A5035963776- Aquaculture disease management and microbiota
- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Biochemical and Molecular Research
- Animal Virus Infections Studies
- Meat and Animal Product Quality
- Cancer therapeutics and mechanisms
- Infection Control in Healthcare
- Microbial infections and disease research
- Mycobacterium research and diagnosis
- Climate Change and Health Impacts
- Infection Control and Ventilation
- Genomics and Phylogenetic Studies
- Parasite Biology and Host Interactions
- Identification and Quantification in Food
- Marine Bivalve and Aquaculture Studies
- Evolution and Genetic Dynamics
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Marine animal studies overview
- Fish Biology and Ecology Studies
- Aquatic life and conservation
- Drug Transport and Resistance Mechanisms
- Invertebrate Immune Response Mechanisms
- Myxozoan Parasites in Aquatic Species
Rutgers, The State University of New Jersey
2019-2024
Rutgers New Jersey Medical School
2019-2022
Rutgers Health
2020
United States Geological Survey
2009-2012
Western Fisheries Research Center
2010-2012
NOAA National Marine Fisheries Service Northwest Fisheries Science Center
2011
Abstract Little is known about the physiology of latent Mycobacterium tuberculosis infection. We studied mutational rates 24 index (TB) cases and their latently infected household contacts who developed active TB up to 5.25 years later, as an indication bacterial physiological state possible generation times during infection in humans. Here we report that rate new mutations M. genome decline dramatically after two (two-sided p < 0.001, assuming 18 h time equal log phase , with latency...
Abstract H37Rv is the most widely used Mycobacterium tuberculosis strain, and its genome globally as M. reference sequence. Here, we present Bact-Builder, a pipeline that uses consensus building to generate complete accurate bacterial sequences apply it three independently cultured sequenced aliquots of single laboratory stock. Two 4,417,942 base-pair long assemblies are 100% identical, with third differing by nucleotide. Compared existing reference, new sequence contains ~6.4 kb additional...
The triazine antitubercular JSF-2019 was of interest due to its in vitro efficacy and the nitro group shared with clinically relevant delamanid pretomanid. undergoes activation requiring F420H2 one or more nitroreductases addition Ddn. An intrabacterial drug metabolism (IBDM) platform leveraged demonstrate system kinetics, evidencing formation NO⋅ a des-nitro metabolite. Structure-activity relationship studies focused on improving solubility mouse pharmacokinetic profile culminated JSF-2513,...
Abstract Laboratory challenges using specific-pathogen-free Pacific herring Clupea pallasii from three distinct populations indicated that stock origin had no effect on susceptibility to viral hemorrhagic septicemia (VHS). All of the were highly susceptible disease upon initial exposure, with significantly greater cumulative mortalities occurring in exposed treatment groups (56.3–64.3%) than unexposed control (0.8–9.0%). Interstock differences mortality not significant. The virus loads...
Abstract Groups of specific-pathogen-free Pacific herring Clupea pallasii were highly susceptible to infection by viral hemorrhagic septicemia virus (VHSV); however, the level mortality was influenced diet during 40–71 d before, during, and after first exposure virus. Cumulative highest among maintained on an experimental soy-based pellet, intermediate those a commercially available fish-meal-based lowest second pellet containing β-glucans. Additionally, feed demonstrated less growth than...
Abstract Multidrug-resistant tuberculosis (MDR-TB), defined as resistance to the first-line drugs isoniazid and rifampin, is a growing source of global mortality threatens control disease. The diarylquinoline bedaquiline has recently emerged highly efficacious drug against MDR-TB kills Mycobacterium by inhibiting mycobacterial ATP synthase. However, mechanisms underlying bedaquiline’s efficacy remain unknown. Here we investigate hyper-susceptibility in drug-resistant using systems biology...
Abstract The plasma of Pacific herring Clupea pallasii that survived laboratory-induced viral hemorrhagic septicemia (VHS) epizootics contained humoral substances that, when injected into naïve animals, conferred passive immunity against the disease. Among groups exposed to virus (VHSV), injection donor from VHS survivors resulted in significantly greater survival (50%) and lower tissue titers (1.5 × 105 plaque-forming units [PFU]/g) than VHSV-naïve donors (6% survival; 3.7 106 PFU/g)....
Viral haemorrhagic septicaemia virus, Genogroup IVa (VHSV), was highly infectious to Pacific herring, Clupea pallasii (Valenciennes), even at exposure doses occurring below the threshold of sensitivity for a standard viral plaque assay; however, further progression disease population-level epizootic required amplification and effective fish-to-fish transmission. Among groups herring injected with VHSV, prevalence infection dose-dependent, ranging from 100%, 75% 38% after 19, 0.7 0.07...
Abstract Epizootics of viral erythrocytic necrosis (VEN) occurred among juvenile Pacific herring Clupea pallasii in Skagit Bay, Puget Sound, Washington, during 2005–2007 and were characterized by high prevalences intensities cytoplasmic inclusion bodies within circulating erythrocytes. The prevalence VEN peaked at 67% the first epizootic October 2005 waned to 0% August 2006. A second throughout summer 2007; this was disease initiation perpetuation age-1, 2006 year-class, followed involvement...
Abstract Viral erythrocytic necrosis (VEN) is a condition that affects marine and anadromous fish species, including herrings salmonids, in the Atlantic Pacific oceans. Infection frequently associated with severe anemia causes episodic mortality among wild hatchery when accompanied by additional stressors; VEN can be presumptively diagnosed (1) light microscopic identification of single characteristic—a round, magenta-colored, 0.8-μm-diameter inclusion body (IB) within cytoplasm erythrocytes...
Abstract Procedures for a viral replication in excised fin tissue (VREFT) assay were adapted to Pacific herring, Clupea pallasii, and optimized both reduce processing time provide the greatest resolution between naïve herring those previously exposed haemorrhagic septicaemia virus (VHSV), Genogroup IVa. The procedures included removal of left pectoral from euthanized fish, inoculation with >10 5 plaque‐forming units (PFU) mL −1 VHSV 1 h, rinsing fresh medium six times remove unadsorbed...
The effects of temperature and infection by Ichthyophonus were examined in juvenile Pacific herring ( Clupea pallasii ) maintained under simulated overwinter fasting conditions. In addition to defining parameters for a bioenergetics model (discussed Vollenweider et al. this issue), these experiments provided new insights into factors influencing the infectivity virulence parasite . groups fish with established disease, variation had little effect on disease outcome. mortality outpaced that...
Summary Background The COVID-19 pandemic has caused a severe shortage of personal protective equipment (PPE), especially N95 respirators. Efficient, effective and economically feasible methods for large-scale PPE decontamination are urgently needed. Aims (1) to develop protocols effectively decontaminating using vaporized hydrogen peroxide (VHP); (2) novel approaches that decrease set up take down time while also increasing capacity (3) test efficiency respirators heavily contaminated by...
ABSTRACT Methionine biosynthetic pathway, essential for the growth of Mycobacterium tuberculosis ( Mtb ) in host, represents an attractive target development novel anti-tuberculars. Here, we have biochemically characterized homoserine acetyl transferase (HSAT viz. MetA) , which catalyses first committed step methionine and S-adenosylmethionine (SAM) biosynthesis. High-throughput screening a 2300 compound library resulted identification thiram, anti-fungal organosulfur compound, as most...
SUMMARY Background COVID-19 has stretched the ability of many institutions to supply needed personal protective equipment, especially N95 respirators. decontamination and reuse programs provide one potential solution this problem. Unfortunately, a comprehensive evaluation effects on integrity various models using quantitative fit test (QTFT) approach is lacking. Aims 1) To investigate up eight rounds vaporized H 2 O (VHP) respirators currently in use hospital setting. 2) examine if worn by...
Methionine biosynthetic pathway, essential for the growth of Mycobacterium tuberculosis (Mtb) in host, represents an attractive target development novel anti-tuberculars. Here, we have biochemically characterized homoserine acetyl transferase (HSAT viz. MetA) Mtb, which catalyses first committed step methionine and S-adenosylmethionine (SAM) biosynthesis. High-throughput screening a 2300 compound library resulted identification thiram, anti-fungal organosulfur compound, as most potent MetA...
ABSTRACT Rapid emergence of drug resistance in Mycobacterium tuberculosis (Mtb) is one the most significant healthcare challenges our time. The cause multifactorial, with long course anti-tubercular therapy required to treat (TB) constituting a major contributing factor. Introduction pyrazinamide (PZA) resulted shortening TB treatment from twelve six months and consequently played critical role curbing that developed over therapy. Nevertheless, because PZA prodrug activated by nonessential...
Abstract H37Rv is the most widely used M. tuberculosis strain. Its genome globally as reference sequence. We developed Bact-Builder, a pipeline that leverages consensus building to generate complete and highly accurate gap-closed bacterial genomes applied it three independently sequenced cultures of parental laboratory stock. Two 4,417,942 base-pair long assemblies were 100% identical, with third differing by single nucleotide. Compared existing reference, new sequence contained...