The aim of the study is to investigate possibilities using fatty acids erythrocyte membranes and blood serum differentiate steatosis steatohepatitis in patients with liver disease (FLD) various origins. Material methods . included 84 men FLD origins (alcoholic, non-alcoholic, mixed, i.e. alcoholic + metabolic), average age 48.4 ± 13.9 years, fibrosis degree 0-1 (FibroScan® 502 Echosens, France). Using tests NashTest, AshTest as part FibroMax, ActiTest FibroTest (BioPredictive, France), 44...

The aim of the study is to investigate association rs2306283 and rs4149056 variants SLCO1B1 gene with benign unconjugated hyperbilirubinemia. Materials methods . A case-control design was employed. group Gilbert syndrome (GS) phenotype comprised 414 individuals (mean age 36.7 ± 15.9 years, 49.8% men). control consisted 429 38.5 14.3 52.2% men) randomly selected from DNA banks MONICA project participants, young adults aged 25–44 participants in a cross-sectional schoolchildren Novosibirsk....

A biosensor based on field-effect transistors silicon-on-insulator structures (SOI-biosensor) is a high-potential device for detection of biological molecules, instance, such as troponin I; the allows conducting label-free real-time analysis.

Aim. To study changes in the electrical and viscoelastic parameters of erythrocytes using method dielectrophoresis patients with manifestations metabolic syndrome who underwent COVID-19, when exposed to drug citicoline an vitro experiment reduce severity microcirculatory disorders.
 Materials methods. 31 men were examined (50.6±9.9 years), COVID-19 convalescences, within 8 12 months after disease, diagnosis was confirmed by PCR, ELISA. The studied twice: initial levels indicators...

Aim . To evaluate the effectiveness of automated Sanger sequencing UGT1A1 gene to search for pathogenic mutations in individuals with Gilbert syndrome phenotype. Materials and methods Automated exons part promoter was carried out 24 people unconjugated hyperbilirubinemia, whom all other causes except genetic ones were excluded DNA analysis performed determine number TA repeats (rs3064744). Distribution rs3064744 genotypes group following: 5 – 7TA/7TA genotype, 6TA/6TA 12 6TA/7TA 1 person...

The aim of the study was to search for associations benign unconjugated hyperbilirubinemia phenotype with rs1799945 (H63D), rs1800562 (C282Y), rs1800730 (S65C) mutations HFE gene, rs113993960 (ΔF508) CFTR rs28929474 (PIZ), rs17580 (PIS) SERPINA1 gene. design is case-control. group Gilbert's syndrome (GS) (n=414; mean age - 36.7±15.9 years; 49.8% men) formed by gastroenterologists, and included individuals who underwent a standard clinical examination. known causes were excluded from group....

Introduction. Fatty liver disease is the largest contributor to burden of chronic worldwide. Current approaches do not allow sufficient differentiation between alcoholic and non-alcoholic etiology process. Aim. Create diagnostic panels including electrical viscoelastic parameters erythrocytes differentiate fatty genesis. Materials methods. The study included 38 men (47.5 ± 2.9 years) with NAFLD; 31 (AFLD) (45.1 3.1 according ultrasound abdominal organs, degree fibrosis did exceed F1...

The objective is to measure the level of fatty acids in erythrocyte membranes and serum patients with colorectal cancer. Materials methods. study group included 100 diagnosed cancer (57 men 43 women). control 24 reasonably healthy people (14 10 women) matched for age sex, without malignant cancers or manifested pathology internal organs. Results. Decreased levels saturated, monounsaturated increased polyunsaturated (PUFAs) (p <0.0001–0.05) were observed. omega-3 PUFAs exceeded individuals...

Hemorheological disorders in structural and functional parameters of erythrocytes are involved the pathological process type 2 diabetes mellitus (DM). Aim: to investigate feasibility differential diagnosis degrees rheological disturbances patients with DM by dielectrophoresis erythrocytes. Methods: 62 subjects (58.7 ± 1.6 years) diagnosed according criteria ADA were subdivided into two groups: medium (n = 47) high 15) risk microcirculatory (EASD, 2013). Electric viscoelastic determined using...

The SOI-FET biosensor (silicon-on-insulator field-effect transistor) for virus detection is a promising device in the fields of medicine, virology, biotechnology, and environment. However, applications modern biosensors face numerous problems require improvement. Some these can be attributed to sensor design, while others technological limitations. aim this work conduct theoretical investigation "antibody + antigen" complex (AB AG) processes biosensor, which may also solve some...

Possible early diagnostic application of optical methods (dielectrophoresis, spectral and imaging ellipsometry, Fourier-transform infrared spectroscopy, Raman spectroscopy) in studies red blood cells serum patients with diffuse liver disease varying degrees fibrosis has been evaluated. Application combined was confirmed to significantly improve the performance sensitivity, specificity, accuracy index as well achieve reliable results diagnosis both severe slight ulterior fibrosis. Identified...

Thirty-seven males with non-alcoholic staatohepatitis (NASH) (aged 39 - 62) were included in the study. Clinical, biochemical and instrumental studies used to verify diagnosis. The control group was comprised of thirty-three comparable age without confirmed signs liver diseases other pathology inner organs. Dielectrophoresis applied evaluate structural-and-functional erythrocytic parameters. patients NASH erythrocytes tended differ their much lower amplitude deformation, polarizability,...

Purpose of the work: to analyze research performed in last decade on study serum markers used diagnose inflammatory bowel diseases (IBD), differential diagnosis nosological forms IBD, predict course disease, response ongoing therapy, as well recurrence disease. Materials and methods. A search was carried out text databases “Scopus”, “Web Science”, “PubMed” using keywords from literature sources 10 years about blood biomarkers for diagnosis, evaluation therapeutic efficacy, monitoring disease...

The purpose of the study: to study possibilities using fatty acids erythrocyte membranes in differentiating patients with liver disease alcoholic, non-alcoholic and mixed genesis (metabolic + alcohol). Material methods. 38 men (average age 47.5 ± 2.9 years) (NAFLD); 31 alcoholic (AFLD) (45.1 3.1 30 (48.2 3.2 alcoholic) were examined. degree fibrosis groups did not exceed grade I. composition levels (FA) erythrocyte,s studied gas chromatography/mass spectrometry, a system based on three...

The 2020 coronavirus infection pandemic has potentiated a large number of studies in the world on etiopathogenesis, clinical and morphological manifestations COVID-2019 infection. This review presents biochemical, molecular genetic aspects COVID-2019.

4 Федеральное государственное бюджетное учреждение науки Новосибирский

The aim of the work is to study differences in changes viscoelastic parameters erythrocytes (amplitude deformation, summarized viscosity and rigidity) dynamics interaction with ethanol vitro patients non-alcoholic (NAFLD) alcoholic (AFLD) fatty liver disease for use differential diagnostics. Materials methods. 50 men were examined, 24 - NAFLD (46.5±1.5 years), 26 AFLD (48.6±1.2 years). was carried out by dielectrophoresis an electro-optical cell detection system. levels evaluated before...