A5043404598- Advanced Fluorescence Microscopy Techniques
- Mitochondrial Function and Pathology
- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- Genomics and Chromatin Dynamics
- Elasticity and Material Modeling
- Cardiovascular Function and Risk Factors
- ATP Synthase and ATPases Research
- Heat shock proteins research
- Photoacoustic and Ultrasonic Imaging
- Advanced Neuroimaging Techniques and Applications
- Cardiomyopathy and Myosin Studies
- Adipose Tissue and Metabolism
- Advanced MRI Techniques and Applications
- Digital Holography and Microscopy
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Spaceflight effects on biology
- Nanoplatforms for cancer theranostics
- Spectroscopy Techniques in Biomedical and Chemical Research
- thermodynamics and calorimetric analyses
- Skin and Cellular Biology Research
- Advanced X-ray Imaging Techniques
- Optical Imaging and Spectroscopy Techniques
- Morphological variations and asymmetry
Centre National de la Recherche Scientifique
2013-2023
Université Grenoble Alpes
2008-2023
VetAgro Sup
2004-2023
Institut polytechnique de Grenoble
2021-2023
Centre Hospitalier Universitaire de Grenoble
2000-2023
Translational Innovation in Medicine and Complexity
2012-2022
Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement
2021
Canadian Nautical Research Society
2018
Inserm
1997-2015
Université Joseph Fourier
2004-2015
We synthesized a generation of water-soluble, atomically precise gold nanoclusters (Au NCs) with anisotropic surface containing short dithiol pegylated chain (AuMHA/TDT). The AuMHA/TDT exhibit high brightness (QY ∼ 6%) in the shortwave infrared (SWIR) spectrum detection above 1250 nm. Furthermore, they show an extended half-life blood (t1/2ß = 19.54 ± 0.05 h) and very weak accumulation organs. also developed non-invasive, whole-body vascular imaging system SWIR window high-resolution,...
The Pseudomonas aeruginosa cystic fibrosis isolate CHA induces type III secretion system‐dependent but ExoU‐independent oncosis of neutrophils and macrophages. Time‐lapse microscopy the infection process revealed rapid accumulation motile bacteria around infected cells undergoing oncosis, a phenomenon we termed pack swarming. Characterization non‐chemotactic cheZ mutant showed that swarming is bacterial chemotactic response to A non‐cytotoxic mutant, lacking III‐secreted proteins PcrV, PopB...
Mitochondria play a critical role in myocardial cold ischemia-reperfusion (CIR) and induction of apoptosis. The nature extent mitochondrial defects cytochrome c (Cyt c) release were determined by high-resolution respirometry permeabilized fibers. CIR rat heart transplant model resulted variable contractile performance, correlating with the decline ADP-stimulated respiration. Respiration succinate or N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride (substrates for complexes II IV) was...
Heat shock factor 1 (HSF1) is essential for the stress-induced expression of heat genes. On exposure to shock, HSF1 localizes within seconds discrete nuclear granules. recovery from rapidly dissipates these stress granules a diffuse nucleoplasmic distribution, typical unstressed cells. Subsequent reexposure results in rapid relocalization same with identical kinetics. Although appearance corresponds hyperphosphorylated, trimeric DNA-binding state and correlates temporally inducible...
We have recently demonstrated that heterochromatin HP1 proteins are aberrantly distributed in lymphocytes of patients with immunodeficiency, centromeric instability and facial dysmorphy (ICF) syndrome. The three accumulate one giant body over the 1qh 16qh juxtacentromeric heterochromatins, which hypomethylated ICF. presence PML (promyelocytic leukaemia) protein within this suggests it to be a nuclear (PML-NB). structural integrity PML-NBs is major importance for normal cell functioning....
Abstract Vessel size imaging (VSI) for brain tumor characterization was evaluated and the vessel index measured by MRI (VSI ) correlated with VSI obtained histology histo ). Blood volume (BV) maps were on 12 rats simultaneous measurements of R 2 * , before after injection a macromolecular contrast agent, AMI‐227. Immunostaining collagen IV in vessels performed. An expression derived evaluating from stereologic data On BV images large‐size tumors (n = 9), three regions could be distinguished...
ABSTRACT The distribution of secondary myotubes and undifferentiated mononucleated cells (presumed to be myoblasts) within foetal IVth lumbrical muscles the rat was analyzed with serial section electron microscopy. In all myotube clusters for which innervation zone located, every overlapped endplate region primary myotube. No were ever demonstrated occur at a distance from zone. This indicates that new begin form only in muscle. Some young made direct contact nerve terminal, but we cannot...
In this study we analyse the formation and dynamics of specific actin-rich structures called podosomes. Podosomes are very dynamic punctual adhesion sites tightly linked to actin cytoskeleton. Mechanical properties substrates emerging as important physical modulators anchorage-dependent processes involved in cellular response. We investigate influence substrate flexibility on used mouse NIH-3T3 fibroblasts, transfected with GFP-actin cultured polyacrylamide collagen-coated varying stiffness....
The extracellular matrix protein “elastin” is the major component of elastic fibers present in arterial wall. Physiological degradation fibers, enhanced vascular pathologies, leads to presence circulating elastin peptides (EP). EP have been demonstrated influence cell migration and proliferation. also induce, at pathophysiological concentrations (and not below), an endothelium- NO- dependent vasorelaxation mediated by 67-kDa subunit elastin-laminin receptor. Here, using techniques...
They present results for lens-free microscopy the imaging of dense cell culture. With this aim, they use a multiwavelength LED illumination with well separated wavelengths, together implementation an appropriate holographic reconstruction algorithm. This allows fast and efficient phase image densely packed cells (up to 700 cells/mm2 ) over large field view 29.4 mm2 . Combined compactness system which fits altogether inside incubator, becomes unique tool monitor cultures several days. The...
The sperm nucleus has a unique chromatin structure where the DNA is highly condensed and associated with specific proteins, protamines. It nondividing cell which also transcriptionally inactive. After fusion an oocyte, undergoes decondensation and, in same time, starts replication transcription. been suggested that somatic chromosomes during interphase are organized territories display type cycle distribution. purpose of this work was to investigate whether would have distribution nucleus,...
ABSTRACT Desmoplakin is an intracellular component of desmosomes which plays a role in the anchorage intermediate filaments to these structures. We report here that, despite absence desmosomes, cultured endothelial cells from human umbilical vein express desmoplakin I and II both at mRNA protein level. I/II are found only detergent insoluble fraction suggesting that most linked cytoskeleton. could be detected by western blot long confluent even if levels unchanged cell confluency. This...