A5100641821- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Immune cells in cancer
- Asthma and respiratory diseases
- Eosinophilic Esophagitis
- IL-33, ST2, and ILC Pathways
- Cytokine Signaling Pathways and Interactions
- Reproductive System and Pregnancy
- Toxin Mechanisms and Immunotoxins
Yonsei University
2019-2023
Seoul National University
2019
To this date, the criteria to distinguish peritoneal macrophages and dendritic cells (DCs) are not clear.Here we delineate subsets of myeloid mononuclear in mouse cavity.Considering phenotypical, functional, ontogenic features, divided into 5 subsets: large (LPMs), small (SPMs), DCs, 2 MHCII + CD11c CD115 subpopulations (i.e., CD14 -CD206 -and CD206 ).Among them, competent Ag presenting demonstrated with distinct functional characteristics, one being DCs other -cells.DCs able promote fully...
Dendritic cells (DCs) are key antigen-presenting that prime naive T and initiate adaptive immunity. Although the genetic deficiency transgenic overexpression of granulocyte macrophage-colony stimulating factor (GM-CSF) signaling were reported to influence homeostasis DCs, in vivo development DC subsets following injection GM-CSF has not been analyzed detail. Among treatment mice with different hematopoietic cytokines, only generates a distinct subset XCR1 - 33D1 DCs which make up majority...
Dendritic cells (DCs) in peripheral tissues may have a unique role to regulate innate and adaptive immune responses antigens that enter the tissues. Peritoneal cavity is body compartment surrounding various organs housing diverse cells. Here, we investigated specialized features of classical DC (cDC) subsets following intraperitoneal injection model antigen ovalbumin (OVA). cDC1s were superior cDC2s activating OVA-specific CD8 T cells, while both cDCs similar stimulating CD4 Each peritoneal...
<h3>Background:</h3> Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation with subsequent cartilage and bone erosion leading to joint destruction. The fibroblast-like synoviocytes (FLS) have a central role in pathogenesis vitro FLS invasiveness correlates articular damage RA patients. Spleen tyrosine kinase (Syk) intracellular protein the cells of tissues such as bone, cartilage, synovium, where it involved tumor necrosis factor α (TNF-α) receptor...
Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity. However, recent studies revealed that is a complex displaying variety of phenotypes and endotypes.We examined cellular in the mouse model allergic sensitized with different adjuvants. The aim our study to determine immunologic characteristics models induced ovalbumin (OVA) adjuvants.Mice were intraperitoneally admixture OVA various adjuvants such Alhydrogel (alum), papain, lipopolysaccharide...
Dendritic cells (DCs) are readily generated from the culture of mouse bone marrow (BM) treated with either granulocyte macrophage-colony stimulating factor (GM-CSF) or FMS-like tyrosine kinase 3 ligand (FLT3L). CD11c + MHCII hi routinely isolated those BM cultures and generally used as in vitro -generated DCs for a variety experiments therapies. Here, we examined GM-CSF FLT3L by staining monoclonal antibody 2A1 that is known to recognize mature activated DCs. Most within DC gate were (GM-BM...
Abstract Allergic asthma is a complex chronic disease which displays combined traits referred to as Th2 mediated eosinophilic allergy, ILC2 and Th17 cell neutrophilic allergy. In our current study of allergic model, the populations immune cells related well outside response were analyzed for ratio cytokines in IL-4, IL5, IL17A IFN-γ expressing CD4+ T utilizing BALB/c mice sensitized with ovalbumin different adjuvant such Alhydrogel (alum), papain, lipopolysaccharide (LPS), CpG. lung...
Abstract Granulocyte macrophage-colony stimulating factor (GM-CSF) is a key hematopoietic cytokine for the development of immune cells from progenitors in bone marrow (BM). However, role GM-CSF hematopoiesis outside BM has been less well understood. In recent report, we discovered that significant proliferation CD11bhi myeloid cells, including CD11chi dendritic (DCs) and CD11clo macrophages, were detected culture splenocytes with between 1 3 weeks. To further characterize this slow...
Abstract Dendritic cells (DCs) play pivotal roles in various immune responses by sampling and presenting antigens to naïve T inducing the subsequent T-cell activation or tolerance. In vitro culture systems have been widely used study DC biology. The of bone marrow (BM) with aids a hematopoietic cytokine, Fms-like tyrosine kinase 3 ligand (FLT3L), yields plasmacytoid DCs (pDCs) classical (cDCs), whereas BM presence granulocyte-macrophage colony-stimulating factor (GM-CSF) produces BM-derived...
Abstract Dendritic cells (DCs) play crucial parts in a variety of immune responses by capturing and presenting antigens to naïve T producing the subsequent T-cell activation or tolerance. In vitro culture systems DCs have been widely used. The bone marrow (BM) presence hematopoietic cytokine, Fms-like tyrosine kinase 3 ligand (FLT3L), produces plasmacytoid (pDCs) classical (cDCs), whereas BM with granulocyte-macrophage colony-stimulating factor (GM-CSF) generates macrophages considered...