A5046866683- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Heat shock proteins research
- Lipid metabolism and biosynthesis
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- Plant biochemistry and biosynthesis
- Ubiquitin and proteasome pathways
- Animal Virus Infections Studies
University of Amsterdam
2020-2021
Netherlands Institute for Neuroscience
2021
Geminiviruses are plant-infecting DNA viruses that reshape the intracellular environment of their host in order to create favorable conditions for viral replication and propagation. Viral manipulation is largely mediated via interactions between proteins. Identification this protein network helps us understand how these manipulate therefore provides potentially with novel leads resistance against class pathogens, as genetic variation corresponding plant genes could subvert manipulation....
To identify host factors for tomato spotted wilt orthotospovirus (TSWV), a virus-induced gene silencing (VIGS) screen using tobacco rattle virus (TRV) was performed on Nicotiana benthamiana TSWV susceptibility. rule out any negative effect the plants' performance due to double viral infection, method optimized allow screening of hundreds clones in standardized fashion. normalize results obtained and between experiments, set controls developed evaluate consist manner both VIGS efficacy level...
The tripartite genome of the negative-stranded RNA virus Tomato spotted wilt orthotospovirus (TSWV) is assembled, together with two viral proteins, nucleocapsid protein and RNA-dependent polymerase, into infectious ribonucleoprotein complexes (RNPs). These proteins are, together, essential for replication transcription, yet our knowledge on host factors supporting these processes remains limited. To fill this gap, composition RNPs collected from TSWV-infected Nicotiana benthamiana plants,...
SUMO is a protein modification whose conjugate levels peak during acute heat stress. We find that also critical for plant longevity when Arabidopsis experiences prolonged non-damaging period of only 28 degrees Celsius. Remarkably, this thermo-lethality at was not seen with any other mutant the pathway tested. Autoimmunity due to low SUMO1/2 expression causal thermo-lethality. The role thermo-resilience distinct from its requirement thermomorphogenesis - growth response triggered by same warm...
Abstract Short heat waves (>37°C) are extremely damaging to non-acclimated plants and their capacity recover from stress is key for survival. To acclimate, the HEAT SHOCK TRANSCRIPTION FACTOR A1 (HSFA1) subfamily activates a transcriptional response that resolves incurred damages. In contrast, little known how acclimate sustained non-detrimental warm periods at 27-28°C. Plants respond this condition with thermomorphogenesis response. addition, HSFA1 critical plant survival during these...