action. So far, no protein with binding activity has been reported plants. Here, we demonstrated that tomato SlTBP is targeted chloroplasts able bind α-tocopherol. SlTBP-knockdown plants exhibited reduced levels both leaves fruits....
Limitations in our understanding about the mechanisms that underlie source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of comprehensive approach, this work reports functional characterization DnaJ chaperone related-protein (named as SPA; sugar partition-affecting) is involved tomato plants. SPA protein was found to be targeted chloroplast thylakoid membranes. SPA-RNAi plants produced more and heavier...
Summary Tocochromanols constitute the different forms of vitamin E (VTE), essential components human diet, and display a high membrane protectant activity. By combining interval mapping genome‐wide association studies (GWAS), we unveiled genetic determinants tocochromanol accumulation in tomato ( Solanum lycopersicum ) fruits. To enhance nutritional value this highly consumed vegetable, dissected natural intraspecific variability tocochromanols fruits genetically engineered their...
Phytochrome (PHY)-mediated light and temperature perception has been increasingly implicated as important regulator of fruit development, ripening, nutritional quality. Fruit ripening is also critically regulated by chromatin remodeling via DNA demethylation, though the molecular basis connecting epigenetic modifications in fruits environmental cues remains largely unknown. Here, to unravel whether PHY-dependent regulation development involves mechanisms, an integrative analysis methylome,...
Summary Phytochrome-mediated light and temperature perception has been shown to be a major regulator of fruit development. Furthermore, chromatin remodelling via DNA demethylation described as crucial mechanism behind the ripening process; however, molecular basis underlying triggering this epigenetic modification remains largely unknown. Here, through integrative analyses methylome, siRNAome transcriptome tomato fruits from phyA phyB1B2 null mutants, we report that PHYB1 PHYB2 control...
Abstract Phytochrome-mediated light and temperature perception has been shown to be a major regulator of fruit development. Furthermore, chromatin remodelling via DNA demethylation described as crucial mechanism behind the ripening process; however, molecular basis underlying triggering this epigenetic modification remains largely unknown. Here, through integrative analyses methylome, siRNAome transcriptome tomato fruits from phyA phyB1B2 null mutants, we report that PHYB1 PHYB2 control...