A5002196082- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Steroid Chemistry and Biochemistry
- Fungal Biology and Applications
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
City University of Hong Kong
2021-2023
A biosynthetic gene cluster for the bioactive fungal sesterterpenoids variecolin (1) and variecolactone (2) was identified in Aspergillus aculeatus ATCC 16872. Heterologous production of 1 2 achieved oryzae by expressing sesterterpene synthase VrcA cytochrome P450 VrcB. Intriguingly, replacement VrcB with homologous P450s from other terpenoid pathways yielded three new analogues (5–7). Analysis compounds' anticancer activity vitro vivo revealed that although 5 had comparable activities,...
The biosynthetic gene cluster of fungal bioactive sesterterpenoids, variecolin (1) and variecolactone (2), was identified in Aspergillus aculeatus ATCC 16872. Heterologous production 1 2 achieved oryzae by expressing the sesterterpene synthase VrcA cytochrome P450 VrcB. Intriguingly, replacement VrcB with homologous P450s from other terpenoid pathways yielded three new analogues, one which exhibited potent anticancer activity comparable to that 1.
The biosynthetic gene cluster of fungal bioactive sesterterpenoids, variecolin (1) and variecolactone (2), was identified in Aspergillus aculeatus ATCC 16872. Heterologous production 1 2 achieved oryzae by expressing the sesterterpene synthase VrcA cytochrome P450 VrcB. Intriguingly, replacement VrcB with homologous P450s from other terpenoid pathways yielded three new analogues, one which exhibited potent anticancer activity comparable to that 1.