A5046865187- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Methane Hydrates and Related Phenomena
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Marine Biology and Ecology Research
- Immune Cell Function and Interaction
- Hydrocarbon exploration and reservoir analysis
- Geology and Paleoclimatology Research
- Protist diversity and phylogeny
- Glycosylation and Glycoproteins Research
- Marine Bivalve and Aquaculture Studies
- Cell Adhesion Molecules Research
- Microbial Metabolic Engineering and Bioproduction
- Coral and Marine Ecosystems Studies
- Algal biology and biofuel production
- Crustacean biology and ecology
- Rare-earth and actinide compounds
- Vibrio bacteria research studies
- Iron-based superconductors research
- Microbial Fuel Cells and Bioremediation
- Immune Response and Inflammation
- Atmospheric and Environmental Gas Dynamics
- Photosynthetic Processes and Mechanisms
- Fermentation and Sensory Analysis
Ichimaru Pharcos (Japan)
2023-2025
Japan Agency for Marine-Earth Science and Technology
2013-2024
National Institutes of Natural Sciences
2022-2024
The Exploratory Research Center on Life and Living Systems
2022-2024
Kyoto University
2006-2024
Living Systems (United States)
2023
Institute of Natural Science
2023
Kyowa Kirin (Japan)
1995-2021
Aichi Medical University
2020
Fujifilm (Japan)
2017-2020
We have developed a technique for cultivation of chemolithoautotrophs under high hydrostatic pressures that is successfully applicable to various types deep-sea chemolithoautotrophs, including methanogens. It based on glass-syringe-sealing liquid medium and gas mixture used in conjunction with butyl rubber piston metallic needle stuck into rubber. By using this technique, growth, survival, methane production newly isolated, hyperthermophilic methanogen Methanopyrus kandleri strain 116 are...
The deep subseafloor biosphere is among the least-understood habitats on Earth, even though huge microbial biomass therein plays an important role for potential long-term controls global biogeochemical cycles. We report here vertical and geographical distribution of microbes their phylogenetic diversities in deeply buried marine sediments Pacific Ocean Margins. During Drilling Program Legs 201 204, we obtained sediment cores from Peru Cascadia Margins that varied with respect to presence...
To date 142 species have been described in the Vibrionaceae family of bacteria, classified into seven genera; Aliivibrio, Echinimonas, Enterovibrio, Grimontia, Photobacterium, Salinivibrio and Vibrio. As vibrios are widespread marine environments show versatile metabolisms ecologies, these bacteria recognized as one most diverse important heterotrophic bacterial groups for elucidating correlation between genome evolution ecological adaptation. However, on basis 16S rRNA gene phylogeny, we...
13,16-Dimethyl octacosanedioic acid (iso-diabolic acid) is a major membrane-spanning lipid of subdivisions (SDs) 1, 3 and 4 the Acidobacteria, highly diverse phylum within Bacteria. It has been suggested that these lipids are potential building blocks for orphan bacterial glycerol dialkyl tetraethers (GDGT) occur widely in variety environmental settings. Here, we expand knowledge on occurrence iso-diabolic Acidobacteria by examining composition six strains belonging to SDs 6, 8, 10, 23 not...
Abstract IL-4 has been shown to be involved in the accumulation of leukocytes, especially eosinophils, at sites inflammation by acting on vascular endothelial cells. To identify novel molecules IL-4-dependent eosinophil extravasation, cDNA prepared from HUVEC stimulated with was subjected differential display analysis, which revealed a CC chemokine designated as eotaxin-3. The human eotaxin-3 gene localized chromosome 7q11.2, unlike most other genes. predicted mature protein 71 aa showed...
Summary Epsilon‐ Proteobacteria is increasingly recognized as an ecologically significant group of bacteria, particularly in deep‐sea hydrothermal environments. In this study, we studied the spatial distribution, diversity and physiological characteristics epsilon‐ various microbial habitats vicinity a vent occurring Iheya North field Mid‐Okinawa Trough, by using culture‐dependent ‐independent approaches. The were inside outside plume, annelid polychaete tubes. addition, deployed...
A novel mesophilic bacterium, strain GO25(T), was isolated from a nest of hydrothermal vent polychaetes, Paralvinella sp., at the Iheya North field in Mid-Okinawa Trough. Cells were motile short rods with single polar flagellum. Growth observed between 4 and 35 degrees C (optimum 30 C; 13-16 h doubling time) pH 5.4 8.6 6.1). The isolate facultatively anaerobic chemolithoautotroph capable growth using molecular hydrogen, elemental sulfur or thiosulfate as sole energy source, carbon dioxide...
Deep-sea vents are the light-independent, highly productive ecosystems driven primarily by chemolithoautotrophic microorganisms, in particular epsilon-Proteobacteria phylogenetically related to important pathogens. We analyzed genomes of two deep-sea vent strains, Sulfurovum sp. NBC37-1 and Nitratiruptor SB155-2, which provide insights not only into their unusual niche on seafloor, but also origins virulence pathogenic relatives, Helicobacter Campylobacter species. The...
ABSTRACT The carbon and energy metabolisms of a variety cultured chemolithoautotrophic Epsilonproteobacteria from deep-sea hydrothermal environments were characterized by both enzymatic genetic analyses. All the tested had all three key reductive tricarboxylic acid (rTCA) cycle activities—ATP-dependent citrate lyase, pyruvate:ferredoxin oxidoreductase, 2-oxoglutarate:ferredoxin oxidoreductase—while they no ribulose 1,5-bisphosphate carboxylase (RubisCO) activity, enzyme in Calvin-Benson...
Phase-separation and -segregation (boiling/distillation of subseafloor hydrothermal fluids) represent the primary mechanisms causing intra-field variations in vent fluid compositions. To determine whether this geochemical process affects formation microbial communities, we examined communities at three different sites located within a few tens meters one another. In addition to chimney structures, colonization devices capturing entrained by fluids were studied, using culture-dependent...
Geochemical characteristics of hydrothermal fluids in the Iheya North field, mid-Okinawa Trough, was investigated. Twelve-years observation reveals temporal variation vent fluid chemistry potentially controlled by temporally varying pattern phase-separation and -segregation, while constant Element/Cl ratios among periods chimneys indicate stable chemical composition source prior to undergoing phase-separation. The high K contents estimated are typical arc-backarc systems due reaction with...
A novel mesophilic, strictly hydrogen-oxidizing, sulfur-, nitrate- and thiosulfate-reducing bacterium, designated strain Monchim33(T), was isolated from a deep-sea hydrothermal vent chimney at the Central Indian Ridge. The non-motile, rod-shaped cells were Gram-stain-negative non-sporulating. Growth observed between 15 37 °C (optimum 33 °C; 3.2 h doubling time) pH 5.4 8.6 6.0). isolate anaerobic chemolithoautotroph capable of using molecular hydrogen as sole energy source carbon dioxide...
We report the successful cultivation and partial characterization of novel members epsilon-Proteobacteria, which have long been recognized solely as genetic signatures small subunit ribosomal RNA genes (rDNA) from a variety habitats occurring in deep-sea hydrothermal fields. A newly designed microhabitat designated 'in situ colonization system' was used for enrichment. Based on phylogenetic analysis rDNA isolates, most these represent first cultivated harboring previously uncultivated...
The structure and biosynthesis of poly- N -acetyllactosamine display a dramatic change during development oncogenesis. Poly- -acetyllactosamines are also modified by various carbohydrate residues, forming functional oligosaccharides such as sialyl Le x . Herein we describe the isolation expression cDNA encoding β-1,3- -acetylglucosaminyltransferase (iGnT), an enzyme that is essential for formation -acetyllactosamine. For this cloning, Burkitt lymphoma Namalwa KJM-1 cells were transfected...
Perfluorocarbon tracers (PFTs) are used during cruises of the Ocean Drilling Program (ODP) and Integrated (IODP) to measure sample contamination with drilling fluid. fluid is supplied a constant PFT concentration that can then be detected quantified in sediment basalt core samples. During IODP Expedition 301, we washing (2×) flaming effectively remove from exterior rocks. Near-complete removal allowed us demonstrate interior basalts was only minutely, if at all, contaminated We examined...
Two novel denitrifying bacteria, designated strains MI55-1 T and E9I37-1 , were isolated from deep-sea hydrothermal vent chimney structures at the Iheya North field in Mid-Okinawa Trough, Japan. Both isolates strict chemolithoautotrophs growing by respiratory nitrate reduction with H 2 forming N as a metabolic product. Oxygen (at low concentrations) could serve an alternative electron acceptor for growth of isolates. Growth strain was observed temperatures between 40 57 °C (optimum, 55 °C;...
The importance of crustal fluid chemical composition in driving the marine deep subseafloor biosphere was examined northeast Pacific ridge-flank sediments. At IODP Site U1301, sulfate from fluids diffuses into overlying sediments, forming a transition zone where meets situ-produced methane. Enhanced cell counts and metabolic activity suggest that stimulates microbial respiration, specifically anaerobic methane oxidation coupled to reduction. Cell are also elevated basement-near layers. Owing...
Two novel, thermophilic piezophiles, capable of chemolithoautotrophic growth, are successfully cultivated and isolated from a black smoker chimney at the TAG field (Mid Atlantic Ridge: MAR) by using piezophilic cultivation technique. Both strains (strains 106 108) represent dominant populations microbial communities in surface habitat. Strain represents typically thin, long spiral cells under growth condition but short bent non-piezophilic condition. It is strictly gammaproteobacterium...
A method is described for determination of lipid alpha glyceryl ethers in animal tissues. Purified tissue extracts are hydrolyzed and the soluble fraction hydrolysate fractionated on a silicic acid column. The ether oxidized with periodic formaldehyde formed determined colorimetrically. minor component all tissues examined except bone marrow. other than marrow range content from 0.20 to 1.35 micromoles per gram dry lipid-free 0.002 0.010 molar ratio phosphorus.