A5037482325- Obstructive Sleep Apnea Research
- Intestinal and Peritoneal Adhesions
- Bacterial Identification and Susceptibility Testing
- Sepsis Diagnosis and Treatment
- RNA Research and Splicing
- Intestinal Malrotation and Obstruction Disorders
- Hernia repair and management
- Molecular Biology Techniques and Applications
- Gut microbiota and health
Brown University
2022-2024
Rhode Island Hospital
2024
Alternative splicing (AS) and nonsense-mediated mRNA decay (NMD) are highly conserved cellular mechanisms that modulate gene expression. Here we introduce NMD pipeline computes how events premature termination codons to transcripts via frameshift, then predicts the rate of PTC-dependent NMD. We utilize whole blood, deep RNA-sequencing data from critically ill patients study expression in sepsis. Statistical significance was determined as adjusted p value < 0.05 |log2foldchange| > 2 for...
<title>Abstract</title> Diagnosis of infection in patients with sepsis takes days via culture and appropriate treatment pathogens are delayed awaiting results. A faster diagnosis the pathogen resistance RNA sequencing informed PCR will improve outcomes. We hypothesize that we can use from to identify novel targets for future nucleic acid-based tests. This cohort study 46 admitted ICU samples taken on 0, 1, 3, 7 follow up through hospital stay during 2021–2022. All had sequencing, depth >...