A5021688468- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Oral and Maxillofacial Pathology
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Head and Neck Cancer Studies
- Cancer, Hypoxia, and Metabolism
- Oral Health Pathology and Treatment
- Periodontal Regeneration and Treatments
- MicroRNA in disease regulation
- Bone Tissue Engineering Materials
- Planarian Biology and Electrostimulation
- Cancer Immunotherapy and Biomarkers
- Angiogenesis and VEGF in Cancer
- thermodynamics and calorimetric analyses
- Berberine and alkaloids research
- Computational Drug Discovery Methods
- Artificial Intelligence in Healthcare
- Cancer and Skin Lesions
- Aldose Reductase and Taurine
- Genetic and rare skin diseases.
- Congenital heart defects research
- Phytochemicals and Medicinal Plants
- Autophagy in Disease and Therapy
Dr D Y Patil Dental College & Hospital
2018-2021
Biogen (India)
2021
Jazan University
2021
Dr. D.Y. Patil Vidyapeeth, Pune
2018-2019
Dental pulp stem cells have emerged as a preferred source of mesenchymal cells, because its easy availability and high cell content. is specific fibrous tissue that contains heterogeneous populations odontoblasts, fibroblasts, pericytes, progenitors, leukocytes neuronal cells. In this study, we propose sustained explant culture simple, economical efficient process to isolate dental from human Tissue. Historically cultures were used get fibroblast embryonic chick heart using plasma clot...
Stem cells from human exfoliated deciduous teeth (SHEDs) are considered a type of mesenchymal stem (MSCs) because their unique origin the neural crest. SHEDs can self-renewal and multi-lineage differentiation with ability to differentiate into odontoblasts, osteoblast, chondrocytes, neuronal cells, hepatocytes, adipocytes, etc. They emerging as an ideal source MSCs easy availability extraordinary cell number. Ascorbic acid, or vitamin C, has many cell-based applications, such bone...
Objective: To demonstrate the levels of parathyroid hormone secretion and genetic expressions (PTH) PTH1 receptor (PTH1R) genes in dental pulp stem cells (DPSCs) from different age groups before after induction osteogenic differentiation. In addition, we also wanted to check their correlation with degree Methods: Human primary DPSCs three (milk tooth (SHEDs), 7–12 years old; young (yDPSCs), 20–40 old (oDPSCs), 60+ old) were characterized for mesenchymal cell (MSC) markers. subjected...
To optimize and characterize anticancer drugs conjugated biodegradable scaffold material. evaluate drug release profile of the effect mediated localized chemotherapy on cancer cell lines including oral, breast prostate in terms apoptosis assay, proliferation assay gene expression analysis. analyse results for effectiveness local delivery different lines. A lyophilized hydrogel medium molecular weight chitosan, (in three concentrations viz; 0.5 μM, 1 μM 1.5 μM) sodium triphosphate is...
Background: Stem cell therapy has become an advanced and state-of-the-art procedure to regenerate lost tissues of the human body. Cartilage repair is a challenging task in which stem cells find potential application. One important biologic modifiers that can cause chondrogenic differentiation taurine. However, taurine not been investigated for its effects on dental pulp derived (DPSC) differentiation. Objective: The objective study was investigate if administration DPSCs heralds as...
Background: Growth factors and cytokines responsible for the regenerative potential of dental pulp mesenchymal stem cell secretome (DPMSC-S) are implicated in oral carcinogenesis. The impact effects these secretory on cancer cells must be understood order to ensure their safe application patients. Objective: We aimed quantify growth DPMSC-S assess effect proliferation. Materials methods: DPMSCs were isolated from patients with healthy teeth (n = 5) that indicated extraction orthodontic...
Vitamin D is a commonly used bone modulator in regenerative medicine. Several modalities have been explored for the delivery of vitamin including nanoparticles and scaffold. The present study aimed to assess potential use bio-degradable chitosan scaffold D. objectives included fabrication scaffold, integration into characterization integrated Characterization was carried out using, X-ray diffraction, Fourier transform infrared spectroscopy, differential scanning calorimetry. structure...
The action of stem cells is mediated by their paracrine secretions which comprise the secretory profile. Various approaches can be used to modify profile cells. Creating a hypoxic environment one method. present study aims demonstrate influence CoCl2 in generating conditions dental pulp cell (DPSCs) culture, and effect this on DPSCs that were isolated from human permanent teeth characterized treated with different concentrations assess viability an...
Tinospora cordifolia (Thunb.) Miers (Giloy) has been applied successfully as an anti-inflammatory, anti-diabetic, and even anti-cancer agent. Yet, to date, the application of Giloy not explored concerning oral cancer.To assess effect T extract (TcE) on cancer cell line.AW13516 (oral line) cells were treated with prepared aqueous TcE for 24 h at various concentrations ranging between 5 μg/ml 100 compared control (cells without treatment). Thee extracts apoptosis was assessed by through...
Normal and chronic wound healing is a global challenge. Electrotherapy has emerged as novel efficient technique for treating such wounds in recent decades. Hydrogel applied to the uniformly distribute electric current an important component electrotherapy. This study reports development efficacy testing of vitamin D entrapped polyaniline (PANI)-chitosan composite hydrogel
The SARS-Cov-2(severe acute respiratory syndrome coronavirus 2) infection affecting human populations worldwide is now a very concerning issue considering the morbidity and mortality rates. Despite several measures followed by medical fraternity general public, there no resolution. Therapeutic to tackle have been based on researching new designer drug molecules that could prevent viral entry into host. Melatonin has tried as an adjuvant in management of COVID 19(coronavirus disease) illness...
Gingival mesenchymal stem cells (GMSCs) have significant regenerative potential. Their potential applications range from the treatment of inflammatory diseases, wound healing, and oral disorders. Preconditioning these can optimize their biological properties. Hypoxia preconditioning MSCs improves cell properties like proliferation, survival, differentiation This research explored possible impact hypoxia on pluripotent that GMSCs possess. We evaluated morphology, stemness, neurotrophic...
Background: dental pulp-derived stem cells are easy to access and collect an excellent source of for regenerative therapy. These can interact with many biomolecules scaffolds pass on the instructive signals sites regeneration where they used. In this regard cordycepin, a potential biomolecule derived from medicinal mushrooms spectrum bioactive properties such as antioxidant, anti-inflammatory, anticancer has not yet been tested its effect human pulp cells. Objective: objective present study...
Periodontitis is a condition that occurs because of inflammation-mediated tissue degeneration. Many studies have been conducted to identify inflammatory molecules in periodontitis, but the well-defined role cells from immune system progression periodontitis as well gingival degeneration has not appropriately established. The objective present study was characterize monocytes isolated crevicular fluid (GCF) patients with periodontitis. GCF obtained and healthy controls. Cytokine levels CCL2...
Autogenous gingival grafts used for root coverage or augmentation procedures often result in donor site morbidity. Living cellular constructs as an exogenous alternative have been proven to be associated with lower With the available background information, present study aims assess if quercetin-induced living cell constructs, derived from dental pulp stem cells, potential applied a tool soft tissue augmentation. The characterized cells (positive CD73, CD90, and negative CD34, HLA-DR) were...
We evaluated the role of allicin in periodontitis using an silico and vitro design. An docking analysis was performed to assess plausible interactions between PD-L1. The cytokine profile gingival crevicular fluid (GCF) samples obtained from patients estimated by cytometric bead array. CD3+ lymphocytes isolated peripheral blood were sorted characterized immunomagnetic techniques. Cultured expanded treated with GCF induce T-cell exhaustion. Optimum concentrations added exhausted compare...
Objective: To examine the effect of Cordycepin on viability, proliferation, and migratory properties dental pulp-derived mesenchymal stem cells. Materials methods: The pulp was derived from human premolar teeth extracted for orthodontic purposes after obtaining informed consent. samples were transferred to laboratory processing. DPSCs expanded characterized using flow cytometry differentiation bone, adipose, cartilage cells examined. MTT Assay performed various concentrations Cordycepin....
Stem cell therapy is an evolving treatment strategy in regenerative medicine. Recent studies report stem cells from human exfoliated deciduous teeth could complement the traditional mesenchymal sources. exhibit characteristics with multilineage differentiation potential. Mesenchymal are widely investigated for and disease modeling. Although many research being conducted to address challenges of clinics, most still infancy. Host microenvironment one major factors affecting homing transplanted...
Image processing has become a vital part of real time applications in today's world. However, image on mobile robots, drones and/or miniature robotic cars is still complex task due to its high computational needs and communication overheads. The computation power currently used embedded boards like Raspberry Pi, BeagleBone Black sufficient but not enough for efficient applications. This paper represents the combination two disciplines related processing: Object recognition...