A5042516293- Platelet Disorders and Treatments
- Cell Adhesion Molecules Research
- Blood properties and coagulation
- Angiogenesis and VEGF in Cancer
- Erythrocyte Function and Pathophysiology
- Atherosclerosis and Cardiovascular Diseases
- Hemoglobin structure and function
- Single-cell and spatial transcriptomics
- Venous Thromboembolism Diagnosis and Management
- Nanoplatforms for cancer theranostics
- Cellular Mechanics and Interactions
- S100 Proteins and Annexins
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Spaceflight effects on biology
- Complement system in diseases
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Cancer-related gene regulation
- Advanced Electron Microscopy Techniques and Applications
Max Planck Institute for Molecular Biomedicine
2020-2024
The extravasation of leukocytes is a critical step during inflammation that requires the localized opening endothelial barrier. This process initiated by close interaction with various adhesion molecules such as ICAM-1 on surface cells. Here we reveal mechanical forces generated leukocyte-induced clustering synergize fluid shear stress exerted flowing blood to increase plasma membrane tension and activate mechanosensitive cation channel PIEZO1. leads increases in [Ca2+]i activation...
C-terminal Src kinase (Csk) targets family kinases (SFKs) and thereby inactivates them. We have previously shown that Csk binds to phosphorylated tyrosine 685 of VE-cadherin, an adhesion molecule major importance for the regulation endothelial junctions. This residue is SFK target, its mutation (VE-cadherin-Y685F) inhibits induction vascular permeability in various inflammation models. Nevertheless, surprisingly, it increases leukocyte extravasation. Here, we investigated whether involved...
Circulating leukocytes enter tissue either through endothelial junctions (paracellular) or via a pore the body of cells (transcellular). We have previously shown that genetically replacing VE-cadherin with VE-cadherin-α-catenin (VEC-αC) fusion construct-which binds constitutively to actin-obstructs junctions, and blocks leukocyte extravasation in lung, skin postcapillary venules cremaster muscle. However, neutrophil recruitment into inflamed peritoneal cavity was unimpaired. Investigating...
Intravital microscopy has emerged as a powerful imaging tool, which allows the visualization and precise understanding of rapid physiological processes at sites inflammation in vivo, such vascular permeability leukocyte migration. Leukocyte interactions with endothelium can be characterized living organism murine cremaster muscle. Here, we present technique using an Airy Beam Light Sheet microscope that significant advantages over our previously used confocal systems. In comparison, light...
The nanometer spatial resolution of electron microscopy imaging remains an advantage over light microscopy, but the restricted field view that can be inspected and inability to visualize dynamic cellular events are definitely drawbacks standard transmission (TEM). Several methods have been developed overcome these limitations, mainly by correlating microscopical image microscope with correlative (CLEM) techniques. Since there is more than one method obtain region interest (ROI), workflow...
Abstract The extravasation of leukocytes is a critical step during inflammation which requires the localized opening endothelial barrier. This process initiated by close interaction with various adhesion molecules such as intercellular molecule-1 (ICAM-1) on surface cells. It still unclear how these initial processes induce downstream signaling events resulting in inter-endothelial junctions to allow leukocyte diapedesis. Here we show that mechanical forces induced leukocyte-induced...