A5095092072- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Plant Reproductive Biology
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Plant Pathogens and Fungal Diseases
- Plant Physiology and Cultivation Studies
Institute for Systems Biology
2024
InSysBio (Russia)
2024
At present, bacteriophages are considered as an alternative to antibiotics in prevention and treatment of bacterial infections, particular cholera. The aim the work was demonstrate a method obtain synthetic bacteriophage against Vibrio cholerae . Vibriophage N4 selected subject for study. Materials methods genome sequence vibriophage (38.5 kb) taken from NCBI GenBank database. divided into gene blocks 1500–2000 bp. blocks, turn, were split oligonucleotides. Sequence partitioning carried out...
The standardization of DNA fragment assembly methods for many tasks synthetic biology is crucial. This necessary synthesizing a wider repertoire sequences, as well further automation and miniaturization such reactions. In this work, we proposed conditions the fragments from chemically synthesized oligonucleotides identified errors occurring in sequence under these conditions. Additionally, combining into larger fragments. We showed that optimized are suitable wide range sequences.
Introduction WhiA is a conserved protein found in numerous bacteria. It consists of an HTH DNA-binding domain linked with homing endonuclease (HEN) domain. one the most transcription factors reduced bacteria class Mollicutes. Its function Mollicutes unknown, while it well-characterized Streptomyces . Here, we focused on from Mycoplasma gallisepticum Methods We used combination molecular dynamics, EMSA, MST and AFM to study ATP-binding properties M. The transcriptional repressor was...
WhiA is a conserved protein that widespread in several groups of bacteria. Its function significantly differs across bacterial taxons and poorly understood except for its activity sporulation control Streptomyces. We demonstrated mycoplasmas (Mollicutes) transcriptional regulator the ribosomal proteins operon an ATP sensor. Knockdown whiA gene results significant growth retardation which indicates importance.