A5022082847- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sexual Differentiation and Disorders
- Herpesvirus Infections and Treatments
- Animal Genetics and Reproduction
- Parvovirus B19 Infection Studies
- Reproductive Health and Technologies
- Renal and related cancers
- Birth, Development, and Health
- Genetic Syndromes and Imprinting
- Cytomegalovirus and herpesvirus research
- Digital Transformation in Law
- Prenatal Screening and Diagnostics
- Human Health and Disease
- Reproductive System and Pregnancy
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- LGBTQ Health, Identity, and Policy
- thermodynamics and calorimetric analyses
- Genetically Modified Organisms Research
- Insect and Pesticide Research
- Plant Genetic and Mutation Studies
- Testicular diseases and treatments
- Medical and Agricultural Research Studies
Research Centre for Medical Genetics
2005-2022
Research Institute of Medical Genetics of Russian Academy of Medical Sciences
1981-2015
Russian Academy of Sciences
2002-2014
Academy of Medical Sciences
1998-2011
D.I. Ivanovsky Institute of Virology Russian Academy of Medical Sciences
2007
Ministry of Health of the Russian Federation
1984-1999
Medical Radiological Research Center
1998
Moscow State University
1998
Physico-Technical Institute
1972
The presence of human cytomegalovirus (HCMV) in male genital tract suggests its vertical transmission with spermatozoa and the development a potentially dangerous fetal infection. objective present study was to evaluate possibility intracellular HCMV localization germ cells examine effect virus on spermatogenesis. Semen samples from 91 infertile 47 fertile men were analyzed. detected by real time PCR, rapid culture method PCR situ. Human testis organotypic quantitative karyological analysis...
Summary Acute and chronic infections of the seminal tract are among most common causes male infertility. As at least half infertility cases classified as idiopathic, some these might be attributed to asymptomatic infection. The detection quantification Epstein–Barr virus ( EBV ), cytomegalovirus CMV ) human herpes type 6 HHV ‐6) DNA in semen samples were performed. A total 232 patients divided into five groups: (i) infertile men with varicocoele; (ii) idiopathic infertility; (iii)...
ABSTRACT Growth, morphogenesis and cell movements were studied in Obelia loveni, O. geniculata Dynamena pumila with the use of time-lapse cinematography, visual observations vitally stained objects by histological techniques. Growth pulsations period around 14 min amplitude 15 μm exist 5–8 up to 5 loveni. It was demonstrated that rhythm growth does not coincide periodical contractions proximal part coenosarc. The distalwards individual cells ectoderm growing stems hydranth rudiments are...
The fibrous sheath is a unique cytoskeletal structure surrounding the axoneme and outer dense fibres of sperm flagellum. Dysplasia (DFS) defect spermatozoa observed in severe asthenozoospermic patients characterised by morphologically abnormal flagella with distorted sheaths. Sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) glycolytic enzyme that tightly associated enzymatic activity GAPDS was investigated samples seven DFS compared to normal (n = 10). difference statistically...
Genetic factors (chromosomal aberrations and point mutations) are the cause of infertility in 10–15 % men with impaired fertility. Homogeneous structural functional defects sperm or total terato-, asthenozoospermia – rare cases genetically determined male infertility, autosomal recessive diseases. Currently, described 4 types «syndromic» spermopatology. 1. Primary ciliary dyskinesia (PCD) asthenozoospermia. Affects axoneme structures (microtubules, dynein arms, radial spokes). It identified...
We report on a 37-year-old XX male with complex hidden X chromosomal mosaicism. The patient had fully mature genitalia hypoplastic testes descended in the scrotum and no sign of undervirilization. Hormonal examination demonstrated hypergonadotropic hypogonadism, semen analysis showed severe oligoasthenoteratozoospermia. In situ hybridization revealed presence 3 SRY-positive cell lines bearing 1, 2 or chromosomes. Skewed inactivation paternal SRY-bearing chromosome was detected by molecular...
This review describes folliculogenesis from the formation of a primordial follicle around oocyte during diplotene stage prophase meiosis I to that preovulatory follicle, maturation an oocyte, and transformation its chromosomal nucleolus complex into karyosphere. It briefly highlights literature disagreements on terminology ovarian follicular reserve folliculogenesis. The possibilities evaluating system are given.
We report on a 45,X male with hydrocephaly, lobar holoprosencephaly and ichthyosis. In situ hybridization molecular analysis have demonstrated the presence of mosaic SRY-bearing derivative X chromosome that included Yp heterochromatic Yq fragments.
Введение. В последнее десятилетие появилось понимание двоякой природы повреждения генетического аппарата сперматозоидов: нарушение конденсации хроматина («незрелый» хроматин, НХ), связанное с нарушенной протаминизацией и приводящее к нарушению эпигенетической регуляции раннего эмбриогенеза, целостности ДНК сперматозоидов – фрагментация (ФДС).Цель исследования изучение корреляции между нарушением ФДС.Материалы методы. Проводили исследование 54 фертильных мужчин (1-я группа, контрольная), 46...
Sperm DNA integrity is an essential factor for successful fertilization and proper pregnancy progression. The terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) assay commonly used the estimation of fragmentation index. Analysis TUNEL-stained sperm often performed by flow cytometry, approach that allows high-throughput analysis but in which any morphological information lost. In this study, results automated image cytometry sperms were presented. visual counting automatic...
Sperm cells DNA fragmentation is one of the factors male sub-/infertility discovered recently. At present, pathophysiological mechanisms that cause have not been studied completely. It suggested that they may be caused with defects chromatin remodeling, apoptosis, and oxidative stress. Spermiological examination was performed in 461 infertile men. With 23 % patients examined, frequency sperm comprises over 15 %, that, 18 patients demonstrated its range from 15.1 to 30 5 patients, it exceeded...
Genetic and epigenetic mechanisms of spermatogenesis – long process with many stages regulation are discussed. DNA code is the entirety hereditary information, gene act without altering primary nucleotide sequences. Epigenetic a complex process, in which components different groups modifications (non-coding RNAs, methylation histone modification) work together. Mistakes any may cause impaired and/or infertility, diseases. Nowadays 90 imprinted genes loci on 13 chromosomes revealed. More then...