Lipase from Rhizopus oryzae (ROL) exhibits remarkable sn-1,3 stereoselectivity and catalytic activity, but its poor thermostability limits applications in the production of 1,3-dioleoyl-2-palmitoyl glycerol (OPO, a high-quality substitute for human milk fat). In this work, semirational method was proposed to engineer activity 4M (ROL mutant our previous study). First, computer-aided design is performed using as template, N-glycosylation mutants are then recombinantly expressed screened...

This study aimed to analyze the impact of dental implant restoration on biomechanics temporomandibular joint (TMJ) in patients with posterior tooth loss. Ten healthy volunteers (Control group) and twenty loss (pre-operative Pre group, post-operative Post were recruited. Three-dimensional maxillofacial models maxilla, mandible, dentition articular discs reconstructed. The von Mises stress, contact tensile stress TMJ analyzed. Before restoration, stresses group considerably higher than those...

Anterior disc displacement (ADD) is one of the most prevalent temporomandibular disorders (TMD). It was widely recognized that occlusal factors could affect joint (TMJ). However, impacts ADD on biomechanical environment TMJ and occlusion are still unclear. This study aimed to describe effects masticatory system, including occlusion. The finite element model (FEM) constructed based medical images a healthy adult male. complete skull, muscles, TMJs, related ligaments were included. Three FEMs...

Currently, controversy persists over whether dental implant restoration exacerbates or alleviates temporomandibular disorders (TMDs). This study aimed to analyze the impact of on biomechanics joint (TMJ) in patients with posterior tooth loss. Ten healthy volunteers (Control group) and twenty loss (preoperative Pre group postoperative Post were recruited. Three-dimensional maxillofacial models maxilla, mandible, dentition, articular discs reconstructed. The von Mises, contact, tensile...

Sagittal split ramus osteotomy (SSRO) is the representative orthognathic surgery for patients with mandibular prognathism. It essential to understand biomechanical environment of temporomandibular joint (TMJ) analyse effects SSRO. This study aimed investigate influence SSRO on stress distributions in TMJs prognathism under symmetric occlusions. Thirteen and ten asymptomatic volunteers were recruited. Finite element models asymptomatic, preoperative, postoperative subjects established, based...

Purpose: This study aimed to analyze the changes of stress distributions in TMJs for pre- and postoperative patients with mandibular prognathism under unilateral occlusions, a frequent occlusion mastication. Methods: Pre- six-mouth cone-beam computed tomography images thirteen diagnosed were scanned used construct complete maxillofacial models, assigned as Pre Post group, respectively. Another ten asymptomatic individuals defined Control group. The inhomogeneous properties models. muscle...

Abstract The use of enzymes to catalyze Henry reaction has advantages mild conditions and low contamination, but enzyme activity promiscuous catalysis limits its application. Here, rational design was first performed identify the key amino acid residues in catalyzed by Lactococcal multidrug resistance Regulator (LmrR). Further, non-canonical acids were introduced into LmrR, successfully obtaining variants that enhanced catalytic LmrR. best variant, V15CNF, showed a 184% increase compared...

Abstract The purpose of this study is to investigate the biomechanical effect bimaxillary osteotomies on patients with mandibular retraction. Mandibular retraction, as a typical maxillofacial deformity, and has great adverse effects TMJ. Bimaxillary are widely used correct symptoms It necessary understand surgery temporomandibular disc (TMJ). Five retraction 10 asymptomatic subjects were involved in study. Finite element models preoperative, postoperative control group constructed based CT...

Abstract Murine collagen induced arthritis (CIA) is a widely used model of rheumatoid (RA). Identification CIA susceptibility genes will aid in the understanding RA pathogenesis and development therapeutic targets. This study aims to identify refine quantitative trait loci (QTL) controlling CIA. Major clinical traits were evaluated both (DBA/1×FVB/N) F2 advanced intercross line (AIL) mice; QTLs confirmed refined AIL. To search for candidate genes, we applied multiple approaches, including...

To analyze the effects of orthognathic surgery on stress distributions in temporomandibular joint (TMJ) patients with jaw deformity during unilateral molar clenching (UMC) by using three-dimensional (3D) finite element method.Nine (preoperative group, 26.1 ± 5.6 years old) and 9 asymptomatic subjects (control 22.0 6.0 were selected. Furthermore, also considered as postoperative group after undergoing surgery. Finite models for mandible, articular disc, maxilla developed through cone beam...

Abstract The purpose of this study was to investigate how sagittal split ramus osteotomy (SSRO) and Le Fort 1 affected the stress distribution temporomandibular joint (TMJ) during an anterior teeth bite using three-dimensional (3D) finite element (FE) method. Fourteen orthognathic surgery patients were examined with mandibular prognathism, facial asymmetry, retraction. They underwent in conjunction SSRO. In addition, ten asymptomatic subjects recruited as control group. 3D models mandible,...

To investigate the effect of bimaxillary surgery on stress distribution temporomandibular joint (TMJ) in patients with mandibular retrognathia under unilateral molar clenching (UMC). Five (preoperative group) and ten asymptomatic subjects (control were recruited. In addition, treated considered as postoperative group. The muscle forces corresponding to UMC applied. results showed that discal stresses group significantly greater than those preoperative control groups. Bimaxillary plus had a...

The pre-stress of the temporomandibular joint (TMJ) at intercuspal position (ICP) was often neglected, which would cause errors in finite element analysis. purpose this study to investigate effect on dynamic analysis TMJs. One healthy female adult recruited for medical imaging and motion data acquisition reference (RP) ICP clicking teeth. three-dimensional maxillofacial model including maxilla, mandible, articular cartilages, discs, discal attachments reconstructed. Motion from RP simulated...

Polymerization shrinkage is a major side effect of resin composite materials that affects the success and longevity caries restorations. This study was to analyze internal angle shape lining on residual stress dental restorations in Class II mesio-occlusal-distal (MOD) cavities through finite element analysis (FEA). A 3D reconstructed model human mandibular first molar created from micro-CT images, then tooth prepared as MOD cavity. models four regular corner shapes with different angles one...

OBJECTIVES To investigate the differences in temporomandibular joints (TMJs) between patients with anterior disc displacement reduction (ADDwR) and asymptomatic subjects by using 3D morphometric measurements. METHODS A total of 15 ADDwR 10 were enrolled. Then, models maxilla mandible reconstructed MIMICS 20.0. Nine morphologic parameters TMJs on both sides measured solid model. The analyzed left right each group. RESULTS horizontal coronal condylar angles ipsilateral side significantly...