A5103281081- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- Plasma Diagnostics and Applications
- Fungal and yeast genetics research
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Adipose Tissue and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- Genetic Neurodegenerative Diseases
- RNA modifications and cancer
- Mass Spectrometry Techniques and Applications
- Cellular transport and secretion
- Protein Tyrosine Phosphatases
- Alzheimer's disease research and treatments
- Autophagy in Disease and Therapy
- Retinal Development and Disorders
- Cell death mechanisms and regulation
- Dust and Plasma Wave Phenomena
- Ionosphere and magnetosphere dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Endoplasmic Reticulum Stress and Disease
Université Toulouse III - Paul Sabatier
2012-2023
Centre National de la Recherche Scientifique
2012-2023
Centre de Recherches sur la Cognition Animale
2016-2023
Université de Toulouse
2009-2021
Centre de Biologie du Développement
2012-2015
Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération
1999-2010
Laboratoire de Biologie Moléculaire des Eucaryotes
2003
Laboratoire de Physique des Plasmas
2002
Institut de Pharmacologie et de Biologie Structurale
1996-1998
Office National d'Études et de Recherches Aérospatiales
1994-1995
OPA1 encodes a large GTPase related to dynamins, anchored the mitochondrial cristae inner membrane, facing intermembrane space. haplo-insufficiency is responsible for most common form of autosomal dominant optic atrophy (ADOA, MIM165500), neuropathy resulting from degeneration retinal ganglion cells and nerve atrophy. Here we show that down-regulation in HeLa using specific small interfering RNA (siRNA) leads fragmentation network concomitantly dissipation membrane potential drastic...
Mutations in the OPA1 gene are associated with autosomal dominant optic atrophy. encodes a dynamin‐related protein orthologous to Msp1 of Schizosaccharomyces pombe and Mgm1p Saccharomyces cerevisiae , both involved mitochondrial morphology genome maintenance. We present immuno‐fluorescence biochemical evidences showing that resides mitochondria where it is imported through its highly basic amino‐terminal extension. Proteolysis experiments indicate inter‐membrane space electron microscopy...
Using circular dichroism to probe the extent of DNA condensation in chromatin, we have demonstrated that a major nucleolar protein, nucleolin can decondense chromatin. By means various binding assays show has strong affinity for histone H1 and phosphorylated N-terminal domain, rich lengthy stretches acidic amino acids, is responsible this ionic interaction. Additional experiments clearly demonstrate unable act as nucleosome core assembly or disassembly factor hence little octamer. We propose...
Human OPA1 (optic atrophy type 1) is a dynamin-related protein of the mitochondrial IMS (intermembrane space) involved in membrane fusion and remodelling. Similarly to its yeast orthologue Mgm1p that exists two isoforms generated by serine protease Pcp1p/Rbd1p, various alternative splicing processing. In present paper, we focus on processing OPA1.We find mammalian cell types display similar pattern [two L-OPA1 (long OPA1) three S-OPA1 (short OPA1)] loss inner potential, but not inhibition...
Abstract The heterozygous R445H mutation in OPA1 was found five patients with optic atrophy and deafness. Audiometry suggested that the sensorineural deafness resulted from auditory neuropathy. Skin fibroblasts showed hyperfragmentation of mitochondrial network, decreased membrane potential, adenosine triphosphate synthesis defect. In addition, to be widely expressed sensory neural cochlear cells guinea pig. Thus, may related energy defects due a fragmented network. Ann Neurol 2005
Nucleolin is a ubiquitous multifunctional protein involved in preribosome assembly and associated with both nucleolar chromatin interphase organizer regions on metaphasic chromosomes mitosis. Extensive nucleolin phosphorylation by casein kinase (CKII) occurs serine growing cells. Here we report that while CKII achieved interphase, threonine during We provide evidence this type of vivo involves mammalian homolog the cell cycle control Cdc2 kinase. In vitro M-phase H1 from starfish oocytes...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhosphorylation of nucleolin by a nucleolar type NII protein kinaseMichelle Caizergues-Ferrer, Pascale Belenguer, Bruno Lapeyre, Francois Amalric, Michael O. Wallace, and Mark J. OlsonCite this: Biochemistry 1987, 26, 24, 7876–7883Publication Date (Print):December 1, 1987Publication History Published online1 May 2002Published inissue 1 December 1987https://pubs.acs.org/doi/10.1021/bi00398a051https://doi.org/10.1021/bi00398a051research-articleACS...
Abstract To characterize the molecular links between type‐1 autosomal dominant optic atrophy (ADOA) and OPA1 dysfunctions, effects of pathogenic alleles this dynamin on mitochondrial morphology apoptosis were analyzed, either in fibroblasts from affected individuals, or HeLa cells transfected with similar mutants. The missense substitutions GTPase domain (OPA1 G300E R290Q ) deletion effector Δ58 ). Fragmentation mitochondria increased cells. did not influence morphology, but sensitivity to...
Mitochondrial dynamics control the organelle's morphology, with fusion leading to formation of elongated tubules and fission isolated puncta, as well mitochondrial functions. Recent reports have shown that disruptions contribute neurodegenerative diseases. Mutations inner membrane GTPase OPA1 are responsible for type 1 dominant optic atrophy, by mechanisms not fully understood. We show here in rodent cortical primary neurons, downregulation protein leads fragmented mitochondria become less...
A comparison is made of the forces governing transport negatively charged particles electrostatically suspended in plasma a symmetric parallel-plate radiofrequency glow discharge with isothermal walls. The two driving symmetrically from centreline to sheath edges are ion drag force and plasma-induced thermophoresis due thermal gradient appearing gas heated by power dissipation. general expression as function ratio drift velocity obtained proper integration over energy distribution using...