A5084099446- Glycosylation and Glycoproteins Research
- Proteoglycans and glycosaminoglycans research
- Electrolyte and hormonal disorders
- Monoclonal and Polyclonal Antibodies Research
- Biochemical and Structural Characterization
- Peptidase Inhibition and Analysis
- Enzyme Production and Characterization
- Toxoplasma gondii Research Studies
- Clinical Nutrition and Gastroenterology
- Acupuncture Treatment Research Studies
- Ovarian function and disorders
- Protease and Inhibitor Mechanisms
- Advancements in Transdermal Drug Delivery
- Effects and risks of endocrine disrupting chemicals
- Plant Surface Properties and Treatments
- Advanced Drug Delivery Systems
- Carcinogens and Genotoxicity Assessment
- Cancer Research and Treatments
- Sperm and Testicular Function
- Genetic factors in colorectal cancer
- Potassium and Related Disorders
- Medical Imaging and Pathology Studies
- Bone health and treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Muscle metabolism and nutrition
Kyushu University
2009-2024
Daiichi-Sankyo (Japan)
2018-2023
Otsuka Pharmaceutical (Spain)
1989-2006
Otsuka (Japan)
2000-2006
Heparan sulfate is a ubiquitous glycosaminoglycan in the extracellular matrix of most animals. It interacts with various molecules and exhibits important biological functions. K5 antigen produced by Escherichia coli strain linear polysaccharide N-acetylheparosan consisting GlcUA beta1-4 GlcNAc alpha1-4 repeating disaccharide, which forms backbone heparan sulfate. Region 2, located center K5-specific gene cluster, encodes four proteins, KfiA, KfiB, KfiC, KfiD, for biosynthesis polysaccharide....
Herein, we report a transdermal patch prepared using an ionic liquid-based solid in oil (IL-S/O) nanodispersion and pressure-sensitive adhesive (PSA) to deliver the macromolecular antigenic protein, ovalbumin (OVA). The IL-S/O PSA were first mixed at equal weight ratio, then coated onto release liner, covered with support film. To evaluate effect of PSA, three types PSAs, DURO-TAK 87-4098, 87-4287, 87-235A, used obtain corresponding patches SP-4098, SP-4287, SP-235A, respectively....
The fine structures of Fc N-glycan modulate the biological functions and physicochemical properties antibodies. By remodeling to obtain a homogeneous glycoform or chemically modified glycan, antibody characteristics can be controlled modified. Such achieved by transglycosylation reactions using mutant endoglycosidase from Streptococcus pyogenes (Endo-S) glycan oxazoline. In this study, we generated improved mutants Endo-S introducing additional mutations D233Q mutant. Notably, D233Q/Q303L,...
Although the toxic effects of citrate including hemodynamic and cardiovascular changes result from a decrease in ionized calcium levels serum due to chelating action, these on blood coagulation have not yet been fully clarified. The present study examines whether affect whole clotting time rats using test tube method which is administered by rapid intravenous infusion. Citrate was infused via tail vein into 10 at 3, 4 or 5 mmol/kg/hr for 1 hr, then time, were determined. Whole did...
Abstract Mutations in activin receptor-like kinase 2 (ALK2) can cause the pathological osteogenic signaling seen some patients with fibrodysplasia ossificans progressiva and other conditions such as diffuse intrinsic pontine glioma. Here, we report that intracellular domain of wild-type ALK2 readily dimerizes response to BMP7 binding drive signaling. This is pathologically triggered by heterotetramers type II receptor kinases mutant forms, which form dimers A binding. We develop a blocking...
Chondroitin lyases have been known as pathogenic bacterial enzymes that degrade chondroitin. Recently, baculovirus envelope protein ODV‐E66 was identified the first reported viral chondroitin lyase. has low sequence identity with at <12%, and unique characteristics reflecting life cycle of baculovirus. To understand ODV‐E66's structural basis, crystal structure determined it found fold resembled polysaccharide lyase 8 proteins catalytic residues were also conserved. This enabled...
Abstract Proteases are one of attractive therapeutic targets to play key roles in pharmacological action. There many protease inhibitors nature, and most them structurally have cystine knot motifs. Their structures favorable for recognition active pockets proteases, leading the potent inhibition. However, they also drawbacks, such as broad cross-reactivity, on application. To create proteins derived from a disulfide-rich scaffold, we selected human serine inhibitor Kazal type 2 (SPINK2)...
Matrix metalloproteinases (MMPs) contribute to many physiological and pathological phenomena via the proteolysis of extracellular matrix components. Specific blocking active site each MMP sheds light on its particular role. However, it remains difficult acquire an active-site inhibitor with high specificity for only target due highly conserved structure around MMPs. Recently, we reported that potent specific inhibitors serine proteases were obtained from our proprietary engineered protease...
The toxic effects of i.v. administration N-acetyl-l-cysteine (NAC), a component parenteral nutrition solutions, on fertility and embryonic development were investigated in SD male female rats at doses 100, 300, 1000 mg kg-1 day-1. Infertility was observed females the 1000-mg/kg group throughout period from before mating to embryogenesis. No effect NAC reproductive ability seen. oocytes embryos assessed morphologically clarify cause NAC. unfertilized (UO) recovered ampullae uterine tubes...
Baculovirus envelope protein ODV-E66 (67-704), in which the N-terminal 66 amino acids are truncated, is a chondroitin lyase. It digests and 6-sulfate efficiently, but does not digest 4-sulfate. This unique characteristic useful for preparation of specific oligosaccharides investigation mechanism baculovirus infection. (67-704) was crystallized; crystal diffracted to 1.8 Å resolution belonged space group P6(2) or P6(4), with unit-cell parameters = b 113.5, c 101.5 Å. One molecule assumed be...
We previously reported infertility in female rats that received N-acetyl-L-cysteine (NAC) intravenously at a dosage of 1000 mg/kg/day. Unfertilized oocytes and gestation day 1 2 embryos were assessed morphologically, the results suggested absence or thinning zona pellucida (ZP) is related to infertility. However, morphological characteristics before ovulation recovery from effects NAC not clarified. In present study, ovarian follicles histopathologically examined reproductive function was...
Chronological changes in hemodynamics and histopathological lesions of the brain were investigated rats given a single dose hypertonic glucose solution (osmotic ratio: 6.5) intravenously or intraperitoneally. After administration, dehydration developed rapidly markedly but recovery was noted on following day. An excess dosage caused cerebral with meningeal hemorrhage showing nervous signs. Shrinkage neurons, loosening vacuolation ground substance swollen astrocytes (spongy appearance) seen...