A5078086057- Redox biology and oxidative stress
- Photosynthetic Processes and Mechanisms
- Metal-Catalyzed Oxygenation Mechanisms
- Heat shock proteins research
- Mitochondrial Function and Pathology
- Protein Structure and Dynamics
- Lipid metabolism and biosynthesis
- Virus-based gene therapy research
- bioluminescence and chemiluminescence research
- Viral Infectious Diseases and Gene Expression in Insects
Universidad Publica de Navarra
2011-2013
Gobierno de Navarra
2011-2013
Agrobiotechnology Institute
2011-2013
Consejo Superior de Investigaciones Científicas
2013
Universidad de Navarra
2012
Zero to Three
2011
In addition to the linear electron flow, a cyclic flow (CEF) around photosystem I occurs in chloroplasts. CEF, electrons back from donor site of plastoquinone pool via two main routes: one that involves Proton Gradient Regulation5 (PGR5)/PGRL1 complex (PGR) and is dependent NADH dehydrogenase-like complex. While importance CEF photosynthesis photoprotection has been clearly established, little known about its regulation. We worked on assumption redox regulation surveyed putative role...
Summary Starch, the most abundant storage carbohydrate in plants, has been a major feedstock for first‐generation biofuels. Growing fuel demands require, however, that starch yields of energy crops be improved. Leaf is synthesised during day and degraded at night to power nonphotosynthetic metabolism. Redox regulation associated with coordination enzymes involved metabolism, but neither signals nor mechanisms regulate this metabolism are entirely clear. In work, thioredoxin ( T rx) f m genes...
Thioredoxins (Trxs) are ubiquitous disulphide reductases that play important roles in the redox regulation of many cellular processes. However, some redox-independent functions, such as chaperone activity, have also been attributed to Trxs recent years. The focus our study is on putative function well-described plastid f and m. To end, cDNA both Trxs, designated NtTrxf NtTrxm, was isolated from Nicotiana tabacum plants. It found bacterially expressed tobacco Trx m, addition their reductase...
Plants display a remarkable diversity of thioredoxins (Trxs), reductases controlling the thiol redox status proteins. The physiological function many them remains elusive, particularly for plastidial Trxs f and m, which are presumed based on biochemical data to regulate photosynthetic reactions carbon metabolism. Recent reports revealed that m participate in vivo control starch metabolism cyclic electron transfer around photosystem I, respectively. To further delineate their planta function,...
Summary Thioredoxins (Trxs) are small ubiquitous disulphide proteins widely known to enhance expression and solubility of recombinant in microbial systems. Given the common evolutionary heritage chloroplasts bacteria, we attempted analyse whether plastid Trxs could also act as modulators protein transgenic chloroplasts. For that purpose, two tobacco (m f) with different phylogenetic origins were assessed. Using transformation, assayed strategies: fusion co‐expression human serum albumin...
Human cardiotrophin 1 (CT1), a cytokine with excellent therapeutic potential, was previously expressed in tobacco chloroplasts. However, the growth conditions required to reach highest expression levels resulted an impairment of its bioactivity. In present study, we have examined new strategies modulate this recombinant protein chloroplasts so as enhance production and particular, assessed effect both fusion co-expression Trx m CT1 on functional by using plastid transformation. Our data...