A5068160677- Genetic and Kidney Cyst Diseases
- Genetics, Aging, and Longevity in Model Organisms
- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
- Congenital heart defects research
- Biomedical Research and Pathophysiology
- FOXO transcription factor regulation
- Circadian rhythm and melatonin
- Genetic Syndromes and Imprinting
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Renal and related cancers
- Epigenetics and DNA Methylation
- Photosynthetic Processes and Mechanisms
- Spaceflight effects on biology
- Algal biology and biofuel production
Worcester Polytechnic Institute
2020-2023
Brandeis University
2013-2021
Case Western Reserve University
2008-2016
The primary cilium is nucleated by the mother centriole-derived basal body (BB) via as yet poorly characterized mechanisms. BBs have been reported to degenerate following ciliogenesis in C. elegans embryo, although neither BB architecture nor early steps described this organism. In a previous study (Doroquez et al., 2014), we three-dimensional morphologies of sensory neuron cilia adult hermaphrodites at high resolution. Here, use serial section electron microscopy and tomography staged...
Transcription factors are essential for regulating neuronal microtubules (MTs) during development and after axon damage. In this paper, we identify a novel function Drosophila melanogaster FoxO in limiting MT stability at the neuromuscular junction (NMJ). foxO loss-of-function NMJs displayed augmented stability. contrast, motor overexpression of wild-type moderately destabilized MTs, whereas constitutively nuclear severely MTs. Thus, negatively regulates synaptic family members...
FoxO proteins are evolutionarily conserved regulators of neuronal structure and function, yet the neuron-specific pathways within which they act poorly understood. To elucidate function in Drosophila melanogaster, we first screened for FoxO's upstream downstream effectors. On side, present genetic molecular pathway analyses indicating that Toll-6 receptor, Toll/interleukin-1 receptor domain adaptor dSARM, a linear pathway. find Toll-6-FoxO signaling represses mitotic kinesin Pavarotti/MKLP1...
The structure and function of primary cilia are critically dependent on intracellular trafficking pathways that transport ciliary membrane protein components. mechanisms by which these regulated not fully characterized. Here we identify the transmembrane OSTA-1 as a new regulator shape morphology composition sensory in C. elegans. osta-1 encodes an organic solute transporter alpha-like protein, mammalian homologs have been implicated transport, although role regulating has previously...
The structure and function of primary cilia are critically dependent on intracellular trafficking pathways that transport ciliary membrane protein components. mechanisms by which these regulated not fully characterized. Here we identify the transmembrane OSTA-1 as a new regulator shape morphology composition sensory in C. elegans. osta-1 encodes an organic solute transporter alpha-like protein, mammalian homologs have been implicated transport, although role regulating has previously...
Heterotrimeric G (αβγ) proteins are canonical transducers of G-protein-coupled receptor (GPCR) signaling and play critical roles in communication between cells their environment. Many GPCRs heterotrimeric localize to primary cilia modulate morphology via mechanisms that not well understood. Here, we show RIC-8, a cytosolic guanine nucleotide exchange factor (GEF) chaperone for Gα protein subunits, shapes membrane subset
Heterotrimeric G (αβγ) proteins are canonical transducers of G-protein-coupled receptor (GPCR) signaling and play critical roles in communication between cells their environment. Many GPCRs heterotrimeric localize to primary cilia modulate morphology via mechanisms that not well understood. Here, we show RIC-8, a cytosolic guanine nucleotide exchange factor (GEF) chaperone for Gα protein subunits, shapes membrane subset Caenorhabditis elegans sensory neurons. Consistent with its role...
ABSTRACT Primary cilia are located at the dendritic tips of sensory neurons and house molecular machinery necessary for detection transduction stimuli. The mechanisms that coordinate dendrite extension with cilium position during neuron development not well understood. Here, we show GRDN-1, Caenorhabditis elegans ortholog highly conserved scaffold signaling protein Girdin/GIV, regulates both in postembryonic AQR PQR gas-sensing neurons. Mutations grdn-1 disrupt outgrowth mislocalize to soma...