A5060138806- Advanced Fluorescence Microscopy Techniques
- Invertebrate Immune Response Mechanisms
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Neurobiology and Insect Physiology Research
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Insect Utilization and Effects
- Advanced Biosensing Techniques and Applications
- Insect symbiosis and bacterial influences
- Click Chemistry and Applications
- Mosquito-borne diseases and control
- Bacteriophages and microbial interactions
- Genomics and Chromatin Dynamics
- bioluminescence and chemiluminescence research
- Immunotherapy and Immune Responses
Wellcome/MRC Cambridge Stem Cell Institute
2020-2023
Stem Cell Institute
2023
European Molecular Biology Laboratory
2023
Heidelberg University
2023
University of Cambridge
2023
Institute for Stem Cell Biology and Regenerative Medicine
2020
Vellore Institute of Technology University
2019
Abstract To understand how the nucleosome remodeling and deacetylase (NuRD) complex regulates enhancers enhancer–promoter interactions, we have developed an approach to segment extract key biophysical parameters from live-cell three-dimensional single-molecule trajectories. Unexpectedly, this has revealed that NuRD binds chromatin for minutes, decompacts structure increases enhancer dynamics. We also uncovered a rare fast-diffusing state of found restricts time spent in state. Hi-C...
Studies in different animal model systems have revealed the impact of odors on immune cells; however, any understanding why and how control cellular immunity remained unclear. We find that Drosophila employ an olfactory-immune cross-talk to tune a specific cell type, lamellocytes, from hematopoietic-progenitor cells. show neuronally released GABA derived upon olfactory stimulation is utilized by blood-progenitor cells as metabolite through its catabolism, these stabilize Sima/HIFα protein....
Abstract Enhancer-promoter dynamics are crucial for the spatiotemporal control of gene expression, but it remains unclear whether these controlled by chromatin regulators, such as nucleosome remodelling and deacetylase (NuRD) complex. The NuRD complex binds to all active enhancers modulate transcription here we use Hi-C experiments show that blurs TAD boundaries increases proximity intermediate-range (~1 Mb) genomic sequences enhancer-promoter interactions. To understand alters 3D genome...
Ribosome assembly is one of the most fundamental processes in gene expression and has served as a playground to investigate molecular mechanisms how protein-RNA complexes (RNPs) assemble. The bacterial ribosome composed around 50 ribosomal proteins several which are co-transcriptionally assembled on ~4,500 nucleotides long pre-rRNA transcript that further processed modified during transcription, entire process taking 2 minutes vivo assisted by dozens factors. How this complex works so...
SUMMARY Drosophila blood-progenitor cells generate an inflammatory cell-type termed lamellocyte, in response to parasitic wasp-infections. In this study we show that olfaction primes lamellocyte potential. Specifically, larval odor-detection mediated release of systemic γ-aminobutyric acid (GABA) from neurosecretory cells, is detected and internalized by blood progenitor-cells. GABA catabolism through the GABA-shunt pathway prevents Sima (HIFα) protein degradation. necessary sufficient for...
Drosophila blood-progenitor cells generate an inflammatory cell-type termed lamellocyte, in response to parasitic wasp-infections. In this study we show that olfaction primes lamellocyte potential. Specifically, larval odor-detection mediated release of systemic γ-aminobutyric acid (GABA) from neurosecretory cells, is detected and internalized by blood progenitor-cells. GABA catabolism through the GABA-shunt pathway prevents Sima (HIFα) protein degradation. necessary sufficient for...