A5045630896- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Cancer, Hypoxia, and Metabolism
- Immune cells in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neonatal Respiratory Health Research
- RNA modifications and cancer
- Nanoplatforms for cancer theranostics
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Multiple Myeloma Research and Treatments
- Cancer, Lipids, and Metabolism
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Respiratory Support and Mechanisms
- RNA and protein synthesis mechanisms
- Histone Deacetylase Inhibitors Research
- TGF-β signaling in diseases
- Alcoholism and Thiamine Deficiency
- Porphyrin Metabolism and Disorders
- Wnt/β-catenin signaling in development and cancer
- Liver Disease Diagnosis and Treatment
Universidad de Navarra
2013-2025
Navarre Institute of Health Research
2017-2022
Champalimaud Foundation
2018
Foundation for Medical Research
2018
Centro de Investigación Biomédica en Red de Cáncer
2017
Chartered Institute of Management Accountants
2014
It is now obvious that the majority of cellular transcripts do not code for proteins, and a significant subset them are long non-coding RNAs (lncRNAs). Many lncRNAs show aberrant expression in cancer, some have been linked to cell transformation. However, underlying mechanisms remain poorly understood it unknown how sequences lncRNA dictate their function. Here we characterize function p53-regulated human LINC-PINT cancer. We find downregulated multiple types cancer acts as tumor suppressor...
Microfluidic devices are becoming mainstream tools to recapitulate in vitro the behavior of cells and tissues. In this study, we use microfluidic filled with hydrogels mixed collagen-Matrigel composition study migration lung cancer under different invasion microenvironments. We present design device, characterize morphologically mechanically quantitative image analysis measure H1299 adenocarcinoma experimental conditions. Our results show plasticity cell migration, which turns from...
The migration of cancer cells is highly regulated by the biomechanical properties their local microenvironment. Using 3D scaffolds simple composition, several aspects cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in context migration. However, a combined study these factors that more closely resemble complex microenvironment ECM still missing. Here, we present comprehensive, quantitative analysis role cell-ECM interactions within...
Locoregional failure (LRF) in breast cancer patients post-surgery and post-irradiation (IR) is linked to a dismal prognosis. In refined new model, we identified Enpp1 (Ectonucleotide pyrophosphatase /phosphodiesterase 1/CD203a) be closely associated with LRF. Enpp1high circulating tumor cells (CTC) contribute relapse by self-seeding mechanism. This process requires the infiltration of PMN-MDSC neutrophil extracellular traps (NET) formation. Genetic pharmacological inhibition or NET blockade...
Increasing interest has been devoted in recent years to the understanding of alternative splicing cancer. In this study, we performed a genome‐wide analysis identify cancer‐associated splice variants non‐small cell lung We discovered and validated novel differences genes known be relevant cancer biology, such as NFIB, ENAH or SPAG9. Gene enrichment analyses revealed an important contribution cancer‐related molecular functions, especially those involved cytoskeletal dynamics. Interestingly,...
Multiple myeloma (MM) progression and myeloma-associated bone disease (MBD) are highly dependent on marrow mesenchymal stromal cells (MSCs). MM-MSCs exhibit abnormal transcriptomes, suggesting the involvement of epigenetic mechanisms governing their tumor-promoting functions prolonged osteoblast suppression. Here, we identify widespread DNA methylation alterations marrow-isolated MSCs from distinct MM stages, particularly in Homeobox genes involved osteogenic differentiation that associate...
Prussian blue (PB) is a coordination polymer based on the Fe2+…CN…Fe3+ sequence. It an FDA-approved drug, intended for oral use at acidic pH of stomach and most intestine track. However, FDA approval, huge number papers proposed PB nanoparticles (PBnp) under "physiological conditions", meaning buffered 7.4 high saline concentration. While these report that PBnp are stable this pH, small describes instead degradation same or similar values, i.e. in 7-8 range. Here we give definitively clear...
Abstract Background The discovery of functionally relevant KRAS effectors in lung and pancreatic ductal adenocarcinoma (LUAD PDAC) may yield novel molecular targets or mechanisms amenable to inhibition strategies. Phospholipids availability has been appreciated as a mechanism modulate oncogenic potential. Thus, phospholipid transporters play functional role KRAS-driven oncogenesis. Here, we identified systematically studied the transporter PITPNC1 its controlled network LUAD PDAC. Methods...
Tumor organoids are powerful tools to study cancer. However, the clinical translation of organoid-based studies depends on how closely organoid scaffolding material recapitulates tumor extracellular matrix (ECM). We present a quantitative analysis effect scaffold composition phenotype, tissue remodeling, metabolism, and drug resistance pancreatic ductal adenocarcinoma (PDAC) organoids. grew PDAC within hydrogels made Matrigel, collagen-I, or mixture collagen-I Matrigel. Our results show that...
Transforming Growth Factor beta (TGF-β) acts as a tumor suppressor early in carcinogenesis but turns into promoter later disease stages. In fact, TGF-β is known inducer of integrin expression by cells which contributes to cancer metastatic spread and inhibition has been shown attenuate metastasis mouse models. However, carcinoma often become refractory TGF-β-mediated growth inhibition. Therefore identifying patients that may benefit from anti-TGF-β therapy requires careful selection. We...
Cancer related deaths are primarily due to tumor metastasis. To facilitate their dissemination distant sites, cancer cells develop invadopodia, actin-rich protrusions capable of degrading the surrounding extracellular matrix (ECM). We aimed determine whether β3 integrin participates in invadopodia formed by lung carcinoma cells, based on our previous findings specific TGF-β induction dependent metastasis animal models carcinoma. In this study, we demonstrate that form response exposure....
Airway infection by nontypeable Haemophilus influenzae (NTHi) associates to chronic obstructive pulmonary disease (COPD) exacerbation and asthma neutrophilic airway inflammation. Lipids are key inflammatory mediators in these conditions consequently, NTHi may encounter free fatty acids during persistence. However, molecular information on the interplay NTHi-free is limited, we lack evidence importance of such interaction infection. Maintenance outer membrane lipid asymmetry play an essential...
The analysis of circulating tumor cells (CTCs) in blood is a powerful noninvasive alternative to conventional biopsy. Inertial-based separation promising high-throughput, marker-free sorting strategy for the enrichment and isolation CTCs. Here, we present validate double spiral microfluidic device that efficiently isolates CTCs with fine-tunable cut-off value 9 μm range 2 μm. We designed based on computer simulations introduce novel, customized inertial force term, provide practical...
Collapsin response mediator protein 2 (CRMP2) is an adaptor that adds tubulin dimers to the growing tip of a microtubule. First described in neurons, it now considered ubiquitous intervenes processes such as cytoskeletal remodeling, synaptic connection and trafficking voltage channels. Mounting evidence supports CRMP2 plays essential role neuropathology and, more recently, cancer. We have previously positive correlation between nuclear phosphorylation poor prognosis lung adenocarcinoma...
The morphological changes that occur in the central nervous system of patients with severe acute intermittent porphyria (AIP) have not yet been clearly established. aim this work was to analyze brain involvement AIP without epileptic seizures or clinical posterior reversible encephalopathy syndrome, as well a mouse model receiving liver-directed gene therapy aimed at correcting metabolic disorder. We conducted neuroradiologic studies 8 severely affected (6 women) and 16 gender- age-matched...
We present a fully automatic approach to quantitatively analyze filopodia-based migration of fluorescent cells in 3D time-lapse series. The proposed method involves three steps. First, each frame the series is preprocessed using steerable filter and binarized obtain coarse segmentation cell shape. Second, sequence morphological filters applied on binary mask separate body from individual filopodia. Finally, their length estimated geodesic distance transform. validated lung adenocarcinoma...
Chronic obstructive pulmonary disease (COPD) patients undergo infectious exacerbations whose frequency identifies a clinically meaningful phenotype. Mouse models have been mostly used to separately study both COPD and the processes, but reliable model of frequent exacerbator phenotype is still lacking. Accordingly, we first established single bacterial exacerbation by nontypeable Haemophilus influenzae (NTHi) infection on mice with emphysema-like lesions. We characterized this combining...
Purpose or ObjectiveTo report initial response, acute treatment-related toxicity and patient-reported quality of life (QoL) after Single-Dose SBRT (SDRT) at a prescription dose 24Gy from randomized Phase II study patients with histologically proven intermediate-risk adenocarcinoma the prostate (NCCN definition). Material MethodsBetween November 2015 December 20 16, 30 hormone-naïve were enrolled in an IRB-approved prospectively phase to receive either 45Gy 5 consecutive daily fractions...
ABSTRACT Multiple myeloma (MM) progression and myeloma-associated bone disease (MBD) are highly dependent on the marrow (BM) microenvironment, in particular mesenchymal stromal cells (MSCs). MSCs from MM patients exhibit an abnormal transcriptional profile, suggesting that epigenetic alterations could be governing tumor-promoting functions of their prolonged osteoblast (OB) suppression MM. In this study, we analyzed DNA methylome BM-derived with monoclonal gammopathy undetermined...
<div>Abstract<p>Locoregional failure (LRF) in patients with breast cancer post-surgery and post-irradiation is linked to a dismal prognosis. In refined new model, we identified ectonucleotide pyrophosphatase/phosphodiesterase 1/CD203a (ENPP1) be closely associated LRF. ENPP1<sup>hi</sup> circulating tumor cells (CTC) contribute relapse by self-seeding mechanism. This process requires the infiltration of polymorphonuclear myeloid-derived suppressor neutrophil...
Supplementary Data from Tumor ENPP1 (CD203a)/Haptoglobin Axis Exploits Myeloid-Derived Suppressor Cells to Promote Post-Radiotherapy Local Recurrence in Breast Cancer