A5079885280- Global Cancer Incidence and Screening
- RNA and protein synthesis mechanisms
- Innovations in Medical Education
- CRISPR and Genetic Engineering
- Nursing Roles and Practices
- Data-Driven Disease Surveillance
- Global Health Workforce Issues
- Medical Education and Admissions
- Economic and Financial Impacts of Cancer
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Primary Care and Health Outcomes
- RNA Research and Splicing
- Force Microscopy Techniques and Applications
- Blood Pressure and Hypertension Studies
- Diversity and Career in Medicine
- Health and Conflict Studies
- Ethics in Clinical Research
- Healthcare Systems and Technology
- Health Systems, Economic Evaluations, Quality of Life
- Down syndrome and intellectual disability research
- Frailty in Older Adults
- AI in cancer detection
George Washington University
2008-2025
QB3
2025
University of California, Berkeley
2012-2025
Royal College of Surgeons in Ireland
2021-2025
University of Colorado Anschutz Medical Campus
2023-2025
Meharry Medical College
2025
High Point University
2025
The University of Texas Southwestern Medical Center
2023-2025
Physician Assistant Education Association
2018-2025
University of the West Indies
2025
Abstract Type II CRISPR endonucleases are widely used programmable genome editing tools. Recently, CRISPR-Cas systems with highly compact nucleases have been discovered, including Cas9d (a type II-D nuclease). Here, we report the cryo-EM structures of a nuclease (747 amino acids in length) multiple functional states, revealing stepwise process DNA targeting involving conformational switch REC2 domain insertion. Our provide insights into intricately folded guide RNA which acts as structural...
Mycobacteriophages are viruses that infect mycobacterial hosts such as Mycobacterium smegmatis and tuberculosis. All mycobacteriophages characterized to date dsDNA tailed phages, have either siphoviral or myoviral morphotypes. However, their genetic diversity is considerable, although sixty-two genomes been sequenced comparatively analyzed, these likely represent only a small portion of the mycobacteriophage population at large. Here we report isolation, sequencing comparative genomic...
A detailed understanding of tRNA/mRNA translocation requires measurement the forces generated by ribosome during this movement. Such measurements have so far remained elusive and, thus, little is known about relation between force and how reflects on its mechanism regulation. Here, we address these questions using optical tweezers to follow translation individual ribosomes along single mRNA molecules, against an applied force. We find that rates depend exponentially force, with a...
Abstract Background: Patient Navigation (PN) originated in Harlem as an intervention to help poor women overcome access barriers timely breast cancer treatment. Despite rapid, nationally widespread adoption of PN, empirical evidence on its effectiveness is lacking. In 2005, National Cancer Institute initiated a multicenter PN Research Program (PNRP) measure for several cancers. The George Washington Institute, project participant, established District Columbia (DC)-PNRP determine PN's...
Ribosome biogenesis is a complex multistep process that involves alternating steps of folding and processing pre-rRNAs in concert with assembly ribosomal proteins. Recently, there has been increased interest the roles proteins eukaryotic ribosome vivo, focusing primarily on their function pre-rRNA processing. However, much less known about participation formation rearrangement preribosomal particles as they mature to functional subunits. We have studied L7 L8, which are required for same...
Abstract Protein folding can begin co-translationally. Due to the difference in timescale between and synthesis, co-translational is thought occur at equilibrium for fast-folding domains. In this scenario, kinetics of stalled ribosome-bound nascent chains should match real time. To test if assumption true, we compare a ribosome-bound, multi-domain calcium-binding protein different points translation with chain as it being synthesized real-time, via optical tweezers. On ribosomes, misfolded...
CRISPR-associated (Cas) transposases (CAST) are RNA-guided systems capable of programmable integration large segments DNA without creating double-strand breaks. Engineered Cascade CAST function in human cells but challenging to deploy due the complexity targeting components. Unlike Cascade, which require three Cas proteins, type V-K a single Cas12k effector for targeting. Here, we show that compact from uncultivated microbes repurposable into genome cells. Engineering nuclear localization...
Understanding changes in global oceanic precipitation remains challenging due to limitations current observational datasets and model deficiencies, particularly the representation of cloud properties within regions. To address this, we examined climatologies trends between 2001 2020 using a collection 27 state-of-the-art satellite reanalysis available on uniform daily 1°×1° resolution from Frequent Rainfall Observations Grids (FROGS) database. The results showed...
Abstract This paper focuses on the impact of artificial intelligence (AI) in physician assistant/associate (PA) education based upon results from a national survey PA faculty conducted by Education Association early 2024. is first its kind to assess perspectives topic. It adds growing body knowledge related AI across all contemporary business, social, and educational activities. A containing Likert ranking questions open-ended comments was sent listed database (n = 4338). response rate 14.8%...
Understanding the intricate folding process of proteins and characterizing intermediates they populate en route to their native state remain challenging despite remarkable accuracy achieved through in silico approaches for predicting protein structures. Here, we replaced conventional flexible double-stranded DNA handle force transducers with solid DNA-origami bundles conduct single-molecule force-spectroscopy studies on calerythrin, a compact multidomain calcium-binding globular protein. The...
Abstract Programmable, RNA-guided nucleases are diverse enzymes that have been repurposed for biotechnological applications. However, to further expand the therapeutic application of these tools there is a need targetable systems small enough be delivered efficiently. Here, we mined an extensive genome-resolved metagenomics database and identified families uncharacterized RNA-guided, compact (between 450 1,050 aa). We report Cas9d, new CRISPR type II subtype, contains Zinc-finger motifs high...
Delays in follow-up after breast cancer screening contribute to disparities outcomes. The objective of this research was determine the impact race/ethnicity and health insurance on diagnostic time, defined as number days from suspicious finding resolution.
Abstract As an ever-increasing number of physician assistant (PA) programs moves toward holistic admissions, a better understanding how to achieve their stated admission goals becomes more important. With the June 2023 US Supreme Court decision effectively ending affirmative action in higher education, navigating admissions is now even greater challenge. In this article, PA Education Association's Presidents Commission offers guide for use implementing at institutions and key considerations....
The present research examined the validity of self-report versus informant-report in relation to a performance-based indicator adult ADHD.Archival data from 118 participants (52 males, 66 females) were used compare Conners' Adult ADHD Rating Scale-Self-Report: Long Format (CAARS-S:L) and Scale-Observer Report: (CAARS-O:L) with discrepancy scores calculated between Wechsler Intelligence Scale-Third Edition (WAIS-III) Verbal Comprehension Index - Working Memory (VCI WMI)...
<h3>Background:</h3> Resource stewardship is being increasingly recognized as an essential competency for physicians, but medical schools are just beginning to integrate this into education. We describe the evaluation of Choosing Wisely Canada9s Students and Trainees Advocating Stewardship (STARS) campaign, a student-led campaign advance resource education in across Canada. <h3>Methods:</h3> evaluated 6 months after its launch, November 2015. STARS students were administered telephone survey...
The treatment of patients with acute and chronic pain not attributed to cancer or end-of-life conditions is a challenge for many clinicians. Although CDC guidelines that focus on the primary care setting have provided critical recommendations, evidence-based guidance lacking optimal duration opioid postoperative in specialty settings. Over last 2 decades, liberal use opioids has resulted unintended consequences, including dependence abuse, illicit distribution legally illegally prescribed...
The current pipeline of physician assistant (PA) school applicants reflects the future workforce profession, which is why admissions process with all its components and variables so important. Many studies have shown that a represents patients it cares for leads to improved health outcomes, especially among underrepresented minority populations. Yet, PA programs made little progress over past 2 decades in increasing diversity matriculants graduates. As our collective responsibility...
Type II Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 nucleases have been extensively used in biotechnology and therapeutics. However, many applications are not possible owing to the size, targetability, potential off-target effects associated with currently known systems. In this study, we identified thousands of CRISPR type effectors by mining an extensive, genome-resolved metagenomics database encompassing hundreds microbial genomes. We developed a...
Iron-TAML activators of peroxides are functional mimics peroxidase and short-circuited cytochrome P450 enzymes that perform numerous transformations appear by all measures to date be fully life cycle compatible with the environment. Here we show how design same catalytic chemistry without direct addition H2O2, thereby removing need transport store hydrogen peroxide. In this new approach, dioxygen is reduced in situ D-glucose presence glucose oxidase (GO) from Aspergillus niger. The resulting...
Abstract The physician assistant (PA) profession has successfully flourished for over 50 years, demand graduates remains strong, and there is a wealth of research on the quality care that PA provide. Nevertheless, health education are constantly changing, we must change with them. We continually scan horizon consider how best to adapt current issues like clinical sites crisis, as well evolutions in technology, pedagogy, our students themselves. Physician Assistant Education Association...
Cas12a enzymes are quickly being adopted for use in a variety of genome-editing applications. These programmable nucleases part adaptive microbial immune systems, the natural diversity which has been largely unexplored. Here, we identified novel families Type V-A CRISPR through large-scale analysis metagenomes collected from complex environments, and developed representatives these systems into gene-editing platforms. The display extensive protein variation can be programmed by single-guide...