A5058386295- Platelet Disorders and Treatments
- Blood properties and coagulation
- Venous Thromboembolism Diagnosis and Management
- COVID-19 Clinical Research Studies
- Cell Adhesion Molecules Research
- Blood disorders and treatments
- Thyroid Disorders and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Blood groups and transfusion
- Inflammatory Biomarkers in Disease Prognosis
- Complement system in diseases
- Heparin-Induced Thrombocytopenia and Thrombosis
- Antiplatelet Therapy and Cardiovascular Diseases
- Hormonal Regulation and Hypertension
- Stress Responses and Cortisol
- Vascular Malformations and Hemangiomas
- Nitric Oxide and Endothelin Effects
- Extracellular vesicles in disease
- Sphingolipid Metabolism and Signaling
- Lymphatic System and Diseases
- Erythrocyte Function and Pathophysiology
- Renal Diseases and Glomerulopathies
- Central Venous Catheters and Hemodialysis
- Polysaccharides and Plant Cell Walls
- Birth, Development, and Health
Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology
2020-2024
Center for Theoretical Problems of Physicochemical Pharmacology
2018-2024
Institute of Biochemical Physics NM Emanuel
2020-2022
Russian Academy of Sciences
2020-2022
Ministry of Health of the Russian Federation
2020-2022
Lomonosov Moscow State University
1998-2021
Moscow State University
2014-2021
Institute of Biomedical Problems
2015-2018
State Research Center of the Russian Federation
2015
Mayo Clinic in Arizona
2012
IntroductionDefects of platelet functional responses in COVID-19 were reported, but their origin and pathophysiological significance are unclear. The objective this study was to characterize the thrombocytopathy COVID-19.Materials methodsAnalysis activation by flow cytometry aggregometry 46 patients with confirmed different severity (non-ICU, ICU, ECMO) over course hospitalization alongside plasma coagulation, inflammatory markers (CRP, fibrinogen, NETosis assays smears) performed.Results...
Although reversible platelet aggregation observed in response to ADP stimulation the presence of calcium is a well-known phenomenon, its mechanisms are not entirely clear. To study them, we developed simple kinetic mass-action-law-based mathematical model use it combination with experiments. Light transmission aggregometry (LTA) induced by was performed for platelet-rich plasma or washed platelets using both conventional light and aggregate size monitoring method based on optical density...
Immune thrombocytopenia (ITP) is an autoimmune condition primarily induced by the loss of immune tolerance to platelet glycoproteins. Here we develop a novel flow cytometry approach analyze integrin αIIbβ3 functioning in ITP comparison with Glanzmann thrombasthenia (GT) (negative control) and healthy pediatric donors (positive control). Continuous Fura-Red-loaded platelets from whole hirudinated blood was used for characterization responses conventional activators. Calcium levels fibrinogen...
Abstract Platelets are anucleate blood cells with reported roles in hemostasis and immune responses, which possess a functional receptor for bacterial lipopolysaccharides (LPSs), the well-known inducers of inflammation. However, LPSs effects on platelets contradictory. Here we aim to investigate mechanisms platelet functioning presence LPS find cause discrepancy previously published data. Cell activity was analyzed by flow cytometry, western blotting, aggregometry. Thrombus growth assessed...
Thromboinflammation is caused by mutual activation of platelets and neutrophils. The site thromboinflammation determined chemoattracting agents release endothelium, immune cells, platelets. Impaired neutrophil chemotaxis contributes to the pathogenesis Shwachman-Diamond syndrome (SDS). In this hereditary disorder, neutrophils are known have aberrant chemoattractant-induced F-actin properties. Here, we aim determine whether could be analyzed using our previously developed ex vivo assay...
Kaposiform hemangioendothelioma (KHE) is a rare vascular tumor of infancy that commonly associated with life-threatening thrombocytopenic condition, Kasabach-Merritt phenomenon (KMP). Platelet CLEC-2, podoplanin interaction considered the key mechanism platelet clearance in these patients. Here, we aimed to assess functionality such Three groups 6 9 children were enrolled: group A KHE/KMP without hematologic response (HR) therapy; B HR; and C healthy children. was assessed by continuous end...
Abstract Background The process of thrombus formation is thought to involve interactions between platelets and leukocytes. Leukocyte incorporation into growing thrombi has been well established in vivo, a number properties platelet-leukocyte critical for have characterized vitro thromboinflammatory settings clinical relevance. activity can be impaired distinct hereditary acquired disorders immunological nature, among which Wiskott-Aldrich Syndrome (WAS). However, more quantitative...
To date, fewer than 20 patients have been identified as having germline biallelic mutations in the coronin-1A gene (CORO1A) and its protein with clinical features of combined immunodeficiency characterized by T-cell lymphopenia ranging from severe phenotype to mild phenotype, recurrent infections, lymphoproliferative disorders. However, effects CORO1A disruption on actin-dependent functions primary cells not fully delineated.We sought characterize underlying defects cellular a female patient...
Abstract Immune thrombocytopenia (ITP) is believed to be associated with platelet function defects. However, their mechanisms are poorly understood, in particular regard differences between ITP phases, patient age, and therapy. We investigated bleeding children either persistent or chronic ITP, without romiplostim The study included 151 of whom 56 had disease duration less than 12 months (grouped together as acute/persistent) 95 were chronic. Samples 57 healthy used controls, while 5...
Small, non-nuclear cells, platelets, are primarily designed to form aggregates when blood vessels damaged, stopping bleeding. To perform this function, platelets can implement several functional responses induced by various agonists and coordinated a complex network of intracellular signaling triggered dozen different receptors. This review, the second in series, describes known pathways platelet receptors response canonical rare agonists. Particular focus will be on interaction points...
The process of clustering plasma membrane receptors in response to their agonist is the first step signal transduction. rate and size clusters determine further cell responses. Here we aim demonstrate that a simple 2-differential equation mathematical model capable quantitative description kinetics 2D or 3D cluster formation various processes. Three models based on mass action were considered compared with each other by ability describe experimental data GPVI CR3 receptor (2D) albumin...
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One of the most dangerous features new coronavirus infection caused by SARS-CoV-2 virus is tendency hemostasis system patients to excessive thrombus formation. Among possible causes this pathology, both activation vascular endothelial cells, leading exposure tissue factor these and direct plasma were named. Besides, there a significant change in platelet responses activation, which not accompanied thrombocytopenia. The mechanism dysfunction rather controversial. On one hand, are suggestions...
Platelet activating receptor CLEC-2 has been identified on platelet surface a decade ago. The only confirmed endogenous agonist is podoplanin. Podoplanin transmembrane protein expressed by lymphatic endothelial cells, reticular fibroblastic cells in lymph nodes, kidney podocytes and of certain tumors. Association with podoplanin involved processes embryonic development (blood-lymph vessel separation angiogenesis), maintaining vascular integrity small vessels during inflammation prevention...
Aim . To investigate the state of platelet and plasma components hemostasis in patients with myocardial infarction non-obstructive coronary arteries (MINOCA). Material methods The study included 42 non-ST-segment elevation (NSTEMI): MINOCA (n=24) MI-CAD (n=18). Platelet aggregation ability response to activation was evaluated using Solar AP2110 LASCA aggregometers. functional activity calcium signaling were assessed flow cytometry methods. component hemostasis, addition routine coagulation...