A5032499255- Ultrasound and Hyperthermia Applications
- Kidney Stones and Urolithiasis Treatments
- Pediatric Urology and Nephrology Studies
- Ultrasound Imaging and Elastography
- Ultrasound and Cavitation Phenomena
- Spaceflight effects on biology
- Radiation Dose and Imaging
- Cold Fusion and Nuclear Reactions
- Dialysis and Renal Disease Management
- Microbial Inactivation Methods
- Hepatocellular Carcinoma Treatment and Prognosis
- Skin Diseases and Diabetes
- Vascular Procedures and Complications
- Biomedical and Chemical Research
- Photoacoustic and Ultrasonic Imaging
- Elasticity and Material Modeling
- Tissue Engineering and Regenerative Medicine
- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- Collagen: Extraction and Characterization
- MicroRNA in disease regulation
- Effects of Radiation Exposure
- Spectroscopy Techniques in Biomedical and Chemical Research
- Renal cell carcinoma treatment
- Liver Disease Diagnosis and Treatment
University of Washington Applied Physics Laboratory
2009-2025
Seattle University
2011-2024
University of Washington
2002-2016
Institute for Systems Biology
2016
Cancer Research Center
2016
Fred Hutch Cancer Center
2016
Significance High intensity focused ultrasound (HIFU) therapy is a promising, clinically adopted method of noninvasive tissue ablation used to treat both benign and malignant conditions. This work presents, our knowledge, the first in vivo validation previously developed HIFU-based that allows for fractionation targeted into subcellular debris—boiling histotripsy—in large animal model. While fractionating soft tissue, boiling histotripsy shown spare adjacent connective structures such as...
Abstract Boiling histotripsy (BH) is a High Intensity Focused Ultrasound (HIFU) method for precise mechanical disintegration of target tissue using millisecond-long pulses containing shocks. BH treatments with real-time ultrasound (US) guidance allowed by BH-generated bubbles were previously demonstrated ex vivo and in exposed porcine liver small animals. Here, the feasibility US-guided transabdominal partially transcostal ablation kidney an acute swine model was evaluated 6 parameters were:...
Background: Diabetic foot ulceration has a complex and multi-factorial etiology can involve changes in the pathophysi-ology of plantar soft tissue. In current study, histomor-phological analyses diabetic non-diabetic tissue were performed. It was hypothesized that would have thicker skin (epidermis dermis), less interdig-itation between dermis epidermis, elastic septa decreased adipose cell size. Materials Methods: Two locations (the heel first metatarsal) examined, both which been reported...
Purpose To compare the abilities of three pulsed focused ultrasound regimes (that cause tissue liquefaction, permeabilization, or mild heating) to release tumor-derived microRNA into circulation in vivo and evaluate dynamics. Materials Methods All rat experiments were approved by University Washington Institutional Animal Care Use Committee. Reverse-transcription quantitative polymerase chain reaction array profiling was used identify candidate biomarkers a solid tumor cell line. Rats...
No AccessJournal of UrologyInvestigative Urology1 Feb 2012Focused Ultrasound to Expel Calculi From the Kidney Anup Shah, Jonathan D. Harper, Bryan W. Cunitz, Yak-Nam Wang, Marla Paun, Julianna C. Simon, Wei Lu, Peter J. Kaczkowski, and Michael R. Bailey ShahAnup Shah More articles by this author , HarperJonathan Harper CunitzBryan Cunitz WangYak-Nam Wang PaunMarla Paun SimonJulianna Simon LuWei Lu KaczkowskiPeter Kaczkowski BaileyMichael View All Author...
No AccessJournal of UrologyInvestigative Urology1 Sep 2013Focused Ultrasound to Expel Calculi from the Kidney: Safety and Efficacy a Clinical Prototype Device Jonathan D. Harper, Mathew Sorensen, Bryan W. Cunitz, Yak-Nam Wang, Julianna C. Simon, Frank Starr, Marla Paun, Barbrina Dunmire, H. Denny Liggitt, Andrew P. Evan, James A. McAteer, Ryan S. Hsi, Michael R. Bailey HarperJonathan Harper Department Urology, School Medicine, University Washington, Seattle, Washington More articles by this...
No AccessJournal of UrologyTranslational Research Article17 Mar 2025Facilitated clearance small, asymptomatic, renal stones with burst wave lithotripsy and ultrasonic propulsion Jonathan D. Harper, Barbrina Dunmire, Jeff Thiel, Barbara H. Burke, Yak-Nam Wang, Stephanie Totten, John C. Kucewicz, Adam Maxwell, M. Kennedy Hall, Arturo E. Holmes, Christina Popchoi, James Lingeman, Alana Desai, Robert Sweet, Manjiri Dighe, Branda J. Levchak, Claire Yang, Michael R. Bailey, Mathew Sorensen...
Boiling histotripsy (BH) is a pulsed high-intensity focused ultrasound (HIFU)-based method of extracorporeal non-thermal tissue disintegration under real-time (US) guidance. Respiratory motion in abdominal targets can affect BH precision and completeness. This study compares two mitigation strategies based on pulse/echo US tracking: robotic arm-based unidirectional compensation by HIFU transducer manipulation pulse gating during expiratory pause. ablations were generated liver kidney...
Focused ultrasonic propulsion is a new noninvasive technique designed to move kidney stones and stone fragments out of the urinary collecting system. However, our knowledge extent tissue injury associated with this not known. We quantitated amount produced by focused under simulated clinical treatment conditions higher power or continuous duty cycles. compared those results extracorporeal shock wave lithotripsy injury.A human calcium oxalate monohydrate and/or nickel beads were implanted...
Molecular imaging, the visualization of molecular and cellular markers, is a promising method for detection dysplasia early cancer in esophagus can potentially be used to identify regions interest biopsy or tumor margins resection. EGFR previously reported cell surface receptor with stepwise increases expression during progression from Barrett's metaplasia adenocarcinoma. In this work, 200 nm fluorescent nanoparticle contrast agent was synthesized targeted imaging through series...
The global prevalence and incidence of renal calculi is reported to be increasing. Of the patients that undergo surgical intervention, nearly half experience symptomatic complications associated with stone fragments are not passed require follow-up intervention. In a clinical simulation using prototype, ultrasonic propulsion was proven effective at repositioning kidney stones in pigs. use ultrasound reposition smaller or location facilitates spontaneous clearance could therefore improve...
Purpose: In the first-in-human trial of ultrasonic propulsion, subjects passed collections residual stone fragments repositioned with a C5-2 probe. Here, effectiveness and safety in moving multiple are compared between custom (SC-50) probe that produces longer focal beam burst duration. Materials Methods: Effectiveness was quantified by number stones expelled from calyx phantom consisting 30-mm deep, water-filled well block tissue mimicking material. Each positioned below to move against...
Pulsed high-intensity focused ultrasound (pHIFU) has the capability to induce de novo cavitation bubbles, offering potential applications for enhancing drug delivery and modulating tissue microenvironments. However, imaging monitoring these bubbles during treatment presents a challenge due their transient nature immediately following pHIFU pulses. A planewave bubble Doppler technique demonstrated its potential, yet this used conventional clutter filter that was originally designed blood flow...
A test apparatus was developed to investigate the effects of mechanical stress application on collagen remodeling in skin. The system maintained a 4.5-cm x 5.5-cm skin explant at an air interface with controlled temperature, relative humidity (RH), and carbon dioxide concentration [CO2] while allowing compressive shear forces be applied surface. For environmental control, custom-designed flow under Proportional-Integral-Derivative (PID) control used. Evaluation tests demonstrated that above...