A5053434259- Bacterial Infections and Vaccines
- Escherichia coli research studies
- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Toxoplasma gondii Research Studies
- Autophagy in Disease and Therapy
- Adipose Tissue and Metabolism
- Cellular Mechanics and Interactions
- Metabolomics and Mass Spectrometry Studies
- Influenza Virus Research Studies
- Cell Image Analysis Techniques
- Bacterial Genetics and Biotechnology
- Mitochondrial Function and Pathology
- 3D Printing in Biomedical Research
- Muscle Physiology and Disorders
- Immune Response and Inflammation
- Sirtuins and Resveratrol in Medicine
- Lipid Membrane Structure and Behavior
Czech Academy of Sciences, Institute of Microbiology
2006-2024
Czech Academy of Sciences
2006-2024
Environmental stresses inducing translation arrest are accompanied by the deposition of translational components into stress granules (SGs) serving as mRNA triage sites. It has recently been reported that, in Saccharomyces cerevisiae, formation SGs occurs a result prolonged glucose starvation. However, these did not contain eIF3, one hallmarks mammalian SGs. We have analyzed effect robust heat shock on distribution eIF3a/Tif32p/Rpg1p and showed that it results eIF3a accumulations containing...
Adenylate cyclase toxin (CyaA or ACT) is a key virulence factor of pathogenic Bordetellae. It penetrates phagocytes expressing the alpha(M)beta(2) integrin (CD11b/CD18, Mac-1 CR3) and paralyzes their bactericidal capacities by uncontrolled conversion ATP into signaling molecule, cAMP. Using pull-down activity assays transfections with mutant Rho family GTPases, we show that cAMP CyaA causes transient selective inactivation RhoA in mouse macrophages absence detectable activation Rac1, Rac2,...
The Bordetella adenylate cyclase-hemolysin (CyaA, ACT, or AC-Hly) is a multifunctional toxin. Simultaneously with promoting calcium ion entry, CyaA delivers into host cells an cyclase enzyme (AC) and permeabilizes cell membrane by forming small cation-selective pores. Indirect evidence suggested that these two activities were accomplished different membraneinserted conformers, one acting as AC-delivering monomer the other uncharacterized poreforming oligomer. We tested this model directly...
Abstract Adenylate cyclase toxin (CyaA) of Bordetella pertussis penetrates the membrane eukaryotic cells, producing high levels intracellular cAMP, as well hemolysis that results from formation cation‐selective channels in membrane. Using several microscopical approaches we studied effects CyaA action on morphology sheep erythrocytes during early phases preceding lysis and examined localization molecules within erythrocyte induced a cascade morphological changes erythrocytes, such shrinkage,...
Translationally controlled tumor protein (TCTP) is a multifunctional and highly conserved from yeast to humans. Recently, its role in non-selective autophagy has been reported with controversial results mammalian human cells. Herein we examine the effect of Mmi1, ortholog TCTP, on budding Saccharomyces cerevisiae, well-established model system monitor autophagy. We induced by nitrogen starvation or rapamycin addition measured using Pho8Δ60 GFP-Atg8 processing assays WT, mmi1Δ,...
Abstract Mitochondrial morphology is an important parameter of cellular fitness. Although many approaches are available for assessing mitochondrial in mammalian cells, only a few technically demanding and laborious methods yeast cells. A robust, fully automated user-friendly approach that would allow (1) segmentation tubular spherical mitochondria the Saccharomyces cerevisiae from conventional wide-field fluorescence images (2) quantitative assessment lacking. To address this, we compared...