A5053480631- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Genetic Neurodegenerative Diseases
- Neurogenetic and Muscular Disorders Research
- Amyotrophic Lateral Sclerosis Research
- Tendon Structure and Treatment
- Myofascial pain diagnosis and treatment
- Virus-based gene therapy research
- Nutrition and Health in Aging
- Shoulder Injury and Treatment
- Mitochondrial Function and Pathology
- Viral Infections and Immunology Research
- Bee Products Chemical Analysis
Sorbonne Université
2024-2025
Inserm
2024-2025
Institut de Myologie
2024-2025
Centre de Recherche en Myologie
2024-2025
Sorbonne University Abu Dhabi
2024
Sapienza University of Rome
2019-2024
Istituto Pasteur
2022-2023
We performed scRNA-seq/snATAC-seq of skeletal muscles post sciatic nerve transection to delineate cell type-specific patterns gene expression/chromatin accessibility at different time points post-denervation. Unlike myotrauma, denervation selectively activates glial cells and Thy1/CD90-expressing mesenchymal cells. Glial expressed Ngf receptor (Ngfr) were located near neuromuscular junctions (NMJs), close cells, which provided the main cellular source NGF Functional communication between...
Here, we report on the identification of Itga7-expressing muscle-resident glial cells activated by loss neuromuscular junction (NMJ) integrity. Gene expression analysis at bulk and single-cell level revealed that these are distinct from muscle satellite cells. We show a selective activation expansion Itga7+ occur in response to nerve lesion. Upon activation, glial-derived progenies expressed neurotrophic genes, including growth factor receptor, which enables their isolation FACS. also genes...
To assess the ultrasound features in patients with plantar fasciopathy before and after extracorporeal shock waves therapy (ESWT), using conventional grey-scale imaging both strain (SE) shear wave (SWE) elastosonographic evaluation.Consecutive of sexes attending our outpatient's clinic, diagnosis unilateral fasciopathy, were enrolled. Patients treated 3 sessions ESWT once a week, underwent clinical evaluation at baseline one three months treatment. Roles Maudsley score (RM), visual analog...
Highlights•Spatial transcriptomics reveals distinct vulnerabilities among muscle fibers in ALS•Muscle fiber defects are associated with disruptions polyamine metabolism•AMD1 inhibition leads to skeletal impairment•Restoring balance enhances phenotype ALS-affected miceSummaryAmyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease causing progressive paralysis due motor neuron degeneration no curative therapy despite extensive biomedical research. One of the primary targets...
<title>Abstract</title> Sarcoglycanopathies are heterogeneous proximo-distal diseases presenting severe muscle alterations. These caused by mutations in genes coding for one of the four sarcoglycan transmembrane proteins, which form complex (SGC). Little is known about different roles SGC beyond dystrophin glycoprotein (DGC) structural role. Here, we show that proteins enriched at post-synaptic membrane neuromuscular junctions (NMJs). Using a mouse model lacking beta-sarcoglycan subunit,...
SUMMARY Striated muscle is a highly organized structure composed by well-defined anatomical domains with integrated but distinct assignments. So far, the lack of direct correlation between tissue architecture and gene expression has limited our understanding how each unit responds to physio-pathologic contexts. Here, we show combined use spatially resolved transcriptomics immunofluorescence can bridge this gap enabling unbiased identification such characterization their response external...