A5102978887- Receptor Mechanisms and Signaling
- Photochromic and Fluorescence Chemistry
- Hedgehog Signaling Pathway Studies
- Monoclonal and Polyclonal Antibodies Research
- Axon Guidance and Neuronal Signaling
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Machine Learning in Bioinformatics
- Chemical Synthesis and Analysis
ShanghaiTech University
2017-2019
Shanghai Institute of Materia Medica
2017
Chinese Academy of Sciences
2017
University of Chinese Academy of Sciences
2017
Abstract The Smoothened receptor (SMO) belongs to the Class Frizzled of G protein-coupled (GPCR) superfamily, constituting a key component Hedgehog signalling pathway. Here we report crystal structure multi-domain human SMO, bound and stabilized by designed tool ligand TC114, using an X-ray free-electron laser source at 2.9 Å. reveals precise arrangement three distinct domains: seven-transmembrane helices domain (TMD), hinge (HD) intact extracellular cysteine-rich (CRD). This architecture...
Inspired by recent progress in structural studies of the smoothened receptor (SMO), a group affinity probes were developed to specifically light up SMO grafting small fluorescent at specific residue K395.
We present herein a novel dissection-then-evolution strategy for ligand optimization.
Membrane mimics are indispensable tools in the structural and functional understanding of membrane proteins (MPs). Given stringent requirements integral MP manipulations, amphiphile replacement is often required sample preparation for various biophysical purposes. Current protocols generally rely on physical methodologies rarely reach complete replacement. In comparison, we report herein a chemical alternative that facilitates exhaustive exchange membrane-mimicking systems reconstitution....