A5091423910- Metabolism and Genetic Disorders
- Helicobacter pylori-related gastroenterology studies
- Biochemical Acid Research Studies
- Alcoholism and Thiamine Deficiency
- Peroxisome Proliferator-Activated Receptors
- Galectins and Cancer Biology
- Biochemical and Molecular Research
- Mitochondrial Function and Pathology
- Bacterial Infections and Vaccines
- Eosinophilic Esophagitis
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Amino Acid Enzymes and Metabolism
- Pesticide Exposure and Toxicity
- Pneumonia and Respiratory Infections
- Molecular Biology Techniques and Applications
- Cytomegalovirus and herpesvirus research
- RNA and protein synthesis mechanisms
- Advanced Proteomics Techniques and Applications
- Pesticide and Herbicide Environmental Studies
- Cancer-related molecular mechanisms research
- Diet and metabolism studies
- Fermentation and Sensory Analysis
- DNA and Biological Computing
- Microbial Metabolites in Food Biotechnology
- HIV/AIDS drug development and treatment
University of Siena
2016-2025
University of Trieste
2024
Siena College
2016
Center for Neurosciences
2016
National Research Council
2008
UNSW Sydney
2008
Istituto per il Sistema Produzione Animale in Ambiente Mediterraneo
2008
Siena Biotech (Italy)
2007
Istituto di Genetica Molecolare
2007
Abstract The aim of the present study was to demonstrate mitogenic and differentiating properties platelet‐rich plasma releasates (PRPr) on human chondrocytes in mono‐ three‐dimensional cultures. In order assess if PRPr supplementation could maintain chondrocyte phenotype or at least inhibit cell de‐differentiation even after several days culture, we performed a proteomic cultures independently grown, for different periods time, culture medium with FCS, serum (HS), obtained from PRP...
New pyrazolo[3,4-d]pyrimidines were synthesized and found to inhibit Src phosphorylation in a cell-free assay. Some of them significantly reduced the growth human osteogenic sarcoma (SaOS-2) cells. The best compound, terms inhibitory properties toward both SaOS-2 cells, was further investigated reduce bone resorption when used treat mouse osteoclasts, without interfering with normal osteoblast growth. Moreover, its metabolic stability prompted study on xenograft tumor model nude mice, where...
Abstract The possible role of Helicobacter pylori as a trigger for some extragastric diseases has been largely investigated in the last year. There are, fact, several studies concerning cardiovascular diseases, neurological disorders, diabetes mellitus, ear and eyes immunological hematological liver bile tract gynecological respiratory pathologies. Among them, idiopathic sideropenic anemia thrombocytopenic purpura still remain showing most convincing results. Concerning ischemic heart...
Alkaptonuria (AKU) is an ultra-rare disease developed from the lack of homogentisic acid oxidase activity, causing (HGA) accumulation that produces a HGA-melanin ochronotic pigment, unknown composition. There no therapy for AKU. Our aim was to verify if AKU implied secondary amyloidosis. Congo Red, Thioflavin-T staining and TEM were performed assess amyloid presence in specimens (cartilage, synovia, periumbelical fat, salivary gland) HGA-treated human chondrocytes cartilage. SAA SAP...
Abstract Heat treatment of milk induces the Maillard reaction between lactose and proteins; in this context, β‐lactoglobulin α‐lactalbumin adducts have been used as markers to monitor quality. Since some proteins reported essential for delivery microelements and, being resistant against proteolysis gastrointestinal tract, also contributing acquired immune response pathogens stimulation cellular proliferation, it is crucial systematically determine subproteome affected by a careful evaluation...
// Federico Galvagni 1, * , Federica Nardi Marco Maida 1 Giulia Bernardini Silvia Vannuccini 2 Felice Petraglia Annalisa Santucci Maurizio Orlandini Department of Biotechnology, Chemistry and Pharmacy, University Siena, 2-53100 Italy Molecular Developmental Medicine, Obstetrics Gynecology, 53100 These authors contributed equally to this work Correspondence to: Orlandini, e-mail: maurizio.orlandini@unisi.it Keywords: angiogenesis, signal transduction, C1qRp, Src, Cbl Received: June 29, 2015...
Background . Alkaptonuria, a rare autosomal recessive metabolic disorder caused by deficiency in homogentisate 1,2-dioxygenase activity, leads to accumulation of oxidised homogentisic acid cartilage and collagenous structures present all organs tissues, especially joints heart, causing pigmentation called ochronosis. A secondary amyloidosis is associated with AKU. Here we report study an aortic valve from AKU patient. Results Congo Red birefringence, Th-T fluorescence, biochemical assays...
Osteosarcoma is the most frequent primitive malignant tumor of skeletal system, characterized by an extremely aggressive clinical course that still lacks effective treatment. Src kinase seems to be involved in osteosarcoma phenotype. We show treatment human cell lines with a new pyrazolo[3,4-d]pyrimidine derivative inhibitor, namely SI-83, impaired viability, half-maximal inhibitory concentration 12 microM nonstarved cells and kinetic different from known for inhibitor PP2. Analysis terminal...
Alkaptonuria (AKU) is a rare genetic disease associated with the accumulation of homogentisic acid (HGA) and its oxidized/polymerized products in connective tissues up to deposition melanin-like pigments (ochronosis). Since little known on effects HGA metabolites articular cells, we carried out proteomic redox-proteomic analysis investigate how ascorbic (ASC) affect human chondrocytic protein repertoire. We settled an vitro model using cell line evaluate 0.33 mM HGA, alone or combined ASC....
Abstract Alkaptonuria (AKU) is a rare genetic disease associated with the accumulation of homogentisic acid (HGA) and its oxidized/polymerized products which leads to deposition melanin‐like pigments (ochronosis) in connective tissues. Although numerous case reports have described ochronosis joints, little known on molecular mechanisms leading such phenomenon. For this reason, we characterized biochemically chondrocytes isolated from ochronotic cartilage AKU patients. Based macroscopic...
Abstract Alkaptonuria (AKU) results from defective homogentisate1,2‐dioxygenase (HGD), causing degenerative arthropathy. The deposition of ochronotic pigment in joints is so far attributed to homogentisic acid produced by the liver, circulating blood and accumulating locally. Human normal AKU osteoarticular cells were tested for HGD gene expression RT‐PCR, mono‐ 2D‐Western blotting. was revealed chondrocytes, synoviocytes, osteoblasts. Furthermore, confirmed Western blotting, that also...
Objective. Alkaptonuria (AKU) is an ultra-rare autosomal recessive disease that currently lacks appropriate therapy. Recently we provided experimental evidence AKU a secondary serum amyloid A (SAA)-based amyloidosis. The aim of the present work was to evaluate use antioxidants inhibit SAA and pro-inflammatory cytokine release in AKU. Methods. We adopted human chondrocytic cell model anti-amyloid capacity set had previously been shown counteract ochronosis model. Amyloid presence evaluated by...
To verify a possible association between overall H. pylori and CagA+ infection autoimmune thyroid diseases (AITDs).Consecutive patients with AITDs admitted to one single centre of Endocrinology during solar year were examined. The diagnoses Hashimoto thyroiditis (HT) in 76, Graves' Disease (GD) 39, aspecific (AT) 44 patients. Controls 136 individuals without AITDs. Median values fT3, fT4, anti-thyreoglobulin (Tg) antibodies, IL-1β, IL-6, TNF-α compared those controls. CagA status determined...
Alkaptonuria (AKU) is an ultra-rare genetic disorder caused by mutations in the homogentisate 1,2-dioxygenase (HGD) gene, leading to accumulation of homogentisic acid (HGA). Current treatment options are limited, with Nitisinone (Orfadin or NTBC) being only approved drug. However, its long-term use raises concerns due significant adverse effects, highlighting urgent need for safer alternatives. AKU manifests progressive and often painful symptoms, severely impacting patients’ quality life....
Changes in expression profiles for 17 proteins were ascertained human mature osteoblasts compared to pre-osteoblasts (differentiation markers). A differential approach was used highlight proteomic changes between osteosarcoma cells and osteoblasts, showing a relative over-expression of 8 (proliferation tumor indicators), as well under-expression also found down-regulated (specific markers osteoblast differentiation). Our findings confirmed the differences cell lines primary cultures...