A5001984770- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Retinoids in leukemia and cellular processes
- Mosquito-borne diseases and control
- Retinal Development and Disorders
- Plant Virus Research Studies
- Aquaculture disease management and microbiota
- Neurobiology and Insect Physiology Research
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- interferon and immune responses
- Corneal Surgery and Treatments
- CRISPR and Genetic Engineering
- NF-κB Signaling Pathways
- Fibroblast Growth Factor Research
- Hair Growth and Disorders
- Neonatal Health and Biochemistry
- Genomics and Chromatin Dynamics
- Developmental Biology and Gene Regulation
- Reproductive System and Pregnancy
- Signaling Pathways in Disease
- Bacteriophages and microbial interactions
- Historical Medical Research and Treatments
- Congenital Ear and Nasal Anomalies
- Toxin Mechanisms and Immunotoxins
Institut de Biologie Moléculaire et Cellulaire
2010-2024
Centre National de la Recherche Scientifique
2003-2022
Université de Strasbourg
2010-2022
Institut de Biologie Moléculaire des Plantes
2017-2018
Modèles Insectes de l'Immunité Innée
2017-2018
University of Basel
2012
Institut de génétique et de biologie moléculaire et cellulaire
2003-2005
Institut Clinique de la Souris
2003-2005
Collège de France
2003-2005
Inserm
2003-2005
The retinoic acid (RA) signal, produced locally from vitamin A by retinaldehyde dehydrogenase (Raldh) and transduced the nuclear receptors for retinoids (RA receptor 9- cis -RA receptor), is indispensable ontogenesis homeostasis of numerous tissues. We demonstrate that Raldh3 knockout in mouse suppresses RA synthesis causes malformations restricted to ocular nasal regions, which are similar those observed A-deficient fetuses and/or retinoid mutants. notably choanal atresia (CA), responsible...
Using genetic approaches in the mouse, we show that primary target tissue of retinoic acid (RA) action during eye morphogenesis is not retina nor corneal ectoderm, which both express RA-synthesizing retinaldehyde dehydrogenases (RALDH1 and RALDH3), but neural crest cell-derived periocular mesenchyme (POM), devoid RALDH. In POM, effects paracrine RA signal are mediated by nuclear receptors heterodimers RXRα/RARβ RXRα/RARγ. These appear to control: (1) remodeling POM through activation...
Microbial or endogenous molecular patterns as well pathogen functional features can activate innate immune systems. Whereas detection of infection by pattern recognition receptors has been investigated in details, sensing virulence factors activities remains less characterized. In Drosophila, genetic evidences indicate that the serine protease Persephone belongs to a danger pathway activated abnormal proteolytic induce Toll signaling. However, neither activation mechanism this nor its...
Thioester-containing proteins (TEPs) are conserved among insects that thought to be involved in innate immunity. In <i>Drosophila</i>, the <i>Tep</i> family is composed of 6 genes named <i>Tep1–Tep6</i>. this study, we investigated phylogeny, expression pattern and roles these host defense <i>Drosophila</i>. Protostomian<i> Tep </i>genes clustered 3 distinct branches, 1 which specific mosquitoes. Most <i>D. melanogaster...
The network of NF-κB-dependent transcription that activates both pro- and anti-inflammatory genes in mammals is still unclear. As NF-κB factors are evolutionarily conserved, we used Drosophila to understand this network. factor Relish effector gene expression following Gram-negative bacterial immune challenge. Here, show, using a genome-wide approach, the conserved nuclear protein Akirin co-factor required for activation subset Relish-dependent correlating with presence H3K4ac epigenetic...
Chronic inflammation of the intestine is detrimental to mammals. Similarly, constant activation immune response in gut by endogenous flora suspected be harmful Drosophila . Therefore, innate melanogaster tightly balanced simultaneously prevent infections pathogenic microorganisms and tolerate flora. Here we describe role big bang ( bbg ) gene, encoding multiple membrane-associated PDZ (PSD-95, Discs-large, ZO-1) domain-containing protein isoforms, modulation response. We show that adult...
Fusion and hypoplasia of the first two branchial arches, a defect typically observed in retinoic acid (RA) embryopathy, is generated cultured mouse embryos upon treatment with BMS453, synthetic compound that exhibits receptor β (RARβ) agonistic properties transfected cells. By contrast, no arch defects are following retinoids exhibit RARα or RARγ properties. The BMS453-induced mediated through RAR activation, as they similar to those by selective pan-RAR agonist, prevented antagonist cannot...
Mouse limb bud is a prime model to study the regulatory interactions that control vertebrate organogenesis. Major aspects of development are controlled by feedback loops define self-regulatory signalling system. The SHH/GREM1/AER-FGF loop forms core this system operates between posterior mesenchymal organiser and ectodermal centre. BMP antagonist Gremlin1 (GREM1) critical node in system, whose dynamic expression BMP, SHH, FGF key normal progression development. Previous analysis identified...
Within cells, retinol (ROL) is bound to cytoplasmic proteins (cellular retinol-binding [CRBPs]), whose proposed function protect it from unspecific enzymes through channeling retinoid-metabolizing pathways. We show that, during development, ROL and retinyl ester levels are decreased in CRBP type 1 (CRBP1) -deficient embryos fetuses by 50% 80%, respectively. The steady state level of retinoic acid (RA) also but a lesser extent. However, CRBP1-null do not exhibit the abnormalities...
The Immune Deficiency (IMD) pathway in Drosophila melanogaster is activated upon microbial challenge with Gram-negative bacteria to trigger the innate immune response. In order decipher this nuclear factor κB (NF-κB) signaling pathway, we undertook an vitro RNAi screen targeting E3 ubiquitin ligases specifically and identified HECT-type ligase Hyperplastic discs (Hyd) as a new actor IMD pathway. Hyd mediated Lys63 (K63)-linked polyubiquitination of NF-κB cofactor Akirin was required for...
The ability of metazoans to combat pathogenic infection involves both systemic and local responses the invading pathogens. Ubiquitin SUMO pathways molecularly regulate response infection, immune signaling gene expression. Here, we report that Degringolade (Dgrn, CG10981), a SUMO-targeted ubiquitin ligase connecting two pathways, is essential for innate immunity in Drosophila. dgrnDK null heterozygous mutant adult flies are severely immune-compromised succumb rapidly bacteria fungi...
The evolutionarily conserved immune deficiency (IMD) signaling pathway shields Drosophila against bacterial infections. It regulates the expression of antimicrobial peptides encoding genes through activation NF-κB transcription factor Relish. Tight regulation cascade ensures a balanced response, which is otherwise highly harmful. Several phosphorylation events mediate intracellular progression IMD pathway. However, signal termination by dephosphorylation remains largely elusive. Here, we...
One Sentence Summary Upon microbial infection in Drosophila , the E3-ubiquitin ligase Hyd ubiquitinylates NF-κB co-factor Akirin for its efficient binding to factor Relish and subsequent activation of immune effectors genes. ABSTRACT The IMD pathway is activated upon challenge with Gramnegative bacteria trigger innate response. In order decipher this signaling pathway, we undertook an ex-vivo RNAi screen targeting specifically E3 ubiquitin ligases identified HECT Hyperplastic Discs “Hyd” as...