To improve DNA resolution of fluorescence in situ hybridization we have adapted a nuclear extraction technique, resulting highly extended loops arranged around the matrix halo-like structure. In signals from alphoid and cosmid DNAs appear as beads-on-a-string, which, according to preliminary experiments, results association individual probe fragments. By multicolor hybridizations been able determine relative map position easily detect 10 kb overlap between clones, each which shows linear...

Rat liver sinusoidal endothelial cells (LEC) contain fenestrae, which are clustered in sieve plates. Fenestrae control the exchange of fluids, solutes and particles between blood space Disse, at its back side is flanked by microvillous surface parenchymal cells. The LEC can optimally be imaged scanning electron microscopy (SEM), SEM images used to study dynamic changes fenestrae comparing fixed specimens subjected different experimental conditions. Unfortunately, allows only investigation...

AbstractThe establishment of a cross-discipline peer consultation group in regional Australia is described. A research project, which explored the impact on participants, using pre-group and post-group evaluation surveys along with data from an externally facilitated session discussed. Key findings reveal value access to varied modes supervision; importance supervision not feeling like being subjected surveillance; contribution that positive experiences have for our participants' well-being....

Cationic liposomes are commonly used for transfection of plasmids into mammalian cells, while microspheres have been traditionally selective delivery anticancer agents tumor vasculature. We developed a novel vector, comprised cationic electrostatically bound to ion-exchange (termed 'microplex') targeted gene therapy solid tumors. The modes tested in rat model were free plasmids, microspheres, liposomes, or the combination vector. greatest amount chloramphenicol acetyltransferase (CAT)...

Summary Fluorescence in situ hybridization on human metaphase chromosomes is detected by near‐field scanning optical microscopy. This combination of cytochemical and probe techniques enables the localization identification several fluorescently labelled genomic DNA fragments a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1. 77. p1–79 plasmid α‐spectrin. The signals very well resolved fluorescence images, while exact location can be correlated...

The acute genoprotective effect of Panax quinquefolius (American ginseng) has been investigated. experiment was carried out to explore the DNA protective after a single dose American ginseng tea bag infusion. Fourteen subjects (6 males and 8 females) were recruited in this study. Seven them (3 4 asked drink cup freshly prepared infusions. Water taken by remaining as control group. Blood samples both groups before 2 h post-ingestion. blood challenged with ultraviolet B irradiation followed...

Some traditional Chinese medicinal seeds and fruits are well known for their antioxidant properties. This research aims to investigate whether Fructus Lycii, Schisandrae Chinensis, Ligustri Lucidi Semen Cuscutae protect DNA from oxidant challenge by hydrogen peroxide (H(2)O(2)). The standard comet assay was used assess the genoprotective effect of these herbs. Blood taken three healthy adults, aged 36 42. Lymphocytes were isolated treated with different concentrations aqueous herbal...

Grape seed extracts (GSEs) possess a broad spectrum of antioxidative properties that protects various cells from free radicals and oxidative stress. In this study, the genoprotective effect GSE on human lymphocytic DNA was studied using standard lysed cell comet assays. Lymphocytes 5 healthy subjects were pretreated with in different concentrations. The assays performed treated, untreated, challenged, unchallenged parallel. Cells then subjected to an oxidant challenge induced 5-min exposures...

Abstract Adenoviral vectors have been commonly used in gene therapy protocols but the success of their use is often limited by induction host immunity to vector. Following exposure adenoviral vector, adenoviral-specific neutralising antibodies are produced, which limits further administration. This study examines effectiveness a novel combination microspheres and liposomes for shielding adenovirus from an in-vitro setting. We show that effective protection antibody conjugation these...

The potential acute genoprotective effect of orange juice supplementation was investigated. Six healthy subjects (aged 33 to 60 years; 3 women and men) were asked drink 400 mL commercial juice, which contained 100 mg vitamin C 40.8 g sugar. Venous blood (2 mL) taken before 2 h after ingestion (test trial). A week later, the repeat trial by drinking water with glucose (control Lymphocytes isolated from samples underwent comet assay on day collection. Pre- postingestion DNA damage scores...

Plasmid DNA binding to cationic liposomes and the ability bind these liposomes, both with without complexed plasmid DNA, cation-exchange microspheres were examined. The two plasmids tested pCMV-CAT pRcCMV-p53. Commercial Lipofectin, Lipofectace, Lipofectamine, three formulation ratios of dimethyldioctadecyl ammonium bromide (DDAB):phosphatidylcholine DDAB:dioleoylphosphatidyl ethanolamine evaluated. empty onto increased release from decreased increasing ratio cationic:neutral lipid. Of all...

Scanning probe microscopy (SPM) techniques, including atomic force (AFM) and scanning tunnelling (STM), have revolutionized our understanding of molecule–surface interactions. The high resolution versatility SPM techniques helped elucidate the morphology adsorbed surfactant layers, facilitated study electronically conductive single molecules biomolecules connected to metal substrates, allowed direct observation real-time processes such as in situ DNA hybridization drug–cell These examples...

Context and objective: Scientific evidence has shown Coriolus versicolor (L. ex Fr.) Quel (also known as Yunzhi) the role of immunomodulator in therapeutic effect. The aim this vitro study was to investigate antioxidative effect Yunzhi explore mechanisms behind its DNA protection.Materials methods: Commercial extract dissolved water diluted five concentrations (101–105 μg/L) with appropriate buffers. Lymphocytes harvested from three healthy subjects were incubated for 30 min. Cells then...

The ability to culture neurons from horses may allow further investigation into equine neurological disorders. In this study, we demonstrate the generation of induced neuronal cells adipose-derived stem (EADSCs) using a combination lentiviral vector expression transcription factors Brn2, Ascl1, Myt1l (BAM) and NeuroD1 defined chemical induction medium, with βIII-tubulin–positive displaying distinct morphology rounded compact cell bodies, extensive neurite outgrowth, branching processes....

In this study we investigated the possibility of imaging internal cellular molecules after cytochemical detection with atomic force microscopy (AFM). To end, rat 9G and HeLa cells were hybridized haptenized probes for 28S ribosomal RNA, human elongation factor mRNA cytomegalovirus immediate early antigen mRNA. The hybrids subsequently detected a peroxidase-labelled antibody visualized 3.3'-diaminobenzidine (DAB). influence various scanning conditions on cell morphology visibility signal was...