A5024787308- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- Pancreatic and Hepatic Oncology Research
- Lung Cancer Research Studies
- Genomic variations and chromosomal abnormalities
- Epigenetics and DNA Methylation
University of Essex
2021-2025
Abstract Aging induces systematic changes in the distribution of nucleosomes, which affect gene expression programs. Here we reconstructed nucleosome maps based on cell‐free DNA (cfDNA) extracted from blood plasma using four cohorts people different ages. We show that nucleosomes tend to be separated by larger genomic distances older people, and age correlates with repeat length (NRL). Furthermore, developed first aging clock cfDNA nucleosomics. Machine learning distance distributions...
Abstract Background Nucleosome repositioning in cancer is believed to cause many changes genome organisation and gene expression. Understanding these important elucidate fundamental aspects of cancer. It also for medical diagnostics based on cell-free DNA (cfDNA), which originates from genomic regions protected digestion by nucleosomes. Results We have generated high-resolution nucleosome maps paired tumour normal tissues the same breast patients using MNase-assisted histone H3 ChIP-seq...
Abstract Nucleosome positioning is involved in many gene regulatory processes happening the cell, and it may change as cells differentiate or respond to changing microenvironment a healthy diseased organism. One important implication of nucleosome clinical epigenetics its use “nucleosomics” analysis cell-free DNA (cfDNA) for purpose patient diagnostics liquid biopsies. The rationale this that apoptotic nucleases digest chromatin dying mostly cut between nucleosomes. Thus, short pieces body...
Abstract Background Profiling circulating cell-free DNA (cfDNA) has become a fundamental practice in cancer medicine, but the effectiveness of cfDNA at elucidating tumor-derived molecular features not been systematically compared to standard single-lesion tumor biopsies prospective cohorts patients. The use plasma instead tissue guide therapy is particularly attractive for patients with small cell lung (SCLC), due aggressive clinical course this cancer, which makes obtaining exceedingly...
Abstract Nucleosome repositioning in cancer is believed to cause many changes genome organisation and gene expression. Understanding these important elucidate fundamental aspects of cancer. It also for medical diagnostics based on cell-free DNA (cfDNA), which originates from genomic regions protected digestion by nucleosomes. Here we have generated high resolution nucleosome maps paired tumour normal tissues the same breast patients using MNase-assisted histone H3 ChIP-seq compared them with...
Abstract Background Profiling circulating cell-free DNA (cfDNA) has become a fundamental practice in cancer medicine, but the effectiveness of cfDNA at elucidating tumor-derived molecular features not been systematically compared to standard single-lesion tumor biopsies prospective cohorts patients. The use plasma instead tissue guide therapy is particularly attractive for patients with small cell lung (SCLC), whose aggressive clinical course making it exceedingly challenging obtain...
Abstract Nucleosome positioning is involved in many gene regulatory processes happening the cell and it may change as cells differentiate or respond to changing microenvironment a healthy diseased organism. One important implication of nucleosome clinical epigenetics its use “nucleosomics” analysis cell-free DNA (cfDNA) for purpose patient diagnostics liquid biopsies. The rationale this that apoptotic nucleases digest chromatin dying mostly cut between nucleosomes. Thus, short pieces body...