A5046946365- Parkinson's Disease Mechanisms and Treatments
- Glaucoma and retinal disorders
- Neurological disorders and treatments
- Nuclear Receptors and Signaling
- Lysosomal Storage Disorders Research
- Genetic Neurodegenerative Diseases
- Growth Hormone and Insulin-like Growth Factors
- Trace Elements in Health
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Neurological diseases and metabolism
- Autism Spectrum Disorder Research
- Genetic Syndromes and Imprinting
- Cancer-related gene regulation
- Infrared Thermography in Medicine
- Hemophilia Treatment and Research
- Alzheimer's disease research and treatments
- Pancreatic function and diabetes
- RNA regulation and disease
- Oral and Maxillofacial Pathology
- Adenosine and Purinergic Signaling
- Virus-based gene therapy research
- Oral Health Pathology and Treatment
- Heavy Metal Exposure and Toxicity
- Biochemical and Molecular Research
University of Calcutta
2014-2023
P. D. Hinduja Hospital and Medical Research Centre
2022
National Hospital
2022
Burdwan Medical College & Hospital
2019
Medical College and Hospital, Kolkata
2019
Christie's
2019
Dr. R. Ahmed Dental College and Hospital
2011-2017
Yahoo (United Kingdom)
2010-2011
Cornell University
1997-2005
New York State College of Veterinary Medicine
2005
The human growth hormone-variant (hGH-V) gene is one of five highly similar hormone-related genes clustered on the short arm chromosome 17. Although pattern expression adjacent normal hormone (hGH-N) and chorionic somatomammotropin (hCS) in this cluster are well characterized, hGH-V remains to be defined. In previous studies, we have demonstrated that transcribed term placenta expressed as two alternatively spliced mRNAs: predicted encode a 22-kD (hGH-V), other retains intron 4 its sequence...
Stably transfected cell lines containing the normal human growth hormone (hGH-N) and hormone-variant (hGH-V) genes have been established in order to study expression of these two highly homologous genes. Each gene was inserted into a bovine papillomavirus shuttle vector under transcriptional control mouse metallothionein promoter resultant recombinants were C127 cells. The cells hGH-N secrete hGH proteins, 91% migrating at 22 kD 9% 20 kD, same relative proportions synthesized vivo by...
The human GH-variant (hGH-V) gene, a member of the GH-PRL gene family, is expressed by placenta during second and third trimesters gestation. secreted hGH-V protein differs from pituitary GH (hGH-N) only 13 amino acids. We have previously demonstrated that can bind to both somatogen lactogen cell surface receptors in vitro, but ratio its receptorbinding affinities substantially higher than hGH-N. now characterize bioactivities contrast them bioactivity Somatogen was assayed stimulation...
Wilson disease (WD) is an autosomal-recessive disorder caused by mutations in the copper (Cu)-transporter ATP7B. Thus far, studies of WD have been limited to analysis ATP7B mutants homozygous states. However, majority patients are compound-heterozygous, and how different on two alleles impact properties unclear. We characterized five identified Indian patients, first expressing each alone then co-expressing with dissimilar properties. Mutations located regulatory domains ATP7B-A595T, S1362A,...
Wilson’s disease (WD) is a rare autosomal recessive copper metabolism disorder that primarily affects hepatic and neuronal tissues. The condition caused by mutations in the ATP7B gene. Our group conducted extensive molecular genetic studies, identifying 13 clinically diagnosed Indian WD patients lacking coding variant of 17 with single mutated allele. We hypothesize these patients, unidentified may reside cis-regulatory elements or WD-like phenotype results from cumulative effect...
Mucopolysaccharidosis type VII (MPS VII) is an inherited disease resulting from deficient activity of the lysosomal acid hydrolase beta-glucuronidase (GUSB) and has been reported in humans, mice, cats, dogs. To characterize canine MPS VII, we have isolated sequenced GUSB cDNA normal affected animals. A single nucleotide substitution was detected derived This guanosine to adenine base change at position 559 sequence causes arginine histidine amino 166. Introduction G a mammalian expression...
Genes for normal human pituitary GH (hGHN) and the variant (hGH-V) were expressed in stably transfected mouse mammary cells. The biological properties of hGH-N hGH-V secreted into medium examined using Tat adipocytes or epididymal fat segments. MethionylhGH produced E. coli served as a reference standard. three preparations quite similar their ability to bind specifically intact cells virtually indistinguishable increase glucose oxidation (an insulin-like response), induce refractoriness...
We have recently established that the human growth hormone-variant (hGH-V) gene is functional in vivo by documenting its expression placenta. subsequently generated transformed murine cell lines stably expressing genes for normal pituitary hormone (hGH-N), hGH-V, and each of two chimeric exon 3 exchanges, hGH-NV3 hGH-VN3. In present study, we utilize these as sources to characterize compare receptor binding profiles hGH-N with hGH-V. hGH-V was found displace 125I-ovine prolactin bound rat...
The human growth hormone variant (hGH-V) gene is expressed by the syncytiotrophoblastic layer of placenta in two forms: hGH-V mRNA encoding a 22 kD protein, and hGH-V2 which retains intron 4 expected to encode 26 protein. There predicted N-linked glycosylation site at amino acid 140 that absent both highly homologous normal pituitary GH (hGH-N). Cell lines transfected with hGH-N secrete 20 product an alternatively spliced mRNA, while cell three proteins 22, 24, kD. To determine whether any...
// Bhaskar Saha 1, 2, * , Birija Sankar Patro 3, 4, Mrunesh Koli 3 Ganesh Pai Jharna Ray 2 Sandip K. Bandyopadhyay 1 and Subrata Chattopadhyay 4 Vijaygarh Jyotish College, Jadavpur, Kolkata 700 032, India S. N. Pradhan Centre for Neuroscience, Ballygunge Science University of Calcutta, 019, Bio-Organic Division, Bhabha Atomic Research Centre, Mumbai 400085, Homi National Institute, Training School Complex, Anushakti Nagar, 400094, These authors have contributed equally to this work...
Human GH-variant (hGH-V) is a natural GH analog arising from the hGH-V gene. It expressed in placenta and secreted into maternal circulation during second half of pregnancy. To gain information about its bioactivity man, we examined interaction with high affinity GH-binding protein/receptor (GH-BP) human plasma. was equipotent pituitary hGH (hGH-N) as ligand for GH-BP. hGH-N/hGH-V chimeric proteins, where sequences encoded by exon 3 (amino acid residues 32–71, thought to be exposed on...
Parkinson's disease (PD) is a common neurodegenerative movement disorder. Among the candidate genes, DJ-1 accounts for about 1% of cases in different populations. We aim to find contribution gene towards PD among Indians. By screening 308 patients eastern India and 248 ethnically matched controls, total 21 nucleotide variants - including two nonsynonymous changes were detected. p.Arg98Gln was identified 6 unrelated 2 controls while p.Val35Ile, novel change, found only patients. A SNP...
Therapeutic hypothermia has proven benefits in critical care of a number diseased states, where inflammation and oxidative stress are the key players. Here, we report that adenosine monophosphate (AMP) triggered hypometabolic state (HMS), 1-3 hours after lethal total body irradiation (TBI) for duration 6 hours, rescue mice from radiation-induced lethality this effect is mediated by persistent hypothermia. Studies with caffeine 6N-cyclohexyladenosine, non-selective antagonist selective...
Glaucoma is the largest cause of irreversible blindness affecting more than 60 million people globally. The disease defined as a gradual loss peripheral vision due to death Retinal Ganglion Cells (RGC). RGC largely influenced by rate aqueous humor production ciliary processes and its passage through trabecular meshwork (TM) in anterior part eye. Primary open angle glaucoma (POAG), most common subtype, genetically complex disease. Multiple genes many loci have been reported be involved POAG...
Dystonia is a common movement disorder. The purpose of this study to examine the relative distribution primary dystonia subtypes and identify mutation (s) in DYT1 gene Indian patients.Primary patients (n = 178) controls 63), lacking any symptoms disease, were recruited for from eastern India. nucleotide variants identified by carrying out polymerase chain reaction, single stranded conformation polymorphism, DNA sequencing.Unlike other reports, pain and/or tremor was more our sporadic than...