A5045210418- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Cytomegalovirus and herpesvirus research
- Escherichia coli research studies
- Mosquito-borne diseases and control
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Vibrio bacteria research studies
University of Illinois Urbana-Champaign
2022-2024
Goodwin College
2024
Louisiana State University in Shreveport
2022
The perpetual arms race between bacteria and their viruses (phages) has given rise to diverse immune systems, including restriction-modification CRISPR-Cas, which sense degrade phage-derived nucleic acids. These complex systems rely upon production maintenance of multiple components achieve antiphage defense. However, the prevalence effectiveness minimal, single-component that cleave DNA remain unknown. Here, we describe a unique mode acid immunity mediated by single enzyme with nuclease...
Clustered regularly-interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins protect bacteria archaea from their viruses, anti-CRISPRs (Acrs) are small virus-encoded that inhibit CRISPR-Cas immunity. Over 80 families of Acrs have been described to date; however, only three these subvert Type III systems employ a complex network Cas accessory nucleases degrade viral nucleic acids. Here, we discover characterize AcrIIIA1, the first III-A specific anti-CRISPR...
Human cytomegalovirus (HCMV) exhibits a complex host-pathogen interaction with peripheral blood monocytes. We have identified unique, cell-type specific retrograde-like intracellular trafficking pattern that HCMV utilizes to gain access the monocyte nucleus and for productive infection. show infection of primary human monocytes, epithelial cells, fibroblasts leads an increase in amount protein Syntaxin 6 (Stx6). However, only knockdown (KD) Stx6 monocytes inhibited viral trans-Golgi network...