A5063734894- Heavy metals in environment
- Retinoids in leukemia and cellular processes
- Estrogen and related hormone effects
- Genomics and Chromatin Dynamics
- Groundwater flow and contamination studies
- Mine drainage and remediation techniques
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Groundwater and Isotope Geochemistry
- Arsenic contamination and mitigation
- Mercury impact and mitigation studies
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Radioactive contamination and transfer
- Environmental Toxicology and Ecotoxicology
- Agriculture and Rural Development Research
- Radioactive element chemistry and processing
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- CRISPR and Genetic Engineering
- Muscle Physiology and Disorders
- Clay minerals and soil interactions
- RNA modifications and cancer
- Radioactivity and Radon Measurements
- Nuclear Receptors and Signaling
Institut de génétique et de biologie moléculaire et cellulaire
2006-2021
Inserm
2006-2021
Université de Strasbourg
1999-2021
Centre National de la Recherche Scientifique
2010-2021
Centre de Recherche et d’Enseignement de Géosciences de l’Environnement
2005-2019
Sustainable Europe Research Institute
2012-2018
Aix-Marseille Université
2011-2015
Environnements et Paléoenvironnements Océaniques et Continentaux
2014
Collège de France
1996-2012
Institut de Recherche pour le Développement
2012
The retinoic acid (RA) signal, produced locally from vitamin A by retinaldehyde dehydrogenase (Raldh) and transduced the nuclear receptors for retinoids (RA receptor 9- cis -RA receptor), is indispensable ontogenesis homeostasis of numerous tissues. We demonstrate that Raldh3 knockout in mouse suppresses RA synthesis causes malformations restricted to ocular nasal regions, which are similar those observed A-deficient fetuses and/or retinoid mutants. notably choanal atresia (CA), responsible...
We have characterized seven murine retinoic acid receptor gamma cDNA isoforms (mRAR-gamma 1 to -gamma 7) generated by alternative splicing of at least exons. These differ from one another in their 5' untranslated region and two cases 2) N-terminal A region, which is known be important for differential transactivation other nuclear receptors. mRAR-gamma 2, the predominant isoforms, are differentially expressed adult tissues during embryogenesis. Most notably, skin contains almost exclusively...
Background Atherosclerosis affects certain but not all vascular beds of the human circulation. Its molecular mechanisms are only partially understood. Human connective tissue growth factor (hCTGF) is a novel cysteine-rich, secreted polypeptide. hCTGF implicated in formation, which may play an important role atherosclerosis. Methods and Results By using differential cloning technique, we isolated cDNA clone from aorta library, identical to hCTGF. Northern analysis shows that mRNA was...
Polarized intracellular trafficking in epithelia is critical development, immunity, and physiology to deliver morphogens, defensins, or ion pumps the appropriate membrane domain. The mechanisms that control apical remain poorly defined. Using Caenorhabditis elegans, we characterize a novel secretion pathway involving multivesicularbodies release of exosomes at plasma membrane. By means two different genetic approaches, show membrane-bound V0 sector vacuolar H+-ATPase (V-ATPase) acts this...
Using genetic approaches in the mouse, we show that primary target tissue of retinoic acid (RA) action during eye morphogenesis is not retina nor corneal ectoderm, which both express RA-synthesizing retinaldehyde dehydrogenases (RALDH1 and RALDH3), but neural crest cell-derived periocular mesenchyme (POM), devoid RALDH. In POM, effects paracrine RA signal are mediated by nuclear receptors heterodimers RXRα/RARβ RXRα/RARγ. These appear to control: (1) remodeling POM through activation...
Cinnamate 4-hydroxylase [CA4H; trans-cinnamate,NADPH:oxygen oxidoreductase (4-hydroxylating), EC 1.14.13.11] is a cytochrome P450 that catalyzes the first oxygenation step of general phenylpropanoid metabolism in higher plants. The compounds formed are essential for lignification and defense against predators pathogens. We recently reported purification this enzyme from Mn(2+)-induced Jerusalem artichoke (Helianthus tuberosus L.) tuber tissues. Highly selective polyclonal antibodies raised...
Monospecific antibodies directed against the chicken progesterone receptor (PR) form B were used to screen a randomly primed phage lambda gt11 cDNA expression library prepared from size-fractionated oviduct mRNA. Two independent immunoreactive clones, cPR1 and cPR2, isolated. Antibodies selected anti-PR antiserum on matrices of cPR2 fusion proteins detected two electrophoretic immunoblots crude purified PR preparations. These had same apparent molecular weights as did forms A crosslinked...
Abstract Cells dedicate significant energy to build proteins often organized in multiprotein assemblies with tightly regulated stoichiometries. As genes encoding subunits assembling a multisubunit complex are dispersed the genome of eukaryotes, it is unclear how these protein complexes assemble. Here, we show that mammalian nuclear transcription (TFIID, TREX-2 and SAGA) composed large number subunits, but lacking precise architectural details built co-translationally. We demonstrate...
Abstract Lysine acetylation is a widespread post-translational modification regulating various biological processes. To characterize cellular functions of the human lysine acetyltransferases KAT2A (GCN5) and KAT2B (PCAF), we determined their acetylome by shotgun proteomics. One newly identified KAT2A/2B substrate polo-like kinase 4 (PLK4), key regulator centrosome duplication. We demonstrate that acetylate PLK4 domain on residues K45 K46. Molecular dynamics modelling suggests K45/K46 impairs...
Retinoic acid (RA), the active derivative of vitamin A, is involved in various developmental and homeostatic processes. To define whether certain events are particularly sensitive to a decrease embryonic RA levels, we generated mice bearing hypomorphic allele RA-synthesizing enzyme Raldh2. The resulting mutant mice, which die perinatally, exhibit features human DiGeorge syndrome (DGS) with heart outflow tract septation defects anomalies aortic arch-derived head neck arteries,...