Zeni, O., Schiavoni, A. S., Sannino, A., Antolini, Forigo, D., Bersani, F. and Scarfì, M. R. Lack of Genotoxic Effects (Micronucleus Induction) in Human Lymphocytes Exposed In Vitro to 900 MHz Electromagnetic Fields. Radiat. Res. 160, 152–158 (2003).In the present study, we investigated induction genotoxic effects human peripheral blood lymphocytes after exposure electromagnetic fields used mobile communication systems (frequency MHz). For this purpose, incidence micronuclei was evaluated by...

Scarfì, M. R., Fresegna, A. M., Villani, P., Pinto, Marino, C., Sarti, Altavista, Sannino, and Lovisolo, G. Exposure to Radiofrequency Radiation (900 MHz, GSM signal) does not Affect Micronucleus Frequency Cell Proliferation in Human Peripheral Blood Lymphocytes: An Interlaboratory Study. Radiat. Res. 165, 655–663 (2006).The objective of this study was investigate whether 24 h exposure radiofrequency electromagnetic fields similar those emitted by mobile phones induces genotoxic effects...

The incidence of micronuclei was evaluated to assess the induction an adaptive response non-ionizing radiofrequency (RF) radiation in peripheral blood lymphocytes collected from five different human volunteers. After stimulation with phytohemagglutinin for 24 h, cells were exposed dose 900 MHz RF used mobile communications (at a peak specific absorption rate 10 W/kg) 20 h and then challenged single genotoxic mitomycin C (100 ng/ml) at 48 h. Lymphocytes 72 examine frequency...

The aim of this preliminary investigation was to assess whether human peripheral blood lymphocytes which have been pre-exposed non-ionizing radiofrequency fields exhibit an adaptive response (AR) by resisting the induction genetic damage from subsequent exposure ionizing radiation. Peripheral four healthy donors were stimulated with phytohemagglutinin for 24 h and then exposed 20 1950 MHz (RF, dose, AD) at average specific absorption rate 0.3 W/kg. At 48 h, cells subjected a challenge dose...

The impact of pulse repetition rate (PRR) in modulating electroporation (EP) induced by nanosecond pulsed electric fields (nsPEFs) mammalian cells was approached here performing both biological and numerical analysis. Plasma membrane permeabilization viability Jurkat were analyzed after exposure to 500, 1.3 MV/m, 40 ns PEFs with variable PRR (2-30 Hz). A finite-element model used investigate EP dynamics a single cell under the same pulsing conditions, looking at time course transmembrane...

Reversible electroporation is used to increase the uptake of chemotherapeutic drugs in local tumor treatment (electrochemotherapy) by applying pulsing protocol (8 rectangular pulses, 1000 V/cm, 100 µs) standardized framework European Standard Operating Procedure on Electrochemotherapy multicenter trial. Currently, new electrochemotherapy strategies are under development extend its applicability tumors with different histology. Electrical parameters and drug type critical factors. A possible...

Emerging technologies are considering the possible use of Terahertz radiation in different fields ranging from telecommunications to biology and biomedicine. The study potential effects on biological systems is therefore an important issue order safely develop a variety applications. This paper describes pilot devoted determine if could induce genotoxic human peripheral blood leukocytes. For this purpose, whole samples healthy donors were exposed for 20 min radiation. Since, our knowledge,...

To investigate the influence of cell cycle on adaptive response (AR) induced by exposure human blood lymphocytes to radiofrequency fields (RF).Human peripheral in G(0)-, G(1)- or S-phase were exposed for 20 hours an dose (AD) 900 MHz RF at average specific absorption rate 1.25 W/kg and then treated with a challenge (CD) 100 ng/ml mitomycin C (MMC). Un-exposed sham-exposed controls as well cells MMC alone included study. The incidence micronuclei (MN) was evaluated determine induction AR.The...

Abstract In this study, rat pheochromocytoma (PC12) cells were exposed, as a model of neuron‐like cells, to 1950 MHz radiofrequency (RF) radiation with signal used by the 3G wireless technology Universal Mobile Telecommunications System (UMTS) assess possible adverse effects. RF exposure for 24 h at specific absorption rate (SAR) 10 W/kg was carried out in waveguide system under accurately controlled environmental and dosimetric parameters. DNA integrity, cell viability, apoptosis...

Abstract The last decades have seen increased interest toward possible adverse effects arising from exposure to intense static magnetic fields. This concern is mainly due the wider and applications of such fields in industry clinical practice; among them, Magnetic Resonance Imaging (MRI) facilities are main sources for both general public (patients) workers. In recent investigations, exposures been demonstrated elicit, different cell models, permanent transient modifications cellular...

This study aims to assess whether a 1950 MHz radiofrequency (RF) electromagnetic field could protect human neuroblastoma SH-SY5Y cells against subsequent treatment with menadione, chemical agent inducing DNA damage via reactive oxygen species formation. Cells were pre-exposed for 20 h specific absorption rate of either 0.3 or 1.25 W/kg, and 3 after the end exposure, they treated 10 µM menadione (MD) 1 h. No differences observed between sham- RF-exposed samples. A statistically significant...

Scarfí, M. R., Sannino, A., Perrotta, Sarti, M., Mesirca, P. and Bersani, F. Evaluation of Genotoxic Effects in Human Fibroblasts after Intermittent Exposure to 50 Hz Electromagnetic Fields: A Confirmatory Study. Radiat. Res. 164, 270–276 (2005).The aim this investigation was confirm the main results reported recent studies on induction genotoxic effects human fibroblasts exposed intermittent (5 min field on/10 off) sinusoidal electromagnetic fields. For purpose, DNA single-strand breaks...

In this study, the induction of apoptosis after exposure to 900 MHz radiofrequency radiation (GSM signal) was investigated by assessing caspase 3 activation in exponentially growing Jurkat cells and quiescent proliferating human peripheral blood lymphocytes (PBLs). The carried out at an average specific absorption rate 1.35 W/kg a dual wire patch cell system where temperature cultures accurately controlled. After 1 h field, slight but statistically significant increase activity, measured 6...

In this study, the effect of radiofrequency (RF) exposure to 1950 MHz, Universal Mobile Telecommunication System signal, was investigated in Chinese hamster lung fibroblast cell line (V79). Genotoxic and cytotoxic effects 20‐h at specific absorption rate (SAR) values from 0.15 W/kg 1.25 were measured by means cytokinesis‐block micronucleus (MN) assay. Exposure carried out blinded under strictly controlled conditions dosimetry temperature. The RF alone four SAR tested, that is, 0.15, 0.3,...

Sannino, A., Di Costanzo, G., Brescia, F., Sarti, M., Zeni, O., Juutilainen, J. and Scarfì, M. R. Human Fibroblasts 900 MHz Radiofrequency Radiation: Evaluation of DNA Damage after Exposure Co-exposure to 3-Chloro-4-(dichloromethyl)-5-Hydroxy-2(5h)-furanone (MX). Radiat. Res. 171, 743–751 (2009).The aim this study was investigate damage in human dermal fibroblasts from a healthy subject affected by Turner's syndrome that were exposed for 24 h radiofrequency (RF) radiation at MHz. The...

Abstract This study was designed to assess if radiofrequency (RF) radiation induces oxidative stress in cultured mammalian cells when given alone or combination with ferrous ions (FeSO 4 ). For this purpose the production of reactive oxygen species (ROS) measured by flow cytometry human lymphoblastoid exposed 1950 MHz signal used third generation wireless technology Universal Mobile Telecommunication System (UMTS) at Specific Absorption Rate 0.5 and 2.0 W/kg. Short (5–60 min) long (24 h)...

A numerical model, usually employed to analyze the electroporation phenomenon in eukaryotic cells exposed high voltage electric pulses with duration ms μs time scale, is extended investigate onset and dynamics induced by nanosecond pulsed fields (nsPEFs). The model allows track primary events of process on a scale where available experimental methods do not provide reliable information. In particular solved domain, provides spatial temporal distribution transmembrane pore density fixed radii...

In the last years, radiofrequency (RF) has demonstrated that it can reduce DNA damage induced by a subsequent treatment with chemical or physical agents in different cell types, resembling adaptive response, phenomenon well documented radiobiology. Such an effect also been reported other authors both vitro and vivo, plausible hypotheses have formulated, spanning from perturbation of redox status, to repair mechanisms, stress response machinery, as possible cellular mechanisms activated RF...

Intense nanosecond pulsed electric fields (nsPEFs) interact with cellular membranes and intracellular structures. Investigating how cells respond to pulses is essential for a) development of biomedical applications nsPEFs, including cancer therapy, b) better understanding the mechanisms underlying such bioelectrical effects. In this work, we explored relatively mild exposure conditions provide insight into weak, reversible effects, laying a foundation interaction kinetics nsPEF bio-effects....

Human peripheral blood leukocytes from six volunteers were exposed to a Universal Mobile Telecommunication System (UMTS) signal (frequency carrier of 1950 MHz) for 24 h. The exposures carried out in waveguide system at specific absorption rates (SAR) 0.5 and 2.0 W/kg, each donor, sham-exposed samples also set up. alkaline comet assay was used quantify DNA damage, while cytotoxicity determined by the Trypan blue exclusion method. results obtained indicate absence genotoxic cytotoxic effects...

This study was designed to investigate the cytotoxicity of multiwalled carbon nanotube buckypaper (BP) in stimulated human peripheral blood lymphocytes. Materials & methods results: BP treatment led a delay cell growth, as proven by minor increase number over time relative that seen untreated cells, assessed trypan blue, resazurin and neutral red assays. The analysis cell-cycle profile, propidium iodide staining, indicated blocked progression arresting cells at G0/G1 phase. Moreover,...