A5070652282- Plant tissue culture and regeneration
- Berberine and alkaloids research
- Essential Oils and Antimicrobial Activity
- Synthesis and bioactivity of alkaloids
- Plant biochemistry and biosynthesis
- Plant-based Medicinal Research
- Phytochemistry and Biological Activities
- Botanical Research and Chemistry
- Natural product bioactivities and synthesis
- Seed Germination and Physiology
- Chemical synthesis and alkaloids
- Light effects on plants
- Transgenic Plants and Applications
- Phytochemicals and Antioxidant Activities
- Plant Genetic and Mutation Studies
- Bee Products Chemical Analysis
- Analytical Chemistry and Chromatography
- Medicinal Plants and Neuroprotection
- Plant-Microbe Interactions and Immunity
- Traditional Chinese Medicine Analysis
- Microbial Metabolites in Food Biotechnology
- Insect and Pesticide Research
- Medicinal plant effects and applications
- Insect Pest Control Strategies
- Alkaloids: synthesis and pharmacology
Wroclaw Medical University
2016-2025
Pharmaceutical Biotechnology (Czechia)
2022
Nicolaus Copernicus University
2016
University of Bydgoszcz
2016
University of Wrocław
2014-2016
University of Szczecin
2003-2014
This review article is a comprehensive and current overview on chalcones, covering their sources, identification methods, properties with particular focus applications in the agricultural sector. The widespread use of synthetic pesticides has not only led to increased resistance among weeds pests, resulting economic losses, but it also raised significant health concerns due overuse these chemicals. In line European Green Deal 2030 its Farm Fork strategy, there targeted 50% reduction chemical...
Chelidonium majus (Papaveraceae) extracts exhibit antimicrobial activity due to the complex alkaloid composition. The aim of research was evaluate potential from wild plants and in vitro cultures, as well seven major individual alkaloids. Plant material derived different natural habitats cultures used for phytochemical analysis tests. composition alkaloids analyzed using chromatographic techniques (HPLC with DAD detection). results have shown that roots contained higher number amounts...
The root of Symphytum officinale L. is commonly used in folk medicine to promote the wound healing, reduce inflammation and treatment broken bones. objective our investigation was analyse extract from S. term its antioxidant activity effect on cell viability proliferation human skin fibroblast (HSF). Moreover, quantification main phenolics allantoin conducted using HPLC-DAD method. Five compounds were found: rosmarinic, p-hydroxybenzoic, caffeic, chlorogenic p-coumaric acid. DPPH, FRAP TPC...
This review provides a comprehensive and updated information on chalcones, presenting their sources, methods of identification properties putting emphasis applications in agricultural industry. Vast exploitation synthetic pesticides led to the weed pest resistance issue, causing economic loss but more importantly health risk due pesticide overuse. Because one aim European Green Deal 2030 is Farm Fork strategy which targets reducing by 50% use chemical 2030, push towards natural are...
Biofilm-related ocular infections can lead to vision loss and are difficult treat with antibiotics due challenges application increasing microbial resistance. In turn, the design testing of new synthetic drugs is a time- cost-consuming process. Therefore, in this work, for first time, we assessed vitro efficacy plant-based abietic acid molecule, both alone when introduced polymeric cellulose carrier, against biofilms formed by Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans...
Synthesis and investigation of antimicrobial activity 22 novel thiazoles selenazoles derived from dihydro-2H-thiopyran-4(3H)-one are presented. Additionally, anticonvulsant six derivatives is examinated. Among the derivatives, compounds 4a-f, 4i, 4k, 4 l, 4n, 4o-s 4v have very strong against Candida spp. with MIC = 1.95-15.62 μg/ml. In case 4o, 4r 4s, some strains isolated clinical materials, 0.98 to 15.62 4n-v found be active Gram-positive bacteria 7.81-62.5 The results screening reveal...
Due to certain differences in terms of molecular structure, isoquinoline alkaloids from Chelidonium majus engage various biological activities. Apart their well-documented antimicrobial potential, some phenanthridine and protoberberine derivatives as well C. extract present with anti-inflammatory cytotoxic effects. In this study, the LC-MS/MS method was used determine alkaloids, phenolic acids, carboxylic hydroxybenzoic acids. We investigated five individually tested (coptisine, berberine,...
Helicobacter pylori is a Gram-negative bacterium that colonizes the stomach of about 60% people worldwide. The search for new drugs with activity against H. now hotspot in effective and safe control this bacterium. Therefore, aim research was to determine antibacterial extracts from selected plants Papaveraceae family planktonic biofilm forms multidrug-resistant clinical strain using broad spectrum analytical vitro methods. It revealed among tested extracts, those obtained Corydalis...
The Chelidonium majus plant is rich in biologically active isoquinoline alkaloids. These alkaline polar compounds are isolated from raw materials with the use of acidified water or methanol; next, after alkalisation extract, they extracted using chloroform dichloromethane. This procedure requires toxic solvents. present study assessed possibility volatile natural deep eutectic solvents (VNADESs) for efficient and environmentally friendly extraction roots herb were subjected three times to...
Background: Pain is a major clinical and socioeconomic problem worldwide. The available therapies are not always effective often associated with the multiple adverse effects that reduce their application. Natural compounds an important group of pharmaceuticals may be used in pain management. We aimed to investigate analgesic activity sanguinarine–chelerythrine from Coptis chinensis. Methods: anti-inflammatory fraction C. chinensis extract (SC 5 10 mg/kg), sanguinarine (SAN 1 2 mg/kg)...
The excessive use of synthetic pesticides has not only resulted in increased resistance among weeds and pests, leading to significant economic loss but also raised serious health environmental concerns. Chalcones their derivatives, known for herbicidal, fungicidal, bactericidal, antiviral properties, are emerging as promising bio-based candidates. These naturally occurring compounds have long been recognized beneficial effects wide-range applications. However, limited concentration plants,...
The composition of volatile organic compounds emitted by in vitro shoots Agastache rugosa (Fischer & C.A. Meyer) O. Kuntze (Lamiaceae) was studied using headspace solid-phase microextraction–gas chromatography–mass spectrometry and compared to the those adult plants vitro-germinated seedlings. Shoot-tip explants were cultured on a solid MS medium supplemented with either 4.4 μM 6-benzyladenine (BA), 9.3 kinetin, or 0.45 thidiazuron 0.57 indole-3-acetic acid (IAA) 0.41 picloram. Shoot...
Inflammatory diseases are a common therapeutic problem and nonsteroidal anti-inflammatory drugs not deprived of side effects, which ulcerogenic activity is one the most frequent. The aim study was to evaluate sanguinarine-chelerythrine (SC) fraction Coptis chinensis its influence on integrity gastric mucosa. conducted sixty male rats randomly divided into six experimental groups: two control groups (a negative group CON positive CAR); three receiving an investigational C. (1, 5, 10 mg/kg...
A comprehensive study of the interactions human serum albumin (HSA) and α-1-acid glycoprotein (AAG) with two isoquinoline alkaloids, i.e., allocryptopine (ACP) protopine (PP), was performed. The UV-Vis spectroscopy, molecular docking, competitive binding assays, circular dichroism (CD) spectroscopy were used for investigations. results showed that ACP PP form spontaneous stable complexes HSA AAG, displaying a stronger affinity towards both proteins. Molecular docking studies revealed...
Plant in vitro culture is a feasible system for the testing influence of an environmental factor on accumulation and chemodiversity specialized metabolites, especially medicinal plants. Light temperature are among most important factors affecting physiology plant organisms but their specific metabolic pathways not completely understood. Here, we examined morphogenetic response, photosynthetic pigments content, lipid peroxidation level, DPPH radical scavenging activity, production volatile...
Effects of non-specific ethylene biosynthesis inhibitors: salicylic acid (SA) and aminoethoxyvinylglycine (AVG), specific inhibitors binding to receptors: 1-methylcyclopropene (1-MCP) 2,5-norbornadiene (NBD) applied during proliferation differentiation phases indirect somatic embryogenesis (SE) Medicago sativa L. cv. Rangelander on embryogenic suspension growth, embryo production, development, ability germinate convert were studied. Application SA AVG alone or together at concentrations from...
The accurate profiling of Agastache rugosa phenolic compounds is an indispensable step toward better understanding the medicinal properties species. applied method based on coupling fast centrifugal partition chromatography and UPLC–qTOF–MS alternative rapid for separation preliminary purification included in crude extract can facilitate detection minor compounds. Samples were prepared by extraction leaves inflorescences with methanol, dichloromethane, ethyl acetate. Polyphenolic separated...
Abstract Plant cell and organ cultures are potential sources of valuable secondary metabolites that can be used as food additives, nutraceuticals, cosmeceuticals, pharmaceuticals. Phytochemical biosynthesis in various vitro plant cultures, contrast to planta, is independent environmental conditions free from quality fluctuations. Pharmaceutical application biotechnology interest almost all departments the Faculty Pharmacy Institute Pharmacology Poland with a botanical profile (Pharmaceutical...