A5043029506- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- Vector-borne infectious diseases
- Insect and Pesticide Research
- Insect Resistance and Genetics
- Neurobiology and Insect Physiology Research
- Trypanosoma species research and implications
- Heme Oxygenase-1 and Carbon Monoxide
- Viral Infections and Vectors
- Malaria Research and Control
- Insect Pest Control Strategies
- Insect Utilization and Effects
- Hemoglobin structure and function
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Insect and Arachnid Ecology and Behavior
- Insect-Plant Interactions and Control
- Physiological and biochemical adaptations
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Environmental Toxicology and Ecotoxicology
- Aquaculture Nutrition and Growth
- Iron Metabolism and Disorders
- Advanced Nanomaterials in Catalysis
- Physical Activity and Health
Universidade Federal do Rio de Janeiro
2016-2025
Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular
2016-2025
Universidade de São Paulo
2005-2025
Instituto de Biologia do Exército
2019
Link Consulting
2018
Universidade Nova de Lisboa
2017
Universidade de Brasília
2015
Hospital de Base
2015
Instituto de Biotecnología y Biología Molecular
2013
Rio de Janeiro Federal Institute of Education, Science and Technology
2011
Heme is an ancient and ubiquitous molecule present in organisms of all kingdoms, composed atom iron linked to four ligand groups porphyrin. A high amount free heme, a potential amplifier the inflammatory response, characteristic feature diseases with increased hemolysis or extensive cell damage. Here we demonstrate that but not its analogs/precursors, induced tumor necrosis factor-alpha (TNF-alpha) secretion by macrophages dependently on MyD88, TLR4, CD14. The activation TLR4 heme...
The presence of bacteria in the midgut mosquitoes antagonizes infectious agents, such as Dengue and Plasmodium, acting a negative factor vectorial competence mosquito. Therefore, knowledge molecular mechanisms involved control microbiota could help development new tools to reduce transmission. We hypothesized that toxic reactive oxygen species (ROS) generated by epithelial cells bacterial growth Aedes aegypti, vector Yellow fever viruses. show ROS are continuously present sugar-fed (SF)...
The bloodsucking hemipteran Rhodnius prolixus is a vector of Chagas' disease, which affects 7–8 million people today in Latin America. In contrast to other hematophagous insects, the triatomine gut compartmentalized into three segments that perform different functions during blood digestion. Here we report analysis transcriptomes for each using pyrosequencing technology. Comparison transcript frequency digestive libraries with whole-body library was used evaluate expression levels. All...
Abstract The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor symbiont bacterium the genus Coxiella . It was showed endosymbiont from R (CERM) vertically transmitted mutualist symbiont, comprising 98% 16S rRNA sequences both eggs and larvae. Sequencing bacterial genome revealed genes for biosynthetic pathways several vitamins key metabolic cofactors may provide nutritional complement to...
Haem and iron homeostasis in most eukaryotic cells is based on a balanced flux between haem biosynthesis oxygenase-mediated degradation. Unlike eukaryotes, ticks possess an incomplete biosynthetic pathway and, together with other (non-haematophagous) mites, lack gene encoding oxygenase. We demonstrated, by membrane feeding, that do not acquire bioavailable from haemoglobin-derived haem. However, require dietary haemoglobin as exogenous source of since, feeding haemoglobin-depleted serum led...
Technologies based on RNA interference may be used for insect control. Sustainable strategies are needed to control vectors of Chagas disease such as Rhodnius prolixus. The microbiota can modified deliver molecules the gut. Here, Escherichia coli HT115(DE3) expressing dsRNA heme-binding protein (RHBP) and catalase (CAT) were fed nymphs adult triatomine stages. RHBP is an egg CAT antioxidant enzyme expressed in all tissues by developmental effect was systemic temporal. Concentrations E. above...
SUMMARY Heme is present in all cells, acting as a cofactor essential metabolic pathways such respiration and photosynthesis. Moreover, both heme its degradation products, CO, iron biliverdin, have been ascribed important signaling roles. However, limited knowledge available on the intracellular involved flux of between different cell compartments. The cattle tick Boophilus microplus ingests 100 times own mass blood. digest cells midgut endocytose blood components huge amounts are released...
Hemozoin (Hz) is a heme crystal produced upon the digestion of hemoglobin (Hb) by blood‐feeding organisms as main mechanism disposal. The structure Hz consists dimers bound reciprocal iron–carboxylate interactions and stabilized hydrogen bonds. We have recently described crystals in blood fluke, Schistosoma mansoni, kissing bug, Rhodnius prolixus . Here, we characterized structures morphologies from those two compared them to synthetic β‐hematin (βH). Synchrotron radiation X‐ray powder...
Aedes aegypti is the main vector of important arboviruses such as dengue, Zika and chikungunya. During infections mosquitoes can activate immune pathways Toll, IMD JAK/STAT to limit pathogen replication. Here, we evaluate response profile Ae. against Sindbis virus (SINV). We analyzed gene expression components showed that a blood meal infection upregulated aaREL2 in microbiota-dependent fashion, since this induction was prevented by antibiotic. The presence microbiota activates impaired...
Background Digestion of blood in the midgut Aedes aegypti results release pro-oxidant molecules that can be toxic to mosquito. We hypothesized after a meal, antioxidant capacity is increased protect cells against oxidative stress. Concomitantly, pathogens present ingested by mosquitoes, such as arboviruses Dengue and Zika, also have overcome same challenge, program induced insect likely influence infection status mosquito its vectorial competence. Methodology/Principal findings found...
Aedes aegypti is the vector of some most important vector-borne diseases like dengue, chikungunya, zika and yellow fever, affecting millions people worldwide. The cellular processes that follow a blood meal in mosquito midgut are directly associated with pathogen transmission. We studied homeostatic response against oxidative stress, as well bacterial dengue virus (DENV) infections, focusing on proliferative ability intestinal stem cells (ISC). Inhibition peritrophic matrix (PM) formation...
NADPH Oxidase 5 (Nox5) is a calcium-sensitive superoxide-generating Nox. It present in lower forms and higher mammals, but not rodents. Nox5 expressed vascular cells, the functional significance remains elusive. Given that contraction controlled by calcium reactive oxygen species, both associated with Nox5, we questioned role of pro-contractile signaling function.
Culture-independent metagenomic methodologies have enabled detection and identification of microorganisms in various biological systems often revealed complex unknown microbiomes. In many organisms, the microbiome outnumbers host cells greatly affects biology fitness. Ticks are hematophagous ectoparasites with a wide range. They vector number human animal pathogens also directly cause major economic losses livestock. Although several reports on tick midgut microbiota show diverse bacterial...
SUMMARY The hard tick Boophilus microplus ingests large volumes of cattle blood, as much 100 times its own mass before feeding. Huge amounts haem are produced during haemoglobin digestion, which takes place inside acidic lysosomal-type vacuoles the digest cells midgut. Haem is a promoter free radical formation, so digestion poses an intense oxidative challenge to this animal. In present study we followed fate derived from hydrolysis in midgut fully engorged females. does not synthesize haem,...
The main protein of the hemolymph cattle tick <i>Boophilus microplus</i> has been isolated and shown to be a heme lipoprotein (HeLp). HeLp an apparent molecular mass 354,000 contains two apoproteins (103 92 kDa) found in equal amounts. presents pI 5.8 density 1.28 g/ml 33% lipids, containing both neutral lipids phospholipids, 3% sugars. A remarkable feature is abundance cholesterol ester (35% total lipids), lipid not previously reported invertebrate lipoproteins. Western blot analysis showed...
The gene Aedes aegypti intestinal mucin 1 (AeIMUC1) encodes a putative peritrophic matrix (PM) protein that is expressed in the midgut of mosquito larvae and adults upregulated response to exposure heavy metals. AeIMUC1 has predicted secretory signal peptide three chitin-binding domains (CBDs) with an intervening mucin-like domain. Immunofluorescence immunoelectron microscopy experiments established bona fide PM protein, binding recombinant chitin was demonstrated vitro. Previous suggested...