A5037033982- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- Long-Term Effects of COVID-19
- SARS-CoV-2 detection and testing
- Viral Infections and Vectors
- Sirtuins and Resveratrol in Medicine
- Animal Virus Infections Studies
- Inflammasome and immune disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Neonatal and fetal brain pathology
- interferon and immune responses
- Influenza Virus Research Studies
- HIV Research and Treatment
- Endoplasmic Reticulum Stress and Disease
- Vibrio bacteria research studies
- Malaria Research and Control
Georgia State University
2019-2025
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection can cause neurological disease in humans, but little is known about the pathogenesis of SARS-CoV-2 central nervous system (CNS). Herein, using K18-hACE2 mice, we demonstrate that neuroinvasion and encephalitis associated with mortality these mice. Intranasal mice 105 plaque-forming units resulted 100% by day 6 after infection. The highest virus titers lungs were observed on 3 declined days 5 By contrast, very high levels...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the current pandemic, resulting in millions of deaths worldwide. Increasingly contagious variants concern (VoC) have fueled recurring global infection waves. A major question is relative severity disease by previous and currently circulating SARS-CoV-2. In this study, we evaluated pathogenesis SARS-CoV-2 human ACE-2-expressing (K18-hACE2) mice. Eight-week-old K18-hACE2 mice were inoculated intranasally with a...
Abstract Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection can cause neurological disease in humans, but little is known about the pathogenesis of SARS-CoV-2 central nervous system. Herein, using K18-hACE2 mice, we demonstrate that neuroinvasion and encephalitis associated with mortality these mice. Intranasal mice 10 5 plaque-forming units resulted 100% by day 6 after infection. The highest virus titers lungs were observed at 3 declined days In contrast, very high...
Transgenic mice expressing human angiotensin-converting enzyme 2 under the cytokeratin 18 promoter (K18-hACE2) have been extensively used to investigate pathogenesis and tissue tropism of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Neuroinvasion replication SARS-CoV-2 within central nervous system (CNS) K18-hACE2 is associated with increased mortality; although, mechanisms by which this occurs remain unclear. In study, we generated primary neuronal cultures from...
West Nile virus (WNV) is a flavivirus that has disseminated globally as significant cause of viral encephalitis in humans. MircoRNA-155 (miR-155) regulates various aspects innate and adaptive immune responses. We previously reported WNV infection induces upregulation miR-155 mice brains. In the current study, we demonstrate critical role restricting pathogenesis mice. Compared to wild-type (WT) mice, knockout exhibited significantly higher morbidity mortality after with either lethal strain,...
WWest Nile virus (WNV), a neurotropic flavivirus, is the leading cause of viral encephalitis in United States. Recently, ZIKV infections have caused serious neurological diseases and birth defects, specifically Guillain-Barrè syndrome microcephaly. Z-DNA binding protein 1 (ZBP1) cytoplasmic sensor that has been shown to play significant role initiating robust immune response. We previously reported WNV induce dramatic up-regulation ZBP1 mouse brain as well infected primary cells. Herein, we...
The emergence of new severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants concern pose a major threat to public health, due possible enhanced virulence, transmissibility and immune escape. These may also adapt hosts, in part through mutations the spike protein. In this study, we evaluated infectivity pathogenicity SARS-CoV-2 wild-type C57BL/6 mice. Six-week-old mice were inoculated intranasally with representative virus from original B.1 lineage, or emerging B.1.1.7 B.1.351...
Neurological manifestations are a significant complication of coronavirus disease 2019 (COVID-19), but the underlying mechanisms yet to be understood. Recently, severe acute respiratory syndrome 2 (SARS-CoV-2)-induced neuroinvasion and encephalitis were observed in K18-hACE2 mice, leading mortality. Our goal this study was gain insights into molecular pathogenesis neurological mouse model. To analyze differentially expressed genes (DEGs) brains mice following SARS-CoV-2 infection, we...
Widespread and rapidly evolving SARS-CoV-2 posed an unprecedented challenge to vaccine developers. GeoVax has designed a multiantigen vaccine, designated GEO-CM02 based on Modified Vaccinia Virus (MVA) vector that expresses spike (S), membrane (M), envelope (E) antigens. This experimental was tested in the hACE2 transgenic mouse model assess immunogenicity efficacy. Administration of two-dose regimen elicited high levels neutralizing antibodies provided complete protection, effectively...
Physiological blood-tissue barriers play a critical role in separating the circulation from immune-privileged sites and denying access to blood-borne viruses. The mechanism of virus restriction by these is poorly understood. We utilize induced pluripotent stem cell (iPSC)-derived human brain microvascular endothelial cells (iBMECs) study virus-blood-brain barrier (BBB) interactions. These iPSC-derived faithfully recapitulate striking difference vivo neuroinvasion two alphavirus isolates are...
Summary SARS-COV-2 has recently emerged as a new public health threat. Herein, we report that the FDA-approved gold drug, auranofin, inhibits replication in human cells at low micro molar concentration. Treatment of with auranofin resulted 95% reduction viral RNA 48 hours after infection. Auranofin treatment dramatically reduced expression SARS-COV-2-induced cytokines cells. These data indicate could be useful drug to limit SARS-CoV-2 infection and associated lung injury due its anti-viral,...
Abstract The emergence of new severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants concern poses a major threat to the public health due possible enhanced virulence, transmissibility and immune escape. These may also adapt hosts in part through mutations spike protein. In this study, we evaluated infectivity pathogenicity SARS-CoV-2 wild-type C57BL/6 mice. Six-week-old mice were inoculated intranasally with representative virus from original B.1 lineage or emerging B.1.1.7...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic resulting in millions of deaths worldwide. Increasingly contagious variants concern (VoC) have fueled recurring global infection waves. A major question is the relative severity disease by previous and currently circulating SARS-CoV-2. In this study, we evaluated pathogenesis SARS-CoV-2 human ACE-2-expressing (K18-hACE2) mice. Eight-week-old K18-hACE2 mice were inoculated intranasally with...
The emergence of new SARS-CoV-2 variants continues to cause challenging problems for the effective control COVID-19. In this study, we tested hypothesis whether a strategy multivalent and sequential heterologous spike protein vaccinations would induce broader range higher levels neutralizing antibodies against more protection than homologous vaccination in mouse model. We determined spike-specific IgG, receptor-binding inhibition titers, protective efficacy groups mice that were vaccinated...
West Nile virus (WNV) and Japanese encephalitis (JEV) are emerging mosquito-borne flaviviruses causing globally. No specific drug or therapy exists to treat flavivirus-induced neurological diseases. The lack of therapeutics underscores an urgent need determine the function important host factors involved in flavivirus replication disease progression. Interleukin-6 (IL-6) upregulation has been observed during viral infections both mice humans, implying that it may influence outcome...