A5034729834- Circular RNAs in diseases
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Genetic Syndromes and Imprinting
- Cancer, Hypoxia, and Metabolism
- Metastasis and carcinoma case studies
- Kruppel-like factors research
- Gastric Cancer Management and Outcomes
- Cancer-related gene regulation
- Pancreatic and Hepatic Oncology Research
- Helicobacter pylori-related gastroenterology studies
- Signaling Pathways in Disease
- Histone Deacetylase Inhibitors Research
- Developmental Biology and Gene Regulation
- Macrophage Migration Inhibitory Factor
- Gastrointestinal Tumor Research and Treatment
- Ferroptosis and cancer prognosis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Mycobacterium research and diagnosis
- Wnt/β-catenin signaling in development and cancer
- Cardiac Structural Anomalies and Repair
Fudan University Shanghai Cancer Center
2021-2025
Shanghai Medical College of Fudan University
2021-2025
Shanghai Cancer Institute
2025
Fudan University
2024
Shanghai First People's Hospital
2018-2021
Shanghai Jiao Tong University
2018-2021
Songjiang District Central Hospital
2019-2020
Abstract Background As a novel type of noncoding RNAs, covalently closed circular RNAs (circRNAs) are ubiquitously expressed in eukaryotes. Emerging studies have related dysregulation circRNAs to tumorigenesis. However, the biogenesis, regulation, function and mechanism gastric cancer (GC) remain largely unclear. Methods The expression profile 6 pairs GC tissues adjacent non-tumor was analyzed by RNA-sequencing. Quantitative real-time PCR used determine level circCCDC9 cell lines. Then,...
Mounting evidences indicate that circular RNAs (circRNAs) play vital roles in the development and progression of various cancers. However, detail functions underlying mechanisms circRNAs gastric cancer remain largely unknown.The expression profile metastasis-related was screened by RNA-seq analysis. qRT-PCR used to determine level prognostic values circNHSL1 tissues. In vitro cell wound healing transwell (migration invasion) vivo tumorigenesis metastasis assays were performed evaluate...
Ferroptosis is a form of regulated cell death initiated by oxidative perturbations that can be blocked iron chelators and lipophilic antioxidants, ferroptosis may the silver bullet treatment for multiple cancers, including immunotherapy- chemotherapy-insensitive cancers such as pancreatic ductal adenocarcinoma (PDAC). Numerous studies have noted long non-coding RNAs (lncRNAs) regulate biological behaviour cancer cells binding to DNA, RNA protein. However, few reported role lncRNAs in...
Extensive studies have demonstrated the pivotal roles of circular RNAs (circRNAs) in occurrence and development different human cancers. However, expression regulatory circRNAs pancreatic ductal adenocarcinoma (PDAC) are unclear.CircEYA3 was explored based on Gene Expression Omnibus (GEO) dataset analysis. qRT-PCR applied to determine circRNAs, miRNAs mRNAs PDAC cells tissues. The biological circEYA3 vitro vivo were determined by performing a series functional experiments. Further, dual...
Background: Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal diseases. Although several chemotherapy regimens have been developed over past decades, few targeted therapies shown a significant improvement in overall survival, partly due to identification PDAC as single disease. Methods: Combining metabolomic analysis and immunohistochemistry staining with Oil Red O staining, for oxygen consumption rate extracellular acidification rate, we stratified pancreatic cancer cells...
Rationale: As a component of GINS complex, GINS4 is essential for initiating DNA replication and elongation the cell cycle G1/S phase in eukaryotes plays vital role normal physiological processes.However, precise functions regulation mechanisms human tumors remain elusive.Methods: expression was analyzed gastric cancer tissues by qRT-PCR western blotting, its clinical relevance studied using TMA.The biological were detected vitro vivo.cDNA array, co-IP, GST pull-down GTPase activation assays...
Podocalyxin-like 1 (PODXL) was reported to be closely associated with the development of various cancers, yet its functional roles and molecular mechanisms remain vague. The aim our study investigate clinical significance, biological function mechanism PODXL in gastric cancer (GC).The level GC tissues detected applying microarray, fresh public database (Oncomine). invasion, metastasis tumorigenesis role were performed vitro vivo. correlations between KLF4 determined microarray tissues,...
GINS complex subunit 4 (GINS4) is essential for DNA replication initiation and elongation in the G1 /S phase cell cycle eukaryotes, however, its functional roles molecular mechanisms remain unclear many aspects. Our study was designed to investigate clinical significance, biological function, mechanism of GINS4 colorectal cancer (CRC). First, we confirmed that expression significantly overexpressed CRC cells tissues. The immunohistochemical results tissue microarray from 106 patients showed...
HOXA13 is a transcription factor of the Homeobox (HOX) gene family, which highly evolutionarily conserved. upregulated and associated with oncogenic properties in some cancers. Here, we studied potential mechanism HOXA13-mediated proliferation metastasis gastric cancer (GC).Quantitative real-time PCR, Western blot, immunohistochemistry were used to detect expression levels GC. In vitro vivo assays performed investigate function GC cell proliferation, migration, invasion. RNA-Seq...
Gastric cancer (GC) is one of the digestive tract malignancies with high invasion and mortality rates. Recent studies have reported that non-coding RNAs (ncRNAs) seem to play a crucial part in many tumors. Due their stability, ncRNAs may used as novel biomarkers predict occurrence prognosis GC. Here, we measured miRNA, lncRNA cirRNA expression profiles GC patients by using microarray RNA-sequencing data from tissue samples. The diagnosis prediction model based on ncRNA signatures clinical...
Pancreatic cancer is one of the most lethal malignancies. Desmoplastic stroma and metabolic reprogramming are two hallmarks pancreatic that support its malignant biological behaviors. However, underlying mechanism by which maintain redox balance remains unclear in ductal adenocarcinoma (PDAC). Here, we demonstrated physical properties could regulate expression PIN1 cells. Moreover, found hard matrix-cultured cells induced upregulation expression. Since maintained via synergistic activation...
Abstract Background Colorectal cancer (CRC) is one of the most frequent malignancy and a leading cause cancer-related deaths. Therefore, further researches are required to identify novel more effective diagnoses molecular targets in treatment CRC. Methods C2CD4A expression CRC tissues cell lines was detected by qRT-PCR western blot. The biological functions were performed both vitro vivo . Western blot, cDNA array, IP-MS, Co-immunoprecipitation assay, Ubiquitination assay used analyze...
Background: Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal diseases. Although several chemotherapy regimens have been developed in past decades, few targeted therapy has demonstrated a significant benefit regarding overall survival, partly due to identification PDAC as single disease. Methods: Combining metabolomic analysis and immunohistochemistry staining, with oil Red O for oxygen consumption rate extracellular acidification rate, we stratified pancreatic cancer cells...
Abstract Background: Colorectal cancer (CRC) ranks as the third most frequently diagnosed and is a leading cause of cancer-related deaths. Therefore, further researches were required to identify novel more effective diagnoses molecular targets in treatment CRC. Methods: CRC fresh frozen tissues cell lines used detect C2CD4A expression by qRT-PCR western blotting. The biological functions performed vitro vivo . Western blotting, cDNA array, IP-MS, Co-IP, Ubiquitination assay analyze...