A5087900010- Neurobiology and Insect Physiology Research
- Invertebrate Immune Response Mechanisms
- Cellular transport and secretion
- Insect Resistance and Genetics
- Silk-based biomaterials and applications
- Genetics, Bioinformatics, and Biomedical Research
- Antimicrobial Peptides and Activities
- Various Chemistry Research Topics
- Adhesion, Friction, and Surface Interactions
- Skin and Cellular Biology Research
- Plant Stress Responses and Tolerance
- Biomedical and Engineering Education
- Insect symbiosis and bacterial influences
Comenius University Bratislava
2014-2024
Institute of Experimental Endocrinology of the Slovak Academy of Sciences
2014-2023
Slovak Academy of Sciences
2014-2023
In contrast to the well defined mechanism of merocrine exocytosis, apocrine secretion, which was first described over 180 years ago, remains relatively uncharacterized. We identified secretory activity in late prepupal salivary glands Drosophila melanogaster just prior execution programmed cell death (PCD). The excellent genetic tools available provide an opportunity dissect for time molecular and mechanistic aspects this process. A prerequisite such analysis is have pivotal...
Abstract One of the major functions larval salivary glands (SGs) many Drosophila species is to produce a massive secretion during puparium formation. This so-called proteinaceous glue exocytosed into centrally located lumen, and subsequently expectorated, serving as an adhesive attach puparial case solid substrate metamorphosis. Although this was first described almost 70 years ago, detailed description morphology mechanical properties largely missing. Its main known physical property that...
A central function of the Drosophila salivary glands (SGs), historically known for their polytene chromosomes, is to produce and then release during pupariation secretory glue used affix a newly formed puparium substrate. This essential event in life history regulated by steroid hormone ecdysone late-larval period. Ecdysone triggers cascade sequential gene activation that leads secretion initiates developmentally-regulated programmed cell death (PCD) larval glands, which culminates 16 h...
Apocrine secretion is a transport and secretory mechanism that remains only partially characterized, even though it evolutionarily conserved among all metazoans, including humans. The excellent genetic model organism Drosophila melanogaster holds promise for elucidating the molecular mechanisms regulating this fundamental metazoan process. Two prerequisites such investigations are to clearly define an experimental system investigate apocrine understand functional contexts in which arose...
In the recent past, we demonstrated that a great deal is going on in salivary glands of Drosophila interval after they release their glycoprotein-rich secretory glue during pupariation. The early-to-mid prepupal undergo extensive endocytosis with widespread vacuolation cytoplasm followed by massive apocrine secretion. Here, describe additional novel properties these endosomes. use vital pH-sensitive probes provided confirmatory evidence endosomes have acidic contents and there are two types...
The Drosophila salivary glands (SGs) were well known for the puffing patterns of their polytene chromosomes and so became a tissue choice to study sequential gene activation by steroid hormone ecdysone. One well-documented function these is produce secretory glue, which released during pupariation fix freshly formed puparia substrate. Over past two decades SGs have been used address specific aspects developmentally-regulated programmed cell death (PCD) as it was thought that they are doomed...
At the onset of Drosophila metamorphosis steroid hormone ecdysone induces a process leading to rapid degeneration larval salivary glands (SGs). Ecdysone acts through receptor heterodimer, which activates primary response genes. In particular these genes include Broad-Complex (BR-C) gene encoding set BTB/POZ-transcription factors, among Z1 isoform is critical for SG cell death. The timing disappearance depends upon p127l(2)gl, cytoskeletal tumor suppressor that interacts with nonmuscle myosin...
As future scientists, university students need to learn how avoid making errors in their own manuscripts, as well identify flaws papers published by peers. Here we describe a novel approach on promote students' ability critically evaluate scientific articles. The exercise is based instructing teams of write intentionally flawed manuscripts describing the results simple experiments. are supervised instructors advising during manuscript writing, choosing ‘appropriate’ errors, monitoring...
Apocrine secretion is a recently discovered widespread non-canonical and non-vesicular secretory mechanism whose regulation purpose only partly defined. Here, we demonstrate that apocrine in the prepupal salivary glands (SGs) of Drosophila provides sole source immune-competent defense-response proteins to exuvial fluid lies between metamorphosing pupae its pupal case. Genetic ablation delivery from SGs decreases survival microbial challenges, isolated has strong antimicrobial effects...