A5074917831- Research on Leishmaniasis Studies
- Toxin Mechanisms and Immunotoxins
- Transgenic Plants and Applications
- vaccines and immunoinformatics approaches
- Trypanosoma species research and implications
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- T-cell and Retrovirus Studies
- Animal Virus Infections Studies
- Parasites and Host Interactions
- Immune Response and Inflammation
- Bacillus and Francisella bacterial research
- Synthesis and Biological Evaluation
- Macrophage Migration Inhibitory Factor
- Vaccine Coverage and Hesitancy
- RNA Interference and Gene Delivery
- COVID-19 Clinical Research Studies
Pasteur Hellenic Institute
2012-2024
Institut Pasteur
2014-2024
Subunit proteins provide a safe source of antigens for vaccine development especially intracellular infections which require the induction strong cellular immune responses. However, those are often limited by their low immunogenicity. In order to achieve effective responses, they should be encapsulated into stable antigen delivery system combined with an appropriate adjuvant. As such cationic liposomes efficient platform delivery. present study, we describe liposomal co-delivery and...
Leishmaniasis is a significant worldwide health problem for which no vaccine exists. Activation of CD4+ and CD8+ T cells crucial the generation protective immunity against parasite. Recent trend in design has been shifted to epitope-based vaccines that are more specific, safe, easy produce. In present study, four known antigenic Leishmania (L.) infantum proteins, CPA, histone H1, KMP-11 LeIF were analysed prediction binding epitopes H2d MHC class I II molecules, using online available...
Visceral leishmaniasis (VL), caused by Leishmania (L.) donovani and L. infantum protozoan parasites, can provoke overwhelming protracted epidemics, with high case–fatality rates. An effective vaccine against the disease must rely on generation of a strong long-lasting T cell immunity, mediated CD4+ TH1 CD8+ cells. Multi-epitope peptide-based development is manifesting as new era vaccination strategies infection. In present study, we designed chimeric peptides containing HLA-restricted...
Visceral leishmaniasis (VL) persists as a major public health problem, and since the existing chemotherapy is far from satisfactory, development of an effective vaccine emerges most appropriate strategy for confronting VL. The relies on selection antigen also right adjuvant and/or delivery vehicle. In present study, protective efficacy poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs), which were surface-modified with TNFα-mimicking eight-amino-acid peptide (p8) further...
Leishmaniasis is a vector-borne disease caused by an intracellular parasite of the genus Leishmania with different clinical manifestations that affect millions people worldwide, while visceral form may be fatal if left untreated. Since available chemotherapeutic agents are not satisfactory, vaccination emerges as most promising strategy for confronting leishmaniasis. In present study, reverse vaccinology approach was adopted to design pipeline starting from proteome analysis three species...
Background Through their increased potential to be engaged and processed by dendritic cells (DCs), nanovaccines consisting of Poly(D,L-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with both antigenic moieties adjuvants are attractive candidates for triggering specific defense mechanisms against intracellular pathogens. The aim the present study was evaluate immunogenicity prophylactic a rationally designed multi-epitope peptide Leishmania Cysteine Protease A (CPA160-189)...
Leishmaniases are complex vector-borne diseases caused by intracellular parasites of the genus Leishmania. The visceral form disease affects both humans and canids in tropical, subtropical, Mediterranean regions. One health approach has suggested that controlling zoonotic leishmaniasis (ZVL) could have an impact on reduction human incidence (VL). Despite fact a preventive vaccination help with elimination, effective vaccines able to elicit protective immune responses currently lacking. In...
Leishmania infantum is the etiologic agent of zoonotic visceral leishmaniasis (VL) in countries Mediterranean basin, where dogs are domestic reservoirs and represent important elements transmission disease. Since major focal areas human VL exhibit a high prevalence seropositive dogs, control canine could reduce infection rate humans. Efforts toward this have focused on improvement diagnostic tools, as well vaccine development. The identification parasite antigens including suitable...
Since the initiation of COVID-19 pandemic, there has been a need for development diagnostic methods to determine factors implicated in mounting an immune response against virus. The most promising indicator suggested be neutralizing antibodies (nAbs), which mainly block interaction between Spike protein (S) SARS-CoV-2 and host entry receptor ACE2. In this study, we aimed develop optimize conditions competitive ELISA measure serum titer, using recombinant trimeric modified have six additional...
Visceral leishmaniasis (VL) caused by Leishmania donovani and L. infantum is a potentially fatal disease. To date there are no registered vaccines for disease prevention despite the fact that several in preclinical development. Thus, new strategies needed to improve vaccine efficacy based on better understanding of mechanisms mediating protective immunity host immune responses subversion immunopathogenic components Leishmania. We found mice vaccinated with CPA162-189-loaded p8-PLGA...
Leishmania parasites are capable of effectively invading dendritic cells (DCs), a cell population orchestrating immune responses against several diseases, including leishmaniasis, by bridging innate and adaptive immunity. on the other hand has evolved various mechanisms to subvert DCs activation establish infection. Thus, transcriptional profile derived from bone marrow (BMDCs) that have been infected with infantum parasite or exposed chemically inactivated was investigated via RNA...
Abstract Background Based on the fact that coronavirus disease 2019 (COVID-19) is still spreading despite worldwide vaccine administration, there an imperative need to understand underlying mechanisms of vaccine-induced interindividual immune response variations. Methods We compared humoral and cellular responses in 127 individuals vaccinated with either BNT162b2, mRNA-1273, or ChAdOx1-nCoV-19 vaccine. Results Both mRNA vaccines induced faster stronger as assessed by high spike- RBD-specific...
For many infectious diseases T cells are an important part of naturally acquired protective immune responses, and inducing these by vaccination has been the aim much research.Here, we describe a protocol for analysis vaccine-induced antigen-specific responses.For this purpose, whole spleens obtained from vaccinated BALB/c mice were ex vivo stimulated with antigen incorporated in vaccine.Evaluation characterization adaptive cell responses was performed assaying spleen proliferation through...
Control of the intracellular parasite Leishmania (L.) requires activation strong type 1 cellular immune responses. Towards this goal, in present study, a multiepitope chimeric protein named LiChimera was encapsulated into cationic liposomes and its protective efficacy against experimental visceral leishmaniasis investigated. Liposomal conferred significant protection L. infantum as evidenced by significantly reduced loads spleen liver. Protection detected Lipo:LiChimera-immunized mice...
Dendritic cells (DCs) play a pivotal role in promoting resistance to leishmaniasis, both by activating CD4 + T and endorsing their differentiation into Th1 producing interleukin (IL)-12. High level of IL-12 production, decisive component the DC maturation, requires not only microbial stimuli but also strong CD40-CD40L interactions. Until now, mechanisms which Leishmania (L.) infantum parasites affect functional maturation consequently cell polarization are fully understood. In present study,...
Event Abstract Back to In silico prediction of promiscuous Leishmania infantum KMP-11, H1, LeIF, CPA, CPB peptides and experimental validation eliciting CD4+ CD8+ T-cell specific responses Maria Agallou1, Olga Koutsoni1, Eleni Dotsika1 Evdokia Karagouni1* 1 Hellenic Pasteur Institute, Greece Previous studies on leishmaniasis have demonstrated that both IFNγ-producing T lymphocytes are significant players in protection mechanisms against visceral disease (Alexander Brombacher, 2012; Stager...